ALOX12B (NM_001139) Human Untagged Clone

CAT#: SC126150

ALOX12B (untagged)-Human arachidonate 12-lipoxygenase, 12R type (ALOX12B)


  "NM_001139" in other vectors (4)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ALOX12B
Synonyms 12R-LOX; ARCI2
Vector PCMV6-Neo
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_001139 edited
GGCCTGGGTGGCGAATTCGGCACGAGGGCCCAGACACCTGCTCACTCACCACCAGCTGGG
CTCCGCCTGGGCCTGCCCGGCACCCACCCCGGCCACCAAGGGCAGCAGCTTTTCCAGAAT
TTGGCTGGCAGGCCCAGTCACCCCACCTCGCCACCTCACCACTGCACCTCGGAGGCCAGC
CCTGTCCACTCCACTCTGTGCCTGGCTTCTCTTGCCTGCCTTGGGCCTTCGTGTGGCCCC
TCCACGGTGTCTGGGACTGAGTGCCCCTCTTGCCTCCTGAAGAGCAGCCATGGCCACCTA
CAAAGTCAGGGTGGCCACAGGCACCGACCTCTTGTCGGGAACACGGGACTCCATCTCACT
GACCATTGTGGGGACACAAGGAGAGAGCCATAAGCAGCTGCTGAACCACTTTGGGAGAGA
CTTTGCAACTGGGGCGGTGGGCCAGTACACCGTGCAGTGCCCTCAGGACCTGGGTGAGCT
CATCATCATCCGCCTGCACAAAGAGCGGTACGCCTTCTTCCCCAAGGACCCTTGGTACTG
CAACTATGTGCAGATCTGTGCCCCCAACGGCCGTATCTACCACTTCCCCGCCTACCAGTG
GATGGATGGCTACGAGACCCTGGCACTCCGGGAGGCCACAGGAAAGACAACAGCAGATGA
CTCGCTCCCCGTCCTCCTGGAGCACAGAAAAGAGGAGATCAGAGCCAAGCAGGACTTCTA
CCACTGGCGAGTCTTTCTTCCTGGCCTGCCCAGCTATGTGCACATTCCCAGTTACCGCCC
TCCGGTGCGGAGGCATCGCAACCCCAACCGGCCTGAGTGGAATGGCTATATTCCGGGATT
CCCAATTCTCATCAACTTTAAGGCCACCAAGTTCCTGAACTTAAATCTCCGCTACTCCTT
CCTCAAGACGGCCTCCTTCTTCGTCCGCCTGGGGCCCATGGCACTGGCTTTCAAAGTCCG
CGGCCTGTTGGACTGCAAACATTCGTGGAAGAGGCTGAAGGACATTAGGAAAATTTTCCC
TGGCAAGAAATCTGTCGTCTCCGAGTACGTGGCCGAGCACTGGGCAGAGGACACCTTCTT
TGGGTACCAGTACCTCAACGGCGTCAACCCCGGCCTGATCCGCCGCTGCACGCGGATCCC
AGACAAGTTCCCCGTCACAGACGACATGGTGGCTCCGTTCCTGGGCGAGGGAACGTGCTT
GCAAGCGGAGCTGGAGAAGGGGAACATTTACCTGGCCGACTACCGCATCATGGAGGGCAT
CCCCACCGTGGAGCTCAGCGGCCGGAAGCAGCACCACTGCGCCCCCCTCTGCCTGCTGCA
CTTTGGACCCGAGGGCAAGATGATGCCCATCGCCATCCAGCTCAGCCAGACCCCTGGGCC
AGATTGCCCCATCTTCCTGCCCAGTGATTCTGAGTGGGACTGGCTGCTAGCCAAGACGTG
GGTACGCTATGCGGAGTTCTACAGCCACGAGGCCATCGCCCACCTGCTGGAGACACACCT
CATTGCTGAGGCCTTCTGCCTGGCCTTGCTGAGGAACCTGCCCATGTGCCACCCCCTCTA
CAAGCTCCTCATCCCCCATACCCGATACACCGTCCAGATCAACAGCATTGGCCGGGCCGT
TCTCCTCAATGAGGGGGGGCTCTCTGCCAAGGGCATGTCCCTGGGCGTGGAAGGCTTTGC
TGGGGTGATGGTACGGGCTCTGTCGGAGCTCACCTATGACAGCCTCTACCTCCCCAATGA
CTTTGTGGAGCGTGGGGTCCAGGACCTGCCTGGATATTACTACCGCGATGACAGCTTGGC
GGTGTGGAATGCACTGGAGAAGTATGTGACGGAGATCATCACCTATTATTACCCGAGTGA
