ALOX12B (NM_001139) Human Untagged Clone

CAT#: SC126150

ALOX12B (untagged)-Human arachidonate 12-lipoxygenase, 12R type (ALOX12B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALOX12B
• Synonyms: 12R-LOX; ARCI2
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001139 edited
  GGCCTGGGTGGCGAATTCGGCACGAGGGCCCAGACACCTGCTCACTCACCACCAGCTGGG
  CTCCGCCTGGGCCTGCCCGGCACCCACCCCGGCCACCAAGGGCAGCAGCTTTTCCAGAAT
  TTGGCTGGCAGGCCCAGTCACCCCACCTCGCCACCTCACCACTGCACCTCGGAGGCCAGC
  CCTGTCCACTCCACTCTGTGCCTGGCTTCTCTTGCCTGCCTTGGGCCTTCGTGTGGCCCC
  TCCACGGTGTCTGGGACTGAGTGCCCCTCTTGCCTCCTGAAGAGCAGCCATGGCCACCTA
  CAAAGTCAGGGTGGCCACAGGCACCGACCTCTTGTCGGGAACACGGGACTCCATCTCACT
  GACCATTGTGGGGACACAAGGAGAGAGCCATAAGCAGCTGCTGAACCACTTTGGGAGAGA
  CTTTGCAACTGGGGCGGTGGGCCAGTACACCGTGCAGTGCCCTCAGGACCTGGGTGAGCT
  CATCATCATCCGCCTGCACAAAGAGCGGTACGCCTTCTTCCCCAAGGACCCTTGGTACTG
  CAACTATGTGCAGATCTGTGCCCCCAACGGCCGTATCTACCACTTCCCCGCCTACCAGTG
  GATGGATGGCTACGAGACCCTGGCACTCCGGGAGGCCACAGGAAAGACAACAGCAGATGA
  CTCGCTCCCCGTCCTCCTGGAGCACAGAAAAGAGGAGATCAGAGCCAAGCAGGACTTCTA
  CCACTGGCGAGTCTTTCTTCCTGGCCTGCCCAGCTATGTGCACATTCCCAGTTACCGCCC
  TCCGGTGCGGAGGCATCGCAACCCCAACCGGCCTGAGTGGAATGGCTATATTCCGGGATT
  CCCAATTCTCATCAACTTTAAGGCCACCAAGTTCCTGAACTTAAATCTCCGCTACTCCTT
  CCTCAAGACGGCCTCCTTCTTCGTCCGCCTGGGGCCCATGGCACTGGCTTTCAAAGTCCG
  CGGCCTGTTGGACTGCAAACATTCGTGGAAGAGGCTGAAGGACATTAGGAAAATTTTCCC
  TGGCAAGAAATCTGTCGTCTCCGAGTACGTGGCCGAGCACTGGGCAGAGGACACCTTCTT
  TGGGTACCAGTACCTCAACGGCGTCAACCCCGGCCTGATCCGCCGCTGCACGCGGATCCC
  AGACAAGTTCCCCGTCACAGACGACATGGTGGCTCCGTTCCTGGGCGAGGGAACGTGCTT
  GCAAGCGGAGCTGGAGAAGGGGAACATTTACCTGGCCGACTACCGCATCATGGAGGGCAT
  CCCCACCGTGGAGCTCAGCGGCCGGAAGCAGCACCACTGCGCCCCCCTCTGCCTGCTGCA
  CTTTGGACCCGAGGGCAAGATGATGCCCATCGCCATCCAGCTCAGCCAGACCCCTGGGCC
  AGATTGCCCCATCTTCCTGCCCAGTGATTCTGAGTGGGACTGGCTGCTAGCCAAGACGTG
  GGTACGCTATGCGGAGTTCTACAGCCACGAGGCCATCGCCCACCTGCTGGAGACACACCT
  CATTGCTGAGGCCTTCTGCCTGGCCTTGCTGAGGAACCTGCCCATGTGCCACCCCCTCTA
  CAAGCTCCTCATCCCCCATACCCGATACACCGTCCAGATCAACAGCATTGGCCGGGCCGT
  TCTCCTCAATGAGGGGGGGCTCTCTGCCAAGGGCATGTCCCTGGGCGTGGAAGGCTTTGC
  TGGGGTGATGGTACGGGCTCTGTCGGAGCTCACCTATGACAGCCTCTACCTCCCCAATGA
