DTX3L (NM_138287) Human Tagged ORF Clone

CAT#: RC207944

DTX3L (Myc-DDK-tagged)-Human deltex 3-like (Drosophila) (DTX3L)

Product Images

Western blot validation of overexpression lysate (Cat# LY403351) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207944 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DTX3L protein (Cat# TP307944). The protein was produced from HEK293T cells transfected with DTX3L cDNA clone (Cat# RC207944) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DTX3L
• Synonyms: BBAP; RNF143
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207944 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCCACCTGCGCCCGCCGTCCCCGCTCCTCGTGCGGGTGTACAAGTCCGGCCCCCGAGTACGAA
  GGAAGCTGGAGAGCTACTTCCAGAGCTCTAAGTCCTCGGGCGGCGGGGAGTGCACGGTCAGCACCCAGGA
  ACACGAAGCCCCGGGCACCTTCCGGGTGGAGTTCAGTGAAAGGGCAGCTAAGGAGAGAGTGTTGAAAAAA
  GGAGAGCACCAAATACTTGTTGACGAAAAACCTGTGCCCATTTTCCTGGTACCCACTGAAAATTCAATAA
  AGAAGAACACGAGACCTCAAATTTCTTCACTGACACAATCACAAGCAGAAACACCGTCTGGTGATATGCA
  TCAACATGAAGGACATATTCCTAATGCTGTGGATTCCTGTCTCCAAAAGATCTTTCTTACTGTAACAGCT
  GACCTGAACTGTAACCTGTTCTCCAAAGAGCAGAGGGCATACATAACCACACTGTGCCCTAGTATCAGAA
  AAATGGAAGGTCACGATGGAATTGAGAAGGTGTGTGGTGACTTCCAAGACATTGAAAGAATACATCAATT
  TTTGAGTGAGCAGTTCCTGGAAAGTGAGCAGAAACAACAATTTTCCCCTTCAATGACAGAGAGGAAGCCA
  CTCAGTCAGCAGGAGAGGGACAGCTGCATTTCTCCTTCTGAACCAGAAACCAAGGCAGAACAAAAAAGCA
  ACTATTTTGAAGTTCCCTTGCCTTACTTTGAATACTTTAAATATATCTGTCCTGATAAAATCAACTCAAT
  AGAGAAAAGATTTGGTGTAAACATTGAAATCCAGGAGAGTTCTCCAAATATGGTCTGTTTAGATTTCACC
  TCAAGTCGATCAGGTGACCTGGAAGCAGCTCGTGAGTCTTTTGCTAGTGAATTTCAGAAGAACACAGAAC
  CTCTGAAGCAAGAATGTGTCTCTTTAGCAGACAGTAAGCAGGCAAATAAATTCAAACAGGAATTGAATCA
  CCAGTTTACAAAGCTCCTTATAAAGGAGAAAGGAGGCGAATTAACTCTCCTTGGGACCCAAGATGACATT
  TCAGCTGCCAAACAAAAAATCTCTGAAGCTTTTGTCAAGATACCTGTGAAACTATTTGCTGCCAATTACA
  TGATGAATGTAATTGAGGTTGATAGTGCCCACTATAAACTTTTAGAAACTGAATTACTACAGGAGATATC
  AGAGATCGAAAAAAGGTATGACATTTGCAGCAAGGTTTCTGAGAAAGGTCAGAAAACCTGCATTCTGTTT
  GAATCCAAGGACAGGCAGGTAGATCTATCTGTGCATGCTTATGCAAGTTTCATCGATGCCTTTCAACATG
  CCTCATGTCAGTTGATGAGAGAAGTTCTTTTACTGAAGTCTTTGGGCAAGGAGAGAAAGCACTTACATCA
  GACCAAGTTTGCTGATGACTTTAGAAAAAGACATCCAAATGTACACTTTGTGCTAAATCAAGAGTCAATG
  ACTTTGACTGGTTTGCCAAATCACCTTGCAAAGGCGAAGCAGTATGTTCTAAAAGGAGGAGGAATGTCTT
  CATTGGCTGGAAAGAAATTGAAAGAGGGTCATGAAACACCGATGGACATTGATAGCGATGATTCCAAAGC
  AGCTTCTCCGCCACTCAAGGGCTCTGTGAGTTCTGAGGCCTCAGAACTGGACAAGAAGGAAAAGGGCATC
  TGTGTCATCTGTATGGACACCATTAGTAACAAAAAAGTGCTACCAAAGTGCAAGCATGAATTCTGCGCCC
  CTTGTATCAACAAAGCCATGTCATATAAGCCAATCTGTCCCACATGCCAGACTTCCTATGGTATTCAGAA
  AGGAAATCAGCCAGAGGGAAGCATGGTTTTCACTGTTTCAAGAGACTCACTTCCAGGTTATGAGTCCTTT
  GGCACCATTGTGATTACTTATTCTATGAAAGCAGGCATACAAACAGAAGAACACCCAAACCCAGGAAAGA
  GATACCCTGGAATACAGCGAACTGCATACTTGCCTGATAATAAGGAAGGAAGGAAGGTTTTGAAACTGCT
  TTATAGGGCCTTTGACCAAAAGCTGATTTTTACAGTGGGGTACTCTCGCGTATTAGGAGTCTCAGATGTC
  ATCACTTGGAATGATATTCACCACAAAACATCCCGGTTTGGAGGACCAGAAATGTATGGCTATCCTGATC
  CTTCTTACCTGAAACGTGTCAAAGAGGAGCTGAAAGCCAAAGGAATTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207944 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASHLRPPSPLLVRVYKSGPRVRRKLESYFQSSKSSGGGECTVSTQEHEAPGTFRVEFSERAAKERVLKK
  GEHQILVDEKPVPIFLVPTENSIKKNTRPQISSLTQSQAETPSGDMHQHEGHIPNAVDSCLQKIFLTVTA
  DLNCNLFSKEQRAYITTLCPSIRKMEGHDGIEKVCGDFQDIERIHQFLSEQFLESEQKQQFSPSMTERKP
  LSQQERDSCISPSEPETKAEQKSNYFEVPLPYFEYFKYICPDKINSIEKRFGVNIEIQESSPNMVCLDFT
  SSRSGDLEAARESFASEFQKNTEPLKQECVSLADSKQANKFKQELNHQFTKLLIKEKGGELTLLGTQDDI
  SAAKQKISEAFVKIPVKLFAANYMMNVIEVDSAHYKLLETELLQEISEIEKRYDICSKVSEKGQKTCILF
  ESKDRQVDLSVHAYASFIDAFQHASCQLMREVLLLKSLGKERKHLHQTKFADDFRKRHPNVHFVLNQESM
  TLTGLPNHLAKAKQYVLKGGGMSSLAGKKLKEGHETPMDIDSDDSKAASPPLKGSVSSEASELDKKEKGI
  CVICMDTISNKKVLPKCKHEFCAPCINKAMSYKPICPTCQTSYGIQKGNQPEGSMVFTVSRDSLPGYESF
  GTIVITYSMKAGIQTEEHPNPGKRYPGIQRTAYLPDNKEGRKVLKLLYRAFDQKLIFTVGYSRVLGVSDV
  ITWNDIHHKTSRFGGPEMYGYPDPSYLKRVKEELKAKGIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138287
• ORF Size: 2220 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138287.1, NM_138287.2, NM_138287.3, NP_612144.1
• RefSeq Size: 5768 bp
• RefSeq ORF: 2223 bp
• Locus ID: 151636
• Domains: RING
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• MW: 83.6 kDa
• Gene Summary: DTX3L functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]). [supplied by OMIM, Nov 2009]