ALOX12B (NM_001139) Human Tagged ORF Clone

CAT#: RC208343

ALOX12B (Myc-DDK-tagged)-Human arachidonate 12-lipoxygenase, 12R type (ALOX12B)

Product Images

Western blot validation of overexpression lysate (Cat# LY420101) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208343 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALOX12B
• Synonyms: 12R-LOX; ARCI2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208343 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCTACAAAGTCAGGGTGGCCACAGGCACCGACCTCTTGTCGGGAACACGGGACTCCATCTCAC
  TGACCATTGTGGGGACACAAGGAGAGAGCCATAAGCAGCTGCTGAACCACTTTGGGAGAGACTTTGCAAC
  TGGGGCGGTGGGCCAGTACACCGTGCAGTGCCCTCAGGACCTGGGTGAGCTCATCATCATCCGCCTGCAC
  AAAGAGCGGTACGCCTTCTTCCCCAAGGACCCTTGGTACTGCAACTATGTGCAGATCTGTGCCCCCAACG
  GCCGTATCTACCACTTCCCCGCCTACCAGTGGATGGATGGCTACGAGACCCTGGCACTCCGGGAGGCCAC
  AGGAAAGACAACAGCAGATGACTCGCTCCCCGTCCTCCTGGAGCACAGAAAAGAGGAGATCAGAGCCAAG
  CAGGACTTCTACCACTGGCGAGTCTTTCTTCCTGGCCTGCCCAGCTATGTGCACATTCCCAGTTACCGCC
  CTCCGGTGCGGAGGCATCGCAACCCCAACCGGCCTGAGTGGAATGGCTATATTCCGGGATTCCCAATTCT
  CATCAACTTTAAGGCCACCAAGTTCCTGAACTTAAATCTCCGCTACTCCTTCCTCAAGACGGCCTCCTTC
  TTCGTCCGCCTGGGGCCCATGGCACTGGCTTTCAAAGTCCGCGGCCTGTTGGACTGCAAACATTCGTGGA
  AGAGGCTGAAGGACATTAGGAAAATTTTCCCTGGCAAGAAATCTGTCGTCTCCGAGTACGTGGCCGAGCA
  CTGGGCAGAGGACACCTTCTTTGGGTACCAGTACCTCAACGGCGTCAACCCCGGCCTGATCCGCCGCTGC
  ACGCGGATCCCAGACAAGTTCCCCGTCACAGACGACATGGTGGCTCCGTTCCTGGGCGAGGGAACGTGCT
  TGCAAGCGGAGCTGGAGAAGGGGAACATTTACCTGGCCGACTACCGCATCATGGAGGGCATCCCCACCGT
  GGAGCTCAGCGGCCGGAAGCAGCACCACTGCGCCCCCCTCTGCCTGCTGCACTTTGGACCCGAGGGCAAG
  ATGATGCCCATCGCCATCCAGCTCAGCCAGACCCCTGGGCCAGATTGCCCCATCTTCCTGCCCAGTGATT
  CTGAGTGGGACTGGCTGCTAGCCAAGACGTGGGTACGCTATGCGGAGTTCTACAGCCACGAGGCCATCGC
  CCACCTGCTGGAGACACACCTCATTGCTGAGGCCTTCTGCCTGGCCTTGCTGAGGAACCTGCCCATGTGC
  CACCCCCTCTACAAGCTCCTCATCCCCCATACCCGATACACCGTCCAGATCAACAGCATTGGCCGGGCCG
  TTCTCCTCAATGAGGGGGGGCTCTCTGCCAAGGGCATGTCCCTGGGCGTGGAAGGCTTTGCTGGGGTGAT
  GGTACGGGCTCTGTCGGAGCTCACCTATGACAGCCTCTACCTCCCCAATGACTTTGTGGAGCGTGGGGTC
  CAGGACCTGCCTGGATATTACTACCGCGATGACAGCTTGGCGGTGTGGAATGCACTGGAGAAGTATGTGA
  CGGAGATCATCACCTATTATTACCCGAGTGACGCAGCCGTGGAGGGTGATCCGGAATTGCAGTCTTGGGT
  GCAGGAAATATTTAAAGAGTGCCTCCTGGGGCGGGAGAGCTCAGGCTTCCCTAGGTGCTTGCGAACCGTG
  CCTGAGCTGATCCGATATGTCACTATAGTCATCTACACCTGCTCTGCCAAGCACGCTGCTGTCAACACAG
  GCCAGATGGAGTTCACCGCCTGGATGCCCAACTTCCCAGCGTCCATGCGGAATCCACCGATTCAGACTAA
  GGGGCTGACCACTCTGGAGACCTTCATGGACACGTTGCCGGATGTGAAGACCACGTGCATCACGCTGCTG
  GTGCTCTGGACCCTCAGCCGAGAGCCTGACGACAGGCGGCCCCTGGGACACTTCCCGGACATTCACTTCG
  TGGAGGAGGCCCCGCGGAGGAGCATAGAGGCGTTCCGCCAGCGCCTGAACCAGATCTCACACGACATCCG
  CCAGCGCAACAAGTGCCTTCCCATCCCCTACTACTACCTGGACCCGGTGCTGATTGAGAACAGCATTTCT
  ATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208343 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATYKVRVATGTDLLSGTRDSISLTIVGTQGESHKQLLNHFGRDFATGAVGQYTVQCPQDLGELIIIRLH
  KERYAFFPKDPWYCNYVQICAPNGRIYHFPAYQWMDGYETLALREATGKTTADDSLPVLLEHRKEEIRAK
  QDFYHWRVFLPGLPSYVHIPSYRPPVRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNLRYSFLKTASF
  FVRLGPMALAFKVRGLLDCKHSWKRLKDIRKIFPGKKSVVSEYVAEHWAEDTFFGYQYLNGVNPGLIRRC
  TRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGK
  MMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMC
  HPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGV
  QDLPGYYYRDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRESSGFPRCLRTV
  PELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLL
  VLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYYLDPVLIENSIS
  I
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001139
• ORF Size: 2103 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001139.1, NM_001139.2, NP_001130.1
• RefSeq Size: 2502 bp
• RefSeq ORF: 2106 bp
• Locus ID: 242
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• MW: 80.4 kDa
• Gene Summary: 'This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma. [provided by RefSeq, Sep 2015]'