  CTTTGTGGAGCGTGGGGTCCAGGACCTGCCTGGATATTACTACCGCGATGACAGCTTGGC
  GGTGTGGAATGCACTGGAGAAGTATGTGACGGAGATCATCACCTATTATTACCCGAGTGA
  CGCAGCCGTGGAGGGTGATCCGGAATTGCAGTCTTGGGTGCAGGAAATATTTAAAGAGTG
  CCTCCTGGGGCGGGAGAGCTCAGGCTTCCCTAGGTGCTTGCGAACCGTGCCTGAGCTGAT
  CCGATATGTCACTATAGTCATCTACACCTGCTCTGCCAAGCACGCTGCTGTCAACACAGG
  CCAGATGGAGTTCACCGCCTGGATGCCCAACTTCCCAGCGTCCATGCGGAATCCACCGAT
  TCAGACTAAGGGGCTGACCACTCTGGAGACCTTCATGGACACGTTGCCGGATGTGAAGAC
  CACGTGCATCACGCTGCTGGTGCTCTGGACCCTCAGCCGAGAGCCTGACGACAGGCGGCC
  CCTGGGACACTTCCCGGACATTCACTTCGTGGAGGAGGCCCCGCGGAGGAGCATAGAGGC
  GTTCCGCCAGCGCCTGAACCAGATCTCACACGACATCCGCCAGCGCAACAAGTGCCTTCC
  CATCCCCTACTACTACCTGGACCCGGTGCTGATTGAGAACAGCATTTCTATTTAGGAGCG
  CGCTTCCCGTCTCTCCTCTCCCCATTCTGTGCCCTACTATTTTCAACAAAACAAAACAAA
  CAAGCAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001139 unedited
  NCCGTCAAATATTGTAAACGACTCACTATAGGCGGCCGCGNAATTCGGCACGAGGGCCCA
  AACACCTGCTCACTCACCACCAGCTGGGCTCCGCCTGGGCCTGCCCGGCACCCACCCCGG
  CCACCAAGGGCAGCAGCTTTTCCAGAATTTGGCTGGCAGGCCCAGTCACCCCACCTCGCC
  ACCTCACCACTGCACCTCGGAGGCCAGCCCTGTCCACTCCACTCTGTGCCTGGCTTCTCT
  TGCCTGCCTTGGGCCTTCGTGTGGCCCCTCCACGGTGTCTGGGACTGAGTGCCCCTCTTG
  CCTCCTGAAGAGCAGCCATGGCCACCTACAAAGTCAGGGTGGCCACAGGCACCGACCTCT
  TGTCGGGAACACGGGACTCCATCTCACTGACCATTGTGGGGACACAAGGAGAGAGCCATA
  AGCAGCTGCTGAACCACTTTGGGAGAGACTTTGCAACTGGGGCGGTGGGCCAGTACACCG
  TGCAGTGCCCTCAGGACCTGNGTGAGCTCATCATCATCCGCCTGCACAAAGAGCGGTACG
  CCTTCTTCCCCAAGGACCCTTGGTACTGCAACTATGTGCAGATCTGTGCCCCCAACGGCC
  GTATCTACCACTTCCCCGCCTACCAGTGGATGGATGGCTACGAGACCCTGGCACTCCGGG
  AGGCCACAGGAAAGACAACAGCAGATGACTCGCTCCCCGTCCTCCTGGAGCACAGAAAAG
  AGGAGATCAGAGCCAAGCAGGACTTCTACCACTGGCGAGTCTTTCTTCCTGGCCTGCCCA
  GCTATGTGCACATTCCCAGTTACCGCCCTCCGGGTGGCGAGCATCGCAACCCCAACCGGC
  CTGAGTGGAATGGCTATATTCCGGGGATTCCCATTCTCATCAACTTTAAGCCACCAAGTT
  CCTGAACTAAATCTNCGCTACTCTTNCTCAAGACGGCCTCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_001139
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001139.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001139.1, NP_001130.1
• RefSeq Size: 2469 bp
• RefSeq ORF: 2106 bp
• Locus ID: 242
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma. [provided by RefSeq, Sep 2015]'