EXOC8 (NM_175876) Human Tagged ORF Clone

CAT#: RC207859

EXOC8 (Myc-DDK-tagged)-Human exocyst complex component 8 (EXOC8)

Product Images

Western blot validation of overexpression lysate (Cat# LY406195) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207859 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EXOC8 protein (Cat# TP307859). The protein was produced from HEK293T cells transfected with EXOC8 cDNA clone (Cat# RC207859) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EXOC8
• Synonyms: EXO84; Exo84p; SEC84
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207859 representing NM_175876
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATGGCGATGTCGGACAGTGGGGCGAGCCGCCTGCGTCGGCAGCTGGAGTCAGGGGGTTTTGAGG
  CGCGGCTGTACGTGAAGCAGCTCTCGCAGCAGTCGGATGGGGACCGGGACCTCCAGGAGCACCGGCAGCG
  CATCCAGGCGCTGGCGGAGGAGACGGCGCAGAACCTGAAGCGCAACGTCTACCAGAACTACCGGCAGTTC
  ATAGAGACGGCCCGCGAGATCTCCTACCTGGAGAGCGAGATGTACCAGCTCAGCCATTTGCTGACCGAGC
  AGAAAAGCAGCCTGGAGAGCATCCCGCTTACGTTGCTGCCTGCCGCTGCTGCCGCCGGAGCCGCCGCCGC
  CTCTGGAGGGGAGGAGGGAGTCGGTGGGGCGGGGGGCCGAGACCACCTCCGAGGCCAGGCCGGCTTTTTC
  TCCACCCCCGGGGGTGCCTCCCGCGACGGCTCCGGTCCAGGCGAGGAAGGAAAGCAGCGCACTCTCACCA
  CCCTGCTTGAGAAGGTGGAAGGCTGCAGGCATCTGCTGGAGACGCCGGGACAGTACTTGGTGTACAATGG
  GGACCTAGTGGAATACGATGCGGACCACATGGCCCAACTGCAGCGGGTGCACGGCTTTCTCATGAACGAT
  TGCTTGTTGGTGGCTACCTGGCTGCCTCAGCGGCGTGGGATGTATCGCTACAACGCTCTCTATTCCCTAG
  ATGGTTTGGCCGTAGTCAATGTCAAGGACAACCCGCCCATGAAGGACATGTTCAAGCTGCTTATGTTCCC
  CGAGAGCCGTATTTTCCAGGCCGAAAATGCTAAAATCAAACGAGAGTGGCTGGAAGTGCTGGAGGACACC
  AAGAGGGCCCTCAGTGAGAAAAGGCGAAGGGAGCAGGAGGAGGCAGCGGCCCCTCGAGGGCCACCCCAAG
  TGACTTCCAAGGCCACTAACCCATTTGAGGATGACGAAGAAGAAGAACCAGCTGTTCCTGAGGTAGAGGA
  AGAGAAGGTGGACCTCTCCATGGAATGGATCCAGGAGTTACCTGAAGACCTGGATGTCTGCATTGCGCAG
  AGAGACTTTGAAGGGGCGGTTGACCTGCTGGATAAATTGAACCATTACCTGGAAGATAAACCTAGCCCAC
  CTCCTGTAAAAGAACTAAGGGCCAAAGTGGAGGAGCGAGTTCGACAGCTCACTGAGGTGCTAGTTTTCGA
  ACTCTCCCCAGATCGTTCCCTGAGAGGTGGTCCGAAGGCTACTCGCAGAGCAGTTTCGCAACTGATCCGG
  CTGGGCCAGTGCACGAAGGCCTGTGAGCTATTTTTGAGAAACAGGGCAGCCGCTGTTCATACTGCAATTC
  GTCAGCTTCGCATCGAAGGTGCCACTTTACTCTATATTCATAAGCTGTGCCATGTCTTCTTTACCAGCCT
  TCTCGAGACTGCAAGAGAATTTGAGATCGATTTTGCAGGCACTGACAGCGGCTGCTACTCTGCCTTTGTG
  GTCTGGGCAAGATCAGCCATGGGCATGTTCGTGGATGCTTTTAGCAAGCAGGTGTTTGATAGTAAGGAGA
  GCCTCTCTACAGCAGCTGAGTGTGTAAAAGTGGCTAAGGAGCATTGCCAGCAACTGGGTGATATCGGACT
  GGATCTCACCTTCATCATCCATGCCCTTCTGGTGAAAGACATCCAAGGGGCCTTGCACAGTTACAAAGAA
  ATCATCATTGAAGCCACTAAACATCGCAACTCTGAAGAGATGTGGAGGAGGATGAACTTGATGACGCCAG
  AAGCCCTGGGTAAGCTCAAAGAAGAGATGAAAAGTTGTGGGGTAAGTAACTTTGAGCAGTACACAGGGGA
  TGACTGCTGGGTGAACCTAAGTTACACAGTGGTTGCTTTCACCAAACAGACCATGGGCTTCTTGGAAGAG
  GCCCTGAAGCTGTATTTCCCAGAGCTGCACATGGTACTTTTGGAGAGCCTGGTGGAAATCATTTTGGTTG
  CTGTTCAGCATGTGGATTATAGTCTTCGATGTGAGCAGGATCCAGAGAAGAAAGCTTTTATCAGACAGAA
  TGCATCCTTTTTATATGAAACAGTCCTCCCTGTGGTGGAGAAAAGGTTTGAAGAAGGTGTGGGGAAACCT
  GCCAAGCAACTCCAAGATCTGAGGAATGCATCTAGACTTATTCGTGTGAATCCTGAAAGTACAACATCAG
  TGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207859 representing NM_175876
  Red=Cloning site Green=Tags(s)
  
