DTX3L (NM_138287) Human Tagged ORF Clone

CAT#: RG207944

DTX3L (GFP-tagged) - Human deltex 3-like (Drosophila) (DTX3L)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DTX3L
• Synonyms: BBAP; RNF143
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207944 representing NM_138287
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCCACCTGCGCCCGCCGTCCCCGCTCCTCGTGCGGGTGTACAAGTCCGGCCCCCGAGTACGAA
  GGAAGCTGGAGAGCTACTTCCAGAGCTCTAAGTCCTCGGGCGGCGGGGAGTGCACGGTCAGCACCCAGGA
  ACACGAAGCCCCGGGCACCTTCCGGGTGGAGTTCAGTGAAAGGGCAGCTAAGGAGAGAGTGTTGAAAAAA
  GGAGAGCACCAAATACTTGTTGACGAAAAACCTGTGCCCATTTTCCTGGTACCCACTGAAAATTCAATAA
  AGAAGAACACGAGACCTCAAATTTCTTCACTGACACAATCACAAGCAGAAACACCGTCTGGTGATATGCA
  TCAACATGAAGGACATATTCCTAATGCTGTGGATTCCTGTCTCCAAAAGATCTTTCTTACTGTAACAGCT
  GACCTGAACTGTAACCTGTTCTCCAAAGAGCAGAGGGCATACATAACCACACTGTGCCCTAGTATCAGAA
  AAATGGAAGGTCACGATGGAATTGAGAAGGTGTGTGGTGACTTCCAAGACATTGAAAGAATACATCAATT
  TTTGAGTGAGCAGTTCCTGGAAAGTGAGCAGAAACAACAATTTTCCCCTTCAATGACAGAGAGGAAGCCA
  CTCAGTCAGCAGGAGAGGGACAGCTGCATTTCTCCTTCTGAACCAGAAACCAAGGCAGAACAAAAAAGCA
  ACTATTTTGAAGTTCCCTTGCCTTACTTTGAATACTTTAAATATATCTGTCCTGATAAAATCAACTCAAT
  AGAGAAAAGATTTGGTGTAAACATTGAAATCCAGGAGAGTTCTCCAAATATGGTCTGTTTAGATTTCACC
  TCAAGTCGATCAGGTGACCTGGAAGCAGCTCGTGAGTCTTTTGCTAGTGAATTTCAGAAGAACACAGAAC
  CTCTGAAGCAAGAATGTGTCTCTTTAGCAGACAGTAAGCAGGCAAATAAATTCAAACAGGAATTGAATCA
  CCAGTTTACAAAGCTCCTTATAAAGGAGAAAGGAGGCGAATTAACTCTCCTTGGGACCCAAGATGACATT
  TCAGCTGCCAAACAAAAAATCTCTGAAGCTTTTGTCAAGATACCTGTGAAACTATTTGCTGCCAATTACA
  TGATGAATGTAATTGAGGTTGATAGTGCCCACTATAAACTTTTAGAAACTGAATTACTACAGGAGATATC
  AGAGATCGAAAAAAGGTATGACATTTGCAGCAAGGTTTCTGAGAAAGGTCAGAAAACCTGCATTCTGTTT
  GAATCCAAGGACAGGCAGGTAGATCTATCTGTGCATGCTTATGCAAGTTTCATCGATGCCTTTCAACATG
  CCTCATGTCAGTTGATGAGAGAAGTTCTTTTACTGAAGTCTTTGGGCAAGGAGAGAAAGCACTTACATCA
  GACCAAGTTTGCTGATGACTTTAGAAAAAGACATCCAAATGTACACTTTGTGCTAAATCAAGAGTCAATG
  ACTTTGACTGGTTTGCCAAATCACCTTGCAAAGGCGAAGCAGTATGTTCTAAAAGGAGGAGGAATGTCTT
  CATTGGCTGGAAAGAAATTGAAAGAGGGTCATGAAACACCGATGGACATTGATAGCGATGATTCCAAAGC
  AGCTTCTCCGCCACTCAAGGGCTCTGTGAGTTCTGAGGCCTCAGAACTGGACAAGAAGGAAAAGGGCATC
  TGTGTCATCTGTATGGACACCATTAGTAACAAAAAAGTGCTACCAAAGTGCAAGCATGAATTCTGCGCCC
  CTTGTATCAACAAAGCCATGTCATATAAGCCAATCTGTCCCACATGCCAGACTTCCTATGGTATTCAGAA
  AGGAAATCAGCCAGAGGGAAGCATGGTTTTCACTGTTTCAAGAGACTCACTTCCAGGTTATGAGTCCTTT
  GGCACCATTGTGATTACTTATTCTATGAAAGCAGGCATACAAACAGAAGAACACCCAAACCCAGGAAAGA
  GATACCCTGGAATACAGCGAACTGCATACTTGCCTGATAATAAGGAAGGAAGGAAGGTTTTGAAACTGCT
  TTATAGGGCCTTTGACCAAAAGCTGATTTTTACAGTGGGGTACTCTCGCGTATTAGGAGTCTCAGATGTC
  ATCACTTGGAATGATATTCACCACAAAACATCCCGGTTTGGAGGACCAGAAATGTATGGCTATCCTGATC
  CTTCTTACCTGAAACGTGTCAAAGAGGAGCTGAAAGCCAAAGGAATTGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207944 representing NM_138287
  Red=Cloning site Green=Tags(s)
  
  MASHLRPPSPLLVRVYKSGPRVRRKLESYFQSSKSSGGGECTVSTQEHEAPGTFRVEFSERAAKERVLKK
  GEHQILVDEKPVPIFLVPTENSIKKNTRPQISSLTQSQAETPSGDMHQHEGHIPNAVDSCLQKIFLTVTA
  DLNCNLFSKEQRAYITTLCPSIRKMEGHDGIEKVCGDFQDIERIHQFLSEQFLESEQKQQFSPSMTERKP
  LSQQERDSCISPSEPETKAEQKSNYFEVPLPYFEYFKYICPDKINSIEKRFGVNIEIQESSPNMVCLDFT
  SSRSGDLEAARESFASEFQKNTEPLKQECVSLADSKQANKFKQELNHQFTKLLIKEKGGELTLLGTQDDI
  SAAKQKISEAFVKIPVKLFAANYMMNVIEVDSAHYKLLETELLQEISEIEKRYDICSKVSEKGQKTCILF
  ESKDRQVDLSVHAYASFIDAFQHASCQLMREVLLLKSLGKERKHLHQTKFADDFRKRHPNVHFVLNQESM
  TLTGLPNHLAKAKQYVLKGGGMSSLAGKKLKEGHETPMDIDSDDSKAASPPLKGSVSSEASELDKKEKGI
  CVICMDTISNKKVLPKCKHEFCAPCINKAMSYKPICPTCQTSYGIQKGNQPEGSMVFTVSRDSLPGYESF
  GTIVITYSMKAGIQTEEHPNPGKRYPGIQRTAYLPDNKEGRKVLKLLYRAFDQKLIFTVGYSRVLGVSDV
  ITWNDIHHKTSRFGGPEMYGYPDPSYLKRVKEELKAKGIE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138287
• ORF Size: 2220 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138287.2, NP_612144.1
• RefSeq Size: 4926
• RefSeq ORF: 2223
• Locus ID: 151636
• Domains: RING
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• Gene Summary: DTX3L functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]). [supplied by OMIM, Nov 2009]