CGCAGCCGTGGAGGGTGATCCGGAATTGCAGTCTTGGGTGCAGGAAATATTTAAAGAGTG
CCTCCTGGGGCGGGAGAGCTCAGGCTTCCCTAGGTGCTTGCGAACCGTGCCTGAGCTGAT
CCGATATGTCACTATAGTCATCTACACCTGCTCTGCCAAGCACGCTGCTGTCAACACAGG
CCAGATGGAGTTCACCGCCTGGATGCCCAACTTCCCAGCGTCCATGCGGAATCCACCGAT
TCAGACTAAGGGGCTGACCACTCTGGAGACCTTCATGGACACGTTGCCGGATGTGAAGAC
CACGTGCATCACGCTGCTGGTGCTCTGGACCCTCAGCCGAGAGCCTGACGACAGGCGGCC
CCTGGGACACTTCCCGGACATTCACTTCGTGGAGGAGGCCCCGCGGAGGAGCATAGAGGC
GTTCCGCCAGCGCCTGAACCAGATCTCACACGACATCCGCCAGCGCAACAAGTGCCTTCC
CATCCCCTACTACTACCTGGACCCGGTGCTGATTGAGAACAGCATTTCTATTTAGGAGCG
CGCTTCCCGTCTCTCCTCTCCCCATTCTGTGCCCTACTATTTTCAACAAAACAAAACAAA
CAAGCAAAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_001139 unedited
NCCGTCAAATATTGTAAACGACTCACTATAGGCGGCCGCGNAATTCGGCACGAGGGCCCA
AACACCTGCTCACTCACCACCAGCTGGGCTCCGCCTGGGCCTGCCCGGCACCCACCCCGG
CCACCAAGGGCAGCAGCTTTTCCAGAATTTGGCTGGCAGGCCCAGTCACCCCACCTCGCC
ACCTCACCACTGCACCTCGGAGGCCAGCCCTGTCCACTCCACTCTGTGCCTGGCTTCTCT
TGCCTGCCTTGGGCCTTCGTGTGGCCCCTCCACGGTGTCTGGGACTGAGTGCCCCTCTTG
CCTCCTGAAGAGCAGCCATGGCCACCTACAAAGTCAGGGTGGCCACAGGCACCGACCTCT
TGTCGGGAACACGGGACTCCATCTCACTGACCATTGTGGGGACACAAGGAGAGAGCCATA
AGCAGCTGCTGAACCACTTTGGGAGAGACTTTGCAACTGGGGCGGTGGGCCAGTACACCG
TGCAGTGCCCTCAGGACCTGNGTGAGCTCATCATCATCCGCCTGCACAAAGAGCGGTACG
CCTTCTTCCCCAAGGACCCTTGGTACTGCAACTATGTGCAGATCTGTGCCCCCAACGGCC
GTATCTACCACTTCCCCGCCTACCAGTGGATGGATGGCTACGAGACCCTGGCACTCCGGG
AGGCCACAGGAAAGACAACAGCAGATGACTCGCTCCCCGTCCTCCTGGAGCACAGAAAAG
AGGAGATCAGAGCCAAGCAGGACTTCTACCACTGGCGAGTCTTTCTTCCTGGCCTGCCCA
GCTATGTGCACATTCCCAGTTACCGCCCTCCGGGTGGCGAGCATCGCAACCCCAACCGGC
CTGAGTGGAATGGCTATATTCCGGGGATTCCCATTCTCATCAACTTTAAGCCACCAAGTT
CCTGAACTAAATCTNCGCTACTCTTNCTCAAGACGGCCTCTT
Restriction Sites Please inquire     
ACCN NM_001139
Insert Size 2500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001139.1.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001139.1, NP_001130.1
RefSeq Size 2469 bp
RefSeq ORF 2106 bp
Locus ID 242
Cytogenetics 17p13.1
Protein Families Druggable Genome
Protein Pathways Arachidonic acid metabolism, Metabolic pathways
Gene Summary 'This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma. [provided by RefSeq, Sep 2015]'

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