PPP2R2A (NM_002717) Human Tagged ORF Clone

CAT#: RC208340

PPP2R2A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, alpha (PPP2R2A), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY400958) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208340 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP2R2A
• Synonyms: B55A; B55ALPHA; PR52A; PR55A; PR55alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208340 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGAGCTGGAGGAGGGAATGATATTCAGTGGTGTTTTTCTCAGGTGAAAGGAGCAGTAGATGATG
  ATGTAGCAGAAGCAGATATAATTTCTACAGTAGAATTTAATCATTCTGGAGAATTACTAGCAACAGGAGA
  TAAAGGTGGTAGAGTTGTCATCTTTCAACAGGAGCAGGAGAACAAAATCCAGTCTCATAGCAGAGGAGAA
  TATAATGTTTACAGCACCTTCCAGAGCCATGAACCAGAGTTTGACTACTTGAAAAGTTTAGAAATAGAAG
  AAAAGATCAACAAAATTAGGTGGTTACCCCAGAAAAATGCTGCTCAGTTTTTATTGTCTACCAATGATAA
  AACAATAAAATTATGGAAAATCAGTGAAAGGGACAAAAGACCAGAAGGGTATAACTTGAAAGAGGAGGAT
  GGAAGGTATAGAGATCCTACTACAGTTACTACACTACGAGTGCCAGTCTTTAGGCCTATGGATCTAATGG
  TTGAGGCCAGTCCACGAAGAATATTTGCCAATGCTCATACATATCACATCAACTCAATTTCTATTAATAG
  TGATTATGAAACATATTTATCTGCAGATGATTTGCGGATTAATCTTTGGCATCTGGAAATTACAGACAGG
  AGTTTTAACATTGTGGATATCAAGCCTGCCAATATGGAAGAGCTAACAGAGGTGATTACAGCAGCAGAAT
  TTCATCCAAACAGCTGTAACACATTTGTATACAGCAGCAGTAAAGGAACTATTCGGCTATGTGACATGAG
  GGCATCTGCCCTCTGTGATAGACATTCTAAATTGTTTGAAGAACCTGAAGATCCCAGTAACAGGTCATTT
  TTTTCCGAAATCATCTCCTCTATTTCGGATGTAAAATTCAGCCATAGTGGTCGATATATGATGACTAGAG
  ACTATTTGTCAGTCAAAATTTGGGACTTAAATATGGAAAACAGGCCTGTGGAAACATACCAGGTGCATGA
  ATACCTCAGAAGTAAACTCTGTTCACTGTATGAAAATGACTGCATATTTGACAAATTTGAATGTTGTTGG
  AATGGATCTGACAGTGTTGTCATGACTGGATCTTACAATAATTTCTTCAGAATGTTTGACAGAAACACAA
  AGCGAGACATAACCCTAGAAGCATCGCGGGAAAACAATAAGCCTCGCACAGTTCTGAAGCCTCGCAAAGT
  CTGTGCAAGTGGCAAGCGAAAGAAAGATGAAATAAGTGTTGACAGCCTAGACTTCAATAAGAAAATCCTT
  CACACAGCCTGGCACCCCAAGGAAAATATCATTGCCGTAGCTACTACAAACAATCTGTATATATTTCAAG
  ACAAAGTGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208340 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGAGGGNDIQWCFSQVKGAVDDDVAEADIISTVEFNHSGELLATGDKGGRVVIFQQEQENKIQSHSRGE
  YNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQKNAAQFLLSTNDKTIKLWKISERDKRPEGYNLKEED
  GRYRDPTTVTTLRVPVFRPMDLMVEASPRRIFANAHTYHINSISINSDYETYLSADDLRINLWHLEITDR
  SFNIVDIKPANMEELTEVITAAEFHPNSCNTFVYSSSKGTIRLCDMRASALCDRHSKLFEEPEDPSNRSF
  FSEIISSISDVKFSHSGRYMMTRDYLSVKIWDLNMENRPVETYQVHEYLRSKLCSLYENDCIFDKFECCW
  NGSDSVVMTGSYNNFFRMFDRNTKRDITLEASRENNKPRTVLKPRKVCASGKRKKDEISVDSLDFNKKIL
  HTAWHPKENIIAVATTNNLYIFQDKVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002717
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002717.1, NM_002717.2, NM_002717.3, NP_002708.1
• RefSeq Size: 3945 bp
• RefSeq ORF: 1344 bp
• Locus ID: 5520
• Cytogenetics: 8p21.2
• Domains: WD40
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Tight junction
• MW: 51.7 kDa
• Gene Summary: 'The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]'