NUP153 (NM_005124) Human Tagged ORF Clone

CAT#: RC208264

NUP153 (Myc-DDK-tagged)-Human nucleoporin 153kDa (NUP153)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NUP153
• Synonyms: HNUP153; N153
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208264 representing NM_005124
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGGAGCCGGAGGAGTCGGAGGGGGCGGTGGCGGCAAGATCCGGACGCGGCGTTGCCACCAGG
  GGCCAATTAAGCCTTACCAGCAGGGGCGACAACAGCATCAGGGCATTCTTAGCAGGGTTACAGAATCTGT
  TAAGAATATTGTGCCAGGGTGGCTACAAAGATACTTCAACAAGAATGAAGATGTATGCAGCTGTTCAACA
  GACACAAGCGAGGTTCCACGCTGGCCAGAAAATAAAGAGGACCATCTGGTATATGCCGATGAGGAGAGCT
  CTAATATTACTGATGGGAGAATCACACCTGAGCCAGCAGTCAGTAATACAGAAGAACCTTCAACAACTAG
  TACTGCTTCAAATTATCCAGATGTGTTAACAAGGCCTTCTCTTCATCGGAGCCATCTGAATTTTTCCATG
  TTGGAATCCCCTGCATTACACTGTCAGCCATCTACATCCTCGGCATTCCCAATTGGCAGTTCGGGATTTT
  CCCTTGTAAAGGAAATTAAAGATTCTACCTCTCAGCATGATGATGATAACATCTCAACTACCAGTGGTTT
  TTCTTCAAGAGCTTCTGATAAAGATATAACTGTTTCAAAGAACACTTCATTGCCACCTCTGTGGTCCCCA
  GAAGCTGAACGTTCTCACTCACTCTCACAGCACACTGCCACCAGCTCAAAAAAACCAGCATTCAACTTGT
  CTGCCTTTGGAACACTTTCCCCTTCACTTGGGAATTCTTCAATCCTTAAAACCAGTCAGCTTGGAGATTC
  TCCTTTTTATCCTGGAAAAACAACATACGGTGGGGCAGCAGCTGCTGTAAGACAGTCTAAACTACGAAAT
  ACACCTTATCAGGCACCAGTTAGAAGACAAATGAAAGCTAAGCAACTCAGTGCACAATCTTACGGTGTGA
  CCAGTTCAACAGCTCGGCGAATATTGCAGTCTTTAGAGAAGATGTCAAGCCCTTTAGCGGATGCAAAAAG
  AATTCCATCCATTGTTTCTTCTCCTCTGAATTCTCCTCTTGATAGGAGTGGGATAGATATCACAGATTTT
  CAGGCCAAAAGAGAAAAGGTGGATTCTCAATATCCTCCTGTTCAGAGACTTATGACCCCAAAGCCAGTTT
  CCATAGCAACAAATCGAAGTGTTTATTTTAAACCATCTCTGACTCCTTCTGGTGAATTCAGGAAGACTAA
  TCAAAGAATAGATAACAAGTGCAGTACTGGATATGAAAAAAATATGACACCCGGACAAAATAGAGAACAA
  CGAGAAAGTGGCTTTTCATATCCAAATTTCAGTTTGCCTGCAGCCAATGGTTTATCTTCTGGAGTAGGTG
  GTGGAGGTGGCAAGATGAGACGAGAAAGAACACGCTTTGTTGCTTCTAAACCTCTGGAGGAGGAGGAAAT
  GGAAGTTCCAGTATTACCGAAAATCTCTCTACCGATCACCAGTTCTTCACTGCCTACCTTTAATTTTAGT
  TCCCCTGAGATCACAACTTCCTCTCCATCACCCATCAATTCGTCTCAAGCATTAACAAACAAGGTACAAA
  TGACCTCTCCGAGCAGCACTGGCAGTCCCATGTTTAAATTTTCATCTCCAATCGTAAAATCTACTGAGGC
  AAATGTACTACCTCCATCATCTATTGGATTTACATTTAGTGTGCCTGTTGCAAAAACAGCAGAACTTTCT
  GGTTCTAGTAGTACTTTAGAACCAATTATAAGTAGTTCAGCTCATCATGTCACTACAGTGAACAGTACAA
  ATTGTAAGAAGACACCACCTGAAGATTGTGAGGGTCCTTTTAGACCTGCAGAAATCCTGAAAGAAGGAAG
  TGTTCTAGATATTCTGAAAAGCCCTGGTTTCGCATCGCCGAAGATAGATTCTGTTGCTGCTCAGCCCACC
