Axin 1 (AXIN1) (NM_181050) Human Tagged ORF Clone

CAT#: RC208349

AXIN1 (Myc-DDK-tagged)-Human axin 1 (AXIN1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405798) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208349 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AXIN1 protein (Cat# TP308349). The protein was produced from HEK293T cells transfected with AXIN1 cDNA clone (Cat# RC208349) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AXIN1
• Synonyms: AXIN; PPP1R49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208349 representing NM_181050
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATATCCAAGAGCAGGGTTTCCCCTTGGACCTCGGAGCAAGTTTCACCGAAGATGCTCCCCGACCCC
  CAGTGCCTGGTGAGGAGGGAGAACTGGTGTCCACAGACCCGAGGCCCGCCAGCTACAGTTTCTGCTCCGG
  GAAAGGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGATCTGGACCTGGGGTAT
  GAGCCTGAGGGCAGTGCCTCCCCCACCCCACCATACTTGAAGTGGGCTGAGTCACTGCATTCCCTGCTGG
  ATGACCAAGATGGGATAAGCCTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGTGCCGACTTGCTGGACTT
  CTGGTTTGCCTGCACTGGCTTCAGGAAGCTGGAGCCCTGTGACTCGAACGAGGAGAAGAGGCTGAAGCTG
  GCGAGAGCCATCTACCGAAAGTACATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCA
  CCAAGAGCTTCATAAAGGGCTGCATCATGAAGCAGCTGATCGATCCTGCCATGTTTