ZNF70 (NM_021916) Human Tagged ORF Clone

CAT#: RC208080

ZNF70 (Myc-DDK-tagged)-Human zinc finger protein 70 (ZNF70). Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF70
• Synonyms: Cos17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208080 ORF sequence, codon optimized.
  Due to the complexity of NM_021916, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTACCACCCGCGACCAAGTTCGGCGAGACATTTGCTTTCGAGAATCGACTGGAGAGCCAGCAGG
  GCCTCTTTCCTGGTGAAGACTTGGGGGATCCCTTCTTGCAAGAGAGAGGCCTGGAACAGATGGCCGTCAT
  TTATAAGGAGATACCCCTGGGGGAGCAAGATGAAGAGAATGATGACTACGAGGGAAATTTTTCCCTCTGC
  TCCAGCCCCGTCCAGCATCAGAGTATCCCACCTGGCACCCGCCCTCAGGACGATGAGCTGTTTGGCCAAA
  CATTTCTGCAAAAGTCCGACTTGTCTATGTGCCAGATTATTCATTCAGAGGAGCCCTCCCCCTGTGATTG
  CGCCGAAACCGACAGAGGCGATTCTGGTCCAAACGCTCCTCATAGAACCCCACAGCCGGCTAAACCATAT
  GCTTGTAGGGAGTGTGGAAAAGCATTTTCCCAGAGTAGCCACCTCTTGCGACACCTCGTGATACACACCG
  GAGAAAAGCCGTATGAGTGCTGCGAGTGCGGCAAGGCTTTCTCCCAAAGTTCTCACCTGCTGAGACACCA
  GATTATCCACACAGGCGAAAAACCTTACGAATGTCGGGAGTGTGGGAAGGCCTTCCGGCAATCCTCTGCA
  TTGACACAACACCAGAAAATCCACACCGGCAAGCGACCTTACGAATGTAGAGAGTGTGGAAAAGATTTCT
  CTCGGAGCAGTTCTCTGCGCAAGCATGAAAGAATACACACCGGAGAGCGACCCTACCAGTGCAAAGAGTG
  TGGAAAAAGTTTCAATCAGAGTTCCGGCCTGAGTCAGCACCGGAAGATCCACACGCTGAAAAAGCCTCAT
  GAATGCGACTTGTGCGGAAAAGCCTTTTGCCACCGCAGCCACCTCATACGGCATCAGCGCATCCACACAG
  GAAAGAAGCCCTACAAGTGCGACGAATGTGGCAAGGCATTCTCCCAGAGCAGTAACCTGATTGAGCACAG
  GAAAACTCACACCGGAGAAAAACCTTATAAATGCCAGAAATGTGGCAAGGCTTTCTCCCAAAGCAGCTCC
  CTCATCGAACACCAGCGCATTCATACAGGTGAGAAACCTTACGAATGCTGCCAGTGCGGCAAGGCCTTCT
  GTCACTCCTCAGCTCTGATACAGCACCAGCGCATCCACACGGGTAAGAAGCCGTACACCTGCGAGTGCGG
  CAAAGCTTTCAGGCACAGGAGTGCACTCATCGAACACTACAAAACACACACCAGAGAAAAACCCTATGTA
  TGTAACTTGTGTGGCAAGTCCTTTAGGGGCAGTTCACATTTGATTCGCCATCAGAAGATTCACAGTGGTG
  AGAAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208080 representing NM_021916
  Red=Cloning site Green=Tags(s)
  
  MEVPPATKFGETFAFENRLESQQGLFPGEDLGDPFLQERGLEQMAVIYKEIPLGEQDEENDDYEGNFSLC
  SSPVQHQSIPPGTRPQDDELFGQTFLQKSDLSMCQIIHSEEPSPCDCAETDRGDSGPNAPHRTPQPAKPY
  ACRECGKAFSQSSHLLRHLVIHTGEKPYECCECGKAFSQSSHLLRHQIIHTGEKPYECRECGKAFRQSSA
  LTQHQKIHTGKRPYECRECGKDFSRSSSLRKHERIHTGERPYQCKECGKSFNQSSGLSQHRKIHTLKKPH
  ECDLCGKAFCHRSHLIRHQRIHTGKKPYKCDECGKAFSQSSNLIEHRKTHTGEKPYKCQKCGKAFSQSSS
  LIEHQRIHTGEKPYECCQCGKAFCHSSALIQHQRIHTGKKPYTCECGKAFRHRSALIEHYKTHTREKPYV
  CNLCGKSFRGSSHLIRHQKIHSGEKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021916
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021916.1, NM_021916.2, NM_021916.3, NP_068735.1
• RefSeq Size: 6940 bp
• RefSeq ORF: 1341 bp
• Locus ID: 7621
• Cytogenetics: 22q11.23
• Protein Families: Transcription Factors
• MW: 50.8 kDa
• Gene Summary: ''