BAG5 (NM_001015049) Human Tagged ORF Clone

CAT#: RC208123

BAG5 (Myc-DDK-tagged)-Human BCL2-associated athanogene 5 (BAG5), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BAG5
• Synonyms: BAG-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208123 representing NM_001015049
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTTTCCATTGGTTACCCACCTTGAGCGAGCCGTTTGATAGAAACCAAGAGCTTGAAACGTGCATTA
  GGCCATTGTGGACTCCCAGTGGGAGTGCTTGTGAAACTGAACACAACAAAAGTATGGATATGGGAAACCA
  ACATCCTTCTATTAGTAGGCTTCAGGAAATCCAAAAGGAAGTAAAAAGTGTAGAACAGCAAGTTATCGGC
  TTCAGTGGTCTGTCAGATGACAAGAATTACAAGAAACTGGAGAGGATTCTAACAAAACAGCTTTTTGAAA
  TAGACTCTGTAGATACTGAAGGAAAAGGAGATATTCAGCAAGCTAGGAAGCGGGCAGCACAGGAGACAGA
  ACGTCTTCTCAAAGAGTTGGAGCAGAATGCAAACCACCCACACCGGATTGAAATACAGAACATTTTTGAG
  GAAGCCCAGTCCCTCGTGAGAGAGAAAATTGTGCCATTTTATAATGGAGGCAACTGCGTAACTGATGAGT
  TTGAAGAAGGCATCCAAGATATCATTCTGAGGCTGACACATGTTAAAACTGGAGGAAAAATCTCCTTGCG
  GAAAGCAAGGTATCACACTTTAACCAAAATCTGTGCGGTGCAAGAGATAATCGAAGACTGCATGAAAAAG
  CAGCCTTCCCTGCCGCTTTCCGAGGATGCACATCCTTCCGTTGCCAAAATCAACTTCGTGATGTGTGAGG
  TGAACAAGGCCCGAGGGGTCCTGATTGCACTTCTGATGGGTGTGAACAACAATGAGACCTGCAGGCACTT
  ATCCTGTGTGCTCTCGGGGCTGATCGCTGACCTGGATGCTCTAGATGTGTGCGGCCGGACAGAAATCAGA
  AATTATCGGAGGGAGGTAGTAGAAGATATCAACAAATTATTGAAATATCTGGATTTGGAAGAGGAAGCAG
  ACACAACTAAAGCATTTGACCTGAGACAGAATCATTCCATTTTAAAAATAGAAAAGGTCCTCAAGAGAAT
  GAGAGAAATAAAAAATGAACTTCTCCAAGCACAAAACCCTTCTGAATTGTACCTGAGCTCCAAAACAGAA
  TTGCAGGGTTTAATTGGACAGTTGGATGAGGTAAGTCTTGAAAAAAACCCCTGCATCCGGGAAGCCAGGA
  GAAGAGCAGTGATCGAGGTGCAAACTCTGATCACATATATTGACTTGAAGGAGGCCCTTGAGAAAAGAAA
  GCTGTTTGCTTGTGAGGAGCACCCATCCCATAAAGCCGTCTGGAACGTCCTTGGAAACTTGTCTGAGATC
  CAGGGAGAAGTTCTTTCATTTGATGGAAATCGAACCGATAAGAACTACATCCGGCTGGAAGAGCTGCTCA
  CCAAGCAGCTGCTAGCCCTGGATGCTGTTGATCCGCAGGGAGAAGAGAAGTGTAAGGCTGCCAGGAAACA
  AGCTGTGAGGCTTGCGCAGAATATTCTCAGCTATCTCGACCTGAAATCTGATGAATGGGAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208123 representing NM_001015049
  Red=Cloning site Green=Tags(s)
  
  MRFHWLPTLSEPFDRNQELETCIRPLWTPSGSACETEHNKSMDMGNQHPSISRLQEIQKEVKSVEQQVIG
  FSGLSDDKNYKKLERILTKQLFEIDSVDTEGKGDIQQARKRAAQETERLLKELEQNANHPHRIEIQNIFE
  EAQSLVREKIVPFYNGGNCVTDEFEEGIQDIILRLTHVKTGGKISLRKARYHTLTKICAVQEIIEDCMKK
  QPSLPLSEDAHPSVAKINFVMCEVNKARGVLIALLMGVNNNETCRHLSCVLSGLIADLDALDVCGRTEIR
  NYRREVVEDINKLLKYLDLEEEADTTKAFDLRQNHSILKIEKVLKRMREIKNELLQAQNPSELYLSSKTE
  LQGLIGQLDEVSLEKNPCIREARRRAVIEVQTLITYIDLKEALEKRKLFACEEHPSHKAVWNVLGNLSEI
  QGEVLSFDGNRTDKNYIRLEELLTKQLLALDAVDPQGEEKCKAARKQAVRLAQNILSYLDLKSDEWEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001015049
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001015049.1, NM_001015049.2, NP_001015049.1
• RefSeq Size: 5073 bp
• RefSeq ORF: 1467 bp
• Locus ID: 9529
• Cytogenetics: 14q32.33
• Protein Families: Druggable Genome
• MW: 56 kDa
• Gene Summary: The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]