PPP2R2A (NM_002717) Human Untagged Clone

CAT#: SC319956

PPP2R2A (untagged)-Human protein phosphatase 2, regulatory subunit B, alpha (PPP2R2A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R2A
• Synonyms: B55A; B55ALPHA; PR52A; PR55A; PR55alpha
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002717.2 CCTGCCCCTGCCGCTGCCGCCGCCGCCGTCGCTGTCGTAGTCGCCGCCGCCGCTGCCGGA
  GAAAGAGCACGAGCGGGGAAGCCCCAGAGTGAAATCTAGCATCCTGCCGGCTGGTCTGCC
  CGCCCCTCCTTCCTTTTCCCCCCGGCCCCCGTCCCCTCCCCCCGCAGGTGCCATCCGCCG
  CCATCCGCCCTCTCTACCCCCCCATCCCCAGGTGAGGGGGGTGAGTTCAGGAAGCGGAGA
  CCCCGAGGAACCCAGCAGGGTCACCATTTGCAGCGCAACATGGCAGGAGCTGGAGGAGGG
  AATGATATTCAGTGGTGTTTTTCTCAGGTGAAAGGAGCAGTAGATGATGATGTAGCAGAA
  GCAGATATAATTTCTACAGTAGAATTTAATCATTCTGGAGAATTACTAGCAACAGGAGAT
  AAAGGTGGTAGAGTTGTCATCTTTCAACAGGAGCAGGAGAACAAAATCCAGTCTCATAGC
  AGAGGAGAATATAATGTTTACAGCACCTTCCAGAGCCATGAACCAGAGTTTGACTACTTG
  AAAAGTTTAGAAATAGAAGAAAAGATCAACAAAATTAGGTGGTTACCCCAGAAAAATGCT
  GCTCAGTTTTTATTGTCTACCAATGATAAAACAATAAAATTATGGAAAATCAGTGAAAGG
  GACAAAAGACCAGAAGGGTATAACTTGAAAGAGGAGGATGGAAGGTATAGAGATCCTACT
  ACAGTTACTACACTACGAGTGCCAGTCTTTAGGCCTATGGATCTAATGGTTGAGGCCAGT
  CCACGAAGAATATTTGCCAATGCTCATACATATCACATCAACTCAATTTCTATTAATAGT
  GATTATGAAACATATTTATCTGCAGATGATTTGCGGATTAATCTTTGGCATCTGGAAATT
  ACAGACAGGAGTTTTAACATTGTGGATATCAAGCCTGCCAATATGGAAGAGCTAACAGAG
  GTGATTACAGCAGCAGAATTTCATCCAAACAGCTGTAACACATTTGTATACAGCAGCAGT
  AAAGGAACTATTCGGCTATGTGACATGAGGGCATCTGCCCTCTGTGATAGACATTCTAAA
  TTGTTTGAAGAACCTGAAGATCCCAGTAACAGGTCATTTTTTTCCGAAATCATCTCCTCT
  ATTTCGGATGTAAAATTCAGCCATAGTGGTCGATATATGATGACTAGAGACTATTTGTCA
  GTCAAAATTTGGGACTTAAATATGGAAAACAGGCCTGTGGAAACATACCAGGTGCATGAA
  TACCTCAGAAGTAAACTCTGTTCACTGTATGAAAATGACTGCATATTTGACAAATTTGAA
  TGTTGTTGGAATGGATCTGACAGTGTTGTCATGACTGGATCTTACAATAATTTCTTCAGA
  ATGTTTGACAGAAACACAAAGCGAGACATAACCCTAGAAGCATCGCGGGAAAACAATAAG
  CCTCGCACAGTTCTGAAGCCTCGCAAAGTCTGTGCAAGTGGCAAGCGAAAGAAAGATGAA
  ATAAGTGTTGACAGCCTAGACTTCAATAAGAAAATCCTTCACACAGCCTGGCACCCCAAG
  GAAAATATCATTGCCGTAGCTACTACAAACAATCTGTATATATTTCAAGACAAAGTGAAT
  TAGGGTTGGCATTCCTAGCAGAAGAACCCACTTCCTGCTTAGTTGAGATAGTTGAATCTA
  GCATTCGTTCCTATAAAAGAGAGAGGTCCATTGTGGCGCCCCTTTCCAGTGTTTGACAGT
  GTGCCATTCGACAACACATTGTTATAGCTACATGGAGAAAGCTCTGTGGATTCATCACTG
  TGGTGTTCTCCATGTCTGCTAGCCATTTAGGTAAGGGTAGGGCACTTTTAATTTAAATGA
  CTTCTTGCACCATCTTGCCTAATGGACTAGATTGGACTGTATCAACATTGATTTACTCCA
  CTTTTTATGCCTTCCATTGTGATGACGTCAAACACAGTGAAAGCCTTCAGTCATGCTATG
  GGATTTAATTGTGTATCCTCATTACTGTATCATTTGTGGGGTACACCCCTTCCCCCTTTT
  TTTAAATTAAATACAGCTCATTCTTACTGTGGCTTGTAGCATTCCTCCTCTTCTGGCCTC
  CTGGACTGCTCCCCTTCATCTCTTACCCTTGCCCCCTCCACCCGGTCTTGGTGGTGGTAT
  ATTAAAAAAAGAAAGAATGAAAGCACACAAAATGAGTCAGTTTGGGGTCAGTGGTATAAA
  GGGGGTATATGTTGCAAACAAATGTTTTAGTAACAGTTGGCTGTAATCACTCCTCGCCGT
  GTCTGGCACTGAAAATAAGGAAAAAAAACCTACTACTGAATAAAAGTGACAAAGAATGGA
  AAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002717
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002717.2, NP_002708.1
• RefSeq Size: 2385 bp
• RefSeq ORF: 1344 bp
• Locus ID: 5520
• Cytogenetics: 8p21.2
• Domains: WD40
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Tight junction
• Gene Summary: 'The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.