EMX1 (NM_004097) Human Tagged ORF Clone

CAT#: RC208006

EMX1 (Myc-DDK-tagged)-Human empty spiracles homeobox 1 (EMX1)

Product Images

Western blot validation of overexpression lysate (Cat# LY418217) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208006 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EMX1
• Synonyms: empty spiracles homeobox 1; empty spiracles homolog 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208006 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCTGGCTGGGTGCACACCCCGCAAGGCGGCGGCGCCAGGACGCGGAGCGCTCCCCAGAGCACGGC
  TGCCTCGCACAGCTCCCGCGGCTGCGACCATGTTCCAGCCCGCGGCCAAGCGCGGCTTTACCATAGAGTC
  CTTGGTGGCCAAGGACGGCGGCACCGGCGGGGGCACTGGCGGCGGGGGCGCGGGCTCCCATCTCCTGGCG
  GCGGCCGCCTCCGAGGAACCGCTCCGGCCCACGGCGCTCAACTACCCTCACCCCAGCGCGGCCGAGGCGG
  CCTTCGTGAGTGGCTTCCCTGCCGCGGCCGCCGCGGGCGCGGGCCGCTCGCTCTACGGTGGGCCCGAGCT
  CGTGTTCCCCGAGGCCATGAACCACCCCGCGCTGACCGTGCATCCGGCGCACCAGCTGGGCGCCTCCCCG
  CTGCAGCCCCCGCACTCCTTCTTCGGCGCCCAGCACCGGGACCCTCTCCATTTCTACCCCTGGGTCCTGC
  GGAACCGCTTCTTCGGCCACCGCTTCCAGGCCAGCGACGTGCCCCAGGACGGGCTGCTTCTGCACGGCCC
  CTTCGCACGCAAGCCCAAGCGGATCCGCACGGCCTTCTCGCCCTCGCAGCTGCTGCGGCTGGAGCGCGCC
  TTCGAGAAGAACCACTACGTGGTGGGCGCCGAGCGGAAGCAGCTGGCCGGCAGTCTCAGCCTCTCCGAGA
  CGCAGGTGAAGGTGTGGTTCCAGAACCGGAGGACAAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCC
  TGAGTCCGAGCAGAAGAAGAAGGGCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAGGCCAAT
  GGGGAGGACATCGATGTCACCTCCAATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208006 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCLAGCTPRKAAAPGRGALPRARLPRTAPAAATMFQPAAKRGFTIESLVAKDGGTGGGTGGGGAGSHLLA
  AAASEEPLRPTALNYPHPSAAEAAFVSGFPAAAAAGAGRSLYGGPELVFPEAMNHPALTVHPAHQLGASP
  LQPPHSFFGAQHRDPLHFYPWVLRNRFFGHRFQASDVPQDGLLLHGPFARKPKRIRTAFSPSQLLRLERA
  FEKNHYVVGAERKQLAGSLSLSETQVKVWFQNRRTKYKRQKLEEEGPESEQKKKGSHHINRWRIATKQAN
  GEDIDVTSND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004097
• ORF Size: 870 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004097.2, NP_004088.2
• RefSeq Size: 2188 bp
• RefSeq ORF: 873 bp
• Locus ID: 2016
• Cytogenetics: 2p13.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• MW: 31.3 kDa
• Gene Summary: ''