TAF5L (NM_001025247) Human Tagged ORF Clone
CAT#: RC207751
TAF5L (Myc-DDK-tagged)-Human TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa (TAF5L), transcript variant 2
"NM_001025247" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TAF5L |
Synonyms | PAF65B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207751 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACGAGTGCGTACCGAGCAGATTCAGATGGCAGTGTCCTGCTACCTCAAACGCCGGCAGTACGTGG ACTCAGATGGTCCCCTGAAGCAAGGACTGCGGCTGTCACAGACTGCTGAAGAGATGGCGGCCAATCTAAC AGTGCAATCAGAATCTGGTTGTGCCAACATAGTGTCTGCAGCCCCTTGCCAGGCAGAACCCCAGCAATAT GAAGTACAGTTTGGACGACTGCGGAATTTTCTCACTGATTCTGATTCCCAGCATAGCCACGAAGTGATGC CTCTCCTCTATCCTCTCTTTGTCTACCTCCATCTCAACCTGGTCCAAAACAGTCCGAAGAGCACAGTGGA AAGTTTTTACAGCCGCTTCCATGGAATGTTTCTGCAGAATGCTAGCCAGAAGGATGTCATTGAGCAGCTA CAGACCACTCAAACCATCCAGGACATCCTATCTAACTTCAAGCTTCGAGCATTCCTAGATAACAAGTACG TGGTCCGTCTCCAAGAAGACAGCTACAACTACCTTATCCGCTACCTCCAAAGTGACAACAATACTGCCCT GTGCAAAGTCCTCACCTTACATATTCATCTTGACGTGCAGCCTGCCAAGAGAACAGACTATCAGCTGTAT GCCAGTGGCAGCTCCTCCCGCAGTGAGAACAACGGTTTGGAGCCCCCTGACATGCCCAGCCCTATTCTGC AGAACGAGGCTGCCCTAGAGGTCTTACAGGAGAGCATTAAGCGCGTCAAGGATGGGCCTCCCTCCCTCAC TACCATCTGCTTCTATGCCTTCTATAACACAGAGCAGCTGTTGAACACTGCAGAAATCTCCCCCGATAGC AAGCTGCTTGCTGCTGGGTTTGACAACTCCTGTATAAAACTTTGGAGTTTACGATCCAAGAAGTTAAAAT CAGAGCCCCACCAAGTAGACGTGTCCCGCATCCATTTGGCTTGTGATATTCTGGAGGAGGAGGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207751 protein sequence
Red=Cloning site Green=Tags(s) MKRVRTEQIQMAVSCYLKRRQYVDSDGPLKQGLRLSQTAEEMAANLTVQSESGCANIVSAAPCQAEPQQY EVQFGRLRNFLTDSDSQHSHEVMPLLYPLFVYLHLNLVQNSPKSTVESFYSRFHGMFLQNASQKDVIEQL QTTQTIQDILSNFKLRAFLDNKYVVRLQEDSYNYLIRYLQSDNNTALCKVLTLHIHLDVQPAKRTDYQLY ASGSSSRSENNGLEPPDMPSPILQNEAALEVLQESIKRVKDGPPSLTTICFYAFYNTEQLLNTAEISPDS KLLAAGFDNSCIKLWSLRSKKLKSEPHQVDVSRIHLACDILEEEV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001025247 |
ORF Size | 975 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001025247.1, NP_001020418.1 |
RefSeq Size | 4139 bp |
RefSeq ORF | 978 bp |
Locus ID | 27097 |
Protein Families | Transcription Factors |
Protein Pathways | Basal transcription factors |
MW | 37 kDa |
Gene Summary | The product of this gene belongs to the WD-repeat TAF5 family of proteins. This gene encodes a protein that is a component of the PCAF histone acetylase complex. The PCAF histone acetylase complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for myogenic transcription and differentiation. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors to facilitate complex assembly and transcription initiation. The encoded protein is structurally similar to one of the histone-like TAFs, TAF5. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC302381 | TAF5L (untagged)-Human TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa (TAF5L), transcript variant 2 |
USD 660.00 |
|
RG207751 | TAF5L (GFP-tagged) - Human TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa (TAF5L), transcript variant 2 |
USD 460.00 |
|
RC207751L3 | Lenti ORF clone of Human TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa (TAF5L), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC207751L4 | Lenti ORF clone of Human TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa (TAF5L), transcript variant 2, mGFP tagged |
USD 620.00 |
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