  GCAACAAGCCCAGTAGTTTATACAAGACCAGCAATAAGTAGCTTTTCTTCTAGTGGAATTGGGTTTGGGG
  AGAGTTTAAAAGCTGGGTCATCATGGCAGTGTGATACATGTCTACTCCAGAACAAAGTTACAGACAACAA
  ATGCATAGCCTGTCAAGCAGCAAAATTGTCACCCAGAGATACTGCTAAACAGACTGGAATTGAAACACCA
  AATAAAAGTGGCAAAACAACTCTTTCTGCATCAGGGACAGGCTTTGGAGACAAATTTAAACCAGTGATAG
  GCACTTGGGATTGTGATACCTGTTTAGTGCAAAATAAACCTGAAGCAATAAAATGTGTAGCCTGTGAAAC
  ACCGAAACCTGGAACTTGTGTGAAGCGAGCCCTTACATTGACAGTGGTTTCGGAAAGTGCTGAGACTATG
  ACTGCTTCATCTTCCAGCTGCACTGTAACCACTGGTACCTTAGGATTTGGAGATAAATTCAAAAGGCCCA
  TTGGATCTTGGGAGTGTTCAGTATGCTGTGTTTCTAATAATGCAGAAGACAATAAGTGTGTGTCCTGTAT
  GTCTGAGAAACCAGGAAGTTCAGTACCTGCTTCAAGTAGCAGCACTGTACCTGTCTCTCTGCCTTCTGGA
  GGCTCTCTAGGATTGGAAAAGTTCAAGAAACCCGAGGGAAGCTGGGACTGTGAATTGTGCCTAGTGCAGA
  ATAAGGCAGACTCTACCAAATGTTTGGCATGTGAAAGTGCAAAGCCAGGCACAAAATCTGGGTTTAAAGG
  CTTTGACACATCTTCCTCATCTTCGAACTCAGCAGCCTCCTCATCCTTCAAATTTGGTGTCTCATCATCC
  TCTTCTGGGCCTTCTCAGACTTTAACAAGCACTGGAAATTTTAAATTTGGAGATCAGGGAGGATTCAAAA
  TAGGTGTGTCATCTGATTCTGGGTCTATAAACCCCATGAGTGAAGGCTTTAAATTTTCTAAACCAATAGG
  AGATTTTAAATTTGGAGTTTCATCTGAATCTAAGCCCGAAGAAGTTAAAAAAGATAGTAAGAATGATAAT
  TTTAAGTTTGGACTTTCTTCTGGTTTAAGCAACCCAGTTTCTTTAACTCCATTTCAATTTGGGGTATCTA
  ATCTTGGACAGGAAGAAAAGAAAGAGGAACTGCCCAAATCTTCCTCTGCAGGTTTTAGCTTTGGTACAGG
  TGTTATTAACTCCACCCCTGCTCCTGCTAACACCATAGTGACCTCTGAGAACAAGAGCAGCTTCAACCTT
  GGAACCATAGAAACCAAGAGTGCTTCAGTGGCTCCTTTCACATGTAAGACATCAGAAGCTAAAAAAGAAG
  AAATGCCTGCCACCAAAGGAGGATTCTCTTTTGGCAACGTGGAGCCTGCCTCTCTGCCATCTGCCTCAGT
  GTTTGTTTTGGGAAGGACAGAAGAGAAACAGCAAGAGCCTGTCACTTCTACTTCCCTAGTTTTTGGGAAG
  AAAGCTGACAATGAAGAGCCAAAGTGTCAACCAGTGTTTTCCTTTGGGAATTCAGAGCAAACCAAAGATG
  AGAATTCTTCAAAGTCCACATTTAGTTTTAGTATGACAAAACCATCTGAGAAGGAATCTGAACAGCCAGC
  AAAAGCCACTTTTGCCTTTGGAGCTCAAACTAGTACTACAGCTGATCAAGGTGCAGCAAAGCCAGTTTTT
  AGTTTCTTGAACAACAGTTCCTCTAGTTCAAGTACACCAGCCACTTCTGCTGGTGGTGGCATATTTGGTA
  GTTCCACCTCTTCCTCCAATCCACCTGTGGCTACCTTTGTGTTTGGACAGTCCAGCAATCCTGTGAGCAG
  CTCTGCCTTTGGTAACACTGCTGAATCCAGCACCTCTCAGTCTTTGCTATTTTCTCAAGATAGCAAACTA
  GCAACCACATCCAGCACAGGTACAGCTGTCACCCCATTTGTCTTTGGTCCAGGAGCCAGCAGTAATAATA
  CTACCACCTCTGGTTTCGGCTTTGGAGCCACAACCACATCTAGCTCTGCAGGATCCTCCTTTGTATTTGG
  AACTGGACCCTCAGCACCATCTGCCAGTCCAGCATTTGGTGCTAACCAGACCCCAACATTTGGACAAAGT
  CAAGGTGCCAGCCAGCCCAATCCCCCAGGCTTTGGATCTATATCATCTTCCACAGCATTATTTCCCACTG
  GTTCTCAGCCTGCACCACCTACTTTTGGGACAGTGTCAAGCAGTAGCCAGCCCCCTGTGTTTGGACAGCA