  MAMAMSDSGASRLRRQLESGGFEARLYVKQLSQQSDGDRDLQEHRQRIQALAEETAQNLKRNVYQNYRQF
  IETAREISYLESEMYQLSHLLTEQKSSLESIPLTLLPAAAAAGAAAASGGEEGVGGAGGRDHLRGQAGFF
  STPGGASRDGSGPGEEGKQRTLTTLLEKVEGCRHLLETPGQYLVYNGDLVEYDADHMAQLQRVHGFLMND
  CLLVATWLPQRRGMYRYNALYSLDGLAVVNVKDNPPMKDMFKLLMFPESRIFQAENAKIKREWLEVLEDT
  KRALSEKRRREQEEAAAPRGPPQVTSKATNPFEDDEEEEPAVPEVEEEKVDLSMEWIQELPEDLDVCIAQ
  RDFEGAVDLLDKLNHYLEDKPSPPPVKELRAKVEERVRQLTEVLVFELSPDRSLRGGPKATRRAVSQLIR
  LGQCTKACELFLRNRAAAVHTAIRQLRIEGATLLYIHKLCHVFFTSLLETAREFEIDFAGTDSGCYSAFV
  VWARSAMGMFVDAFSKQVFDSKESLSTAAECVKVAKEHCQQLGDIGLDLTFIIHALLVKDIQGALHSYKE
  IIIEATKHRNSEEMWRRMNLMTPEALGKLKEEMKSCGVSNFEQYTGDDCWVNLSYTVVAFTKQTMGFLEE
  ALKLYFPELHMVLLESLVEIILVAVQHVDYSLRCEQDPEKKAFIRQNASFLYETVLPVVEKRFEEGVGKP
  AKQLQDLRNASRLIRVNPESTTSVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175876
• ORF Size: 2175 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175876.2, NM_175876.3, NM_175876.4, NP_787072.2
• RefSeq Size: 5099
• RefSeq ORF: 2178
• Locus ID: 149371
• MW: 81.6 kDa
• Gene Summary: This gene encodes a component of the exocyst complex, an evolutionarily conserved multi-protein complex that plays a critical role in vesicular trafficking and the secretory pathway by targeting post-Golgi vesicles to the plasma membrane. This protein is a target of activated Ral subfamily of GTPases and thereby regulates exocytosis by tethering vesicles to the plasma membrane. Mutations in this gene may be related to Joubert syndrome. [provided by RefSeq, Sep 2016]