FAM20C (NM_020223) Human Tagged ORF Clone

CAT#: RC207945

FAM20C (Myc-DDK-tagged)-Human family with sequence similarity 20, member C (FAM20C)

Product Images

Western blot validation of overexpression lysate (Cat# LY412584) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207945 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM20C
• Synonyms: DMP-4; DMP4; G-CK; GEF-CK; RNS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207945 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTCCTGGTGGCCTGCGCGCTGCACATCGCCCTGGACCTGCTGCCCAGGCTGGAGCGACGCGGCG
  CGCGGCCCTCGGGGGAGCCCGGCTGTTCGTGCGCGCAGCCCGCCGCCGAGGTGGCCGCGCCCGGCTGGGC
  CCAGGTTCGGGGCCGCCCCGGGGAGCCCCCGGCCGCCTCCTCCGCCGCCGGCGACGCGGGCTGGCCCAAC
  AAGCACACGCTCCGCATCCTGCAGGACTTCAGCTCCGACCCCTCCTCCAACCTCTCGTCCCACTCGCTGG
  AGAAACTGCCGCCCGCGGCCGAGCCGGCCGAGCGCGCCTTGCGGGGGCGGGATCCCGGCGCCCTAAGACC
  CCACGACCCCGCGCACCGGCCGCTGCTGCGAGACCCCGGCCCGCGTCGGTCCGAGTCGCCCCCCGGCCCC
  GGCGGAGACGCCTCCCTCCTGGCCAGGCTGTTCGAGCACCCGCTTTACCGGGTGGCGGTTCCGCCGCTCA
  CGGAGGAGGACGTCCTGTTCAATGTGAACAGCGACACCAGGCTCAGCCCCAAAGCGGCGGAGAACCCGGA
  CTGGCCGCATGCGGGTGCTGAAGGTGCAGAATTCCTCTCCCCCGGGGAGGCGGCCGTGGACTCCTATCCC
  AACTGGCTCAAGTTCCACATTGGTATCAACCGGTACGAGCTGTACTCCAGACACAACCCGGCCATCGAGG
  CCCTGCTGCACGACCTCAGCTCCCAGAGGATCACCAGCGTGGCCATGAAGTCGGGGGGCACGCAGCTGAA
  GCTCATCATGACCTTCCAGAATTACGGGCAAGCGCTGTTCAAACCCATGAAACAAACGAGGGAGCAGGAG
  ACACCCCCTGACTTTTTTTATTTCTCTGACTACGAGAGGCACAATGCGGAGATTGCTGCCTTCCACCTGG
  ACAGGATCCTGGACTTCCGCCGGGTCCCTCCCGTGGCCGGCAGGATGGTCAACATGACCAAGGAGATCCG
  GGACGTCACACGGGACAAGAAGCTCTGGAGGACCTTCTTCATCTCTCCAGCCAACAACATCTGCTTCTAC
  GGCGAGTGTTCCTACTACTGCTCCACGGAGCACGCCCTGTGCGGGAAGCCAGACCAGATCGAGGGCTCGC
  TGGCGGCCTTCCTGCCCGACCTGTCCCTGGCCAAGAGGAAGACCTGGCGGAACCCTTGGCGGCGTTCCTA
  CCACAAGCGCAAGAAGGCCGAGTGGGAGGTGGACCCTGACTACTGCGAGGAGGTGAAGCAGACACCGCCC
  TACGACAGCAGCCACCGCATCCTGGACGTCATGGACATGACGATCTTCGACTTCCTCATGGGAAACATGG
  ACCGTCACCACTACGAGACTTTTGAGAAGTTTGGGAATGAAACGTTCATCATCCACTTAGACAATGGAAG
  AGGGTTTGGGAAGTATTCGCACGACGAGCTCTCCATCCTGGTGCCGCTACAGCAGTGCTGCAGGATCCGG
  AAGTCCACCTACCTGCGTCTGCAGCTCCTGGCCAAGGAGGAGTACAAGCTGAGCCTGCTGATGGCCGAGT
  CTCTGCGGGGGGACCAGGTGGCACCCGTGCTGTACCAGCCGCACCTGGAGGCCCTGGACCGGCGGCTCCG
  CGTCGTGCTAAAGGCCGTCCGGGACTGCGTGGAGAGGAACGGGCTCCACAGCGTGGTGGATGACGACCTG
  GACACTGAGCACAGAGCCGCCTCGGCGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207945 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVFLVACALHIALDLLPRLERRGARPSGEPGCSCAQPAAEVAAPGWAQVRGRPGEPPAASSAAGDAGWPN
  KHTLRILQDFSSDPSSNLSSHSLEKLPPAAEPAERALRGRDPGALRPHDPAHRPLLRDPGPRRSESPPGP
  GGDASLLARLFEHPLYRVAVPPLTEEDVLFNVNSDTRLSPKAAENPDWPHAGAEGAEFLSPGEAAVDSYP
  NWLKFHIGINRYELYSRHNPAIEALLHDLSSQRITSVAMKSGGTQLKLIMTFQNYGQALFKPMKQTREQE
  TPPDFFYFSDYERHNAEIAAFHLDRILDFRRVPPVAGRMVNMTKEIRDVTRDKKLWRTFFISPANNICFY
  GECSYYCSTEHALCGKPDQIEGSLAAFLPDLSLAKRKTWRNPWRRSYHKRKKAEWEVDPDYCEEVKQTPP
  YDSSHRILDVMDMTIFDFLMGNMDRHHYETFEKFGNETFIIHLDNGRGFGKYSHDELSILVPLQQCCRIR
  KSTYLRLQLLAKEEYKLSLLMAESLRGDQVAPVLYQPHLEALDRRLRVVLKAVRDCVERNGLHSVVDDDL
  DTEHRAASAR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020223
• ORF Size: 1710 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020223.1, NM_020223.2, NP_064608.1, NP_064608.2
• RefSeq Size: 2787
• RefSeq ORF: 1755
• Locus ID: 56975
• Protein Families: Secreted Protein, Transmembrane
• MW: 64.4 kDa
• Gene Summary: This gene encodes a member of the family of secreted protein kinases. The encoded protein binds calcium and phosphorylates proteins involved in bone mineralization. Mutations in this gene are associated with the autosomal recessive disorder Raine syndrome. [provided by RefSeq, Apr 2014]