  ACCTAGTCAGTCTGCATTTGGCTCTGGAACAACTCCTAATTCTAGTTCGGCTTTCCAGTTTGGCAGCAGC
  ACTACAAATTTCAACTTCACAAACAACAGTCCATCAGGAGTGTTCACATTTGGTGCAAATTCTAGCACAC
  CTGCAGCCTCAGCCCAGCCTTCAGGCTCGGGGGGCTTTCCATTTAACCAGTCTCCAGCAGCATTTACAGT
  GGGGTCAAATGGGAAAAATGTGTTCTCTTCTTCTGGAACTTCATTCTCTGGTCGCAAGATAAAGACTGCT
  GTTAGACGCAGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208264 representing NM_005124
  Red=Cloning site Green=Tags(s)
  
  MASGAGGVGGGGGGKIRTRRCHQGPIKPYQQGRQQHQGILSRVTESVKNIVPGWLQRYFNKNEDVCSCST
  DTSEVPRWPENKEDHLVYADEESSNITDGRITPEPAVSNTEEPSTTSTASNYPDVLTRPSLHRSHLNFSM
  LESPALHCQPSTSSAFPIGSSGFSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSLPPLWSP
  EAERSHSLSQHTATSSKKPAFNLSAFGTLSPSLGNSSILKTSQLGDSPFYPGKTTYGGAAAAVRQSKLRN
  TPYQAPVRRQMKAKQLSAQSYGVTSSTARRILQSLEKMSSPLADAKRIPSIVSSPLNSPLDRSGIDITDF
  QAKREKVDSQYPPVQRLMTPKPVSIATNRSVYFKPSLTPSGEFRKTNQRIDNKCSTGYEKNMTPGQNREQ
  RESGFSYPNFSLPAANGLSSGVGGGGGKMRRERTRFVASKPLEEEEMEVPVLPKISLPITSSSLPTFNFS
  SPEITTSSPSPINSSQALTNKVQMTSPSSTGSPMFKFSSPIVKSTEANVLPPSSIGFTFSVPVAKTAELS
  GSSSTLEPIISSSAHHVTTVNSTNCKKTPPEDCEGPFRPAEILKEGSVLDILKSPGFASPKIDSVAAQPT
  ATSPVVYTRPAISSFSSSGIGFGESLKAGSSWQCDTCLLQNKVTDNKCIACQAAKLSPRDTAKQTGIETP
  NKSGKTTLSASGTGFGDKFKPVIGTWDCDTCLVQNKPEAIKCVACETPKPGTCVKRALTLTVVSESAETM
  TASSSSCTVTTGTLGFGDKFKRPIGSWECSVCCVSNNAEDNKCVSCMSEKPGSSVPASSSSTVPVSLPSG
  GSLGLEKFKKPEGSWDCELCLVQNKADSTKCLACESAKPGTKSGFKGFDTSSSSSNSAASSSFKFGVSSS
  SSGPSQTLTSTGNFKFGDQGGFKIGVSSDSGSINPMSEGFKFSKPIGDFKFGVSSESKPEEVKKDSKNDN
  FKFGLSSGLSNPVSLTPFQFGVSNLGQEEKKEELPKSSSAGFSFGTGVINSTPAPANTIVTSENKSSFNL
  GTIETKSASVAPFTCKTSEAKKEEMPATKGGFSFGNVEPASLPSASVFVLGRTEEKQQEPVTSTSLVFGK
  KADNEEPKCQPVFSFGNSEQTKDENSSKSTFSFSMTKPSEKESEQPAKATFAFGAQTSTTADQGAAKPVF
  SFLNNSSSSSSTPATSAGGGIFGSSTSSSNPPVATFVFGQSSNPVSSSAFGNTAESSTSQSLLFSQDSKL
  ATTSSTGTAVTPFVFGPGASSNNTTTSGFGFGATTTSSSAGSSFVFGTGPSAPSASPAFGANQTPTFGQS
  QGASQPNPPGFGSISSSTALFPTGSQPAPPTFGTVSSSSQPPVFGQQPSQSAFGSGTTPNSSSAFQFGSS
  TTNFNFTNNSPSGVFTFGANSSTPAASAQPSGSGGFPFNQSPAAFTVGSNGKNVFSSSGTSFSGRKIKTA
  VRRRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005124
• ORF Size: 4425 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005124.1, NM_005124.2, NM_005124.3, NP_005115.2
• RefSeq Size: 5687
• RefSeq ORF: 4428
• Locus ID: 9972
• Domains: zf-RanBP
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 153.8 kDa
• Gene Summary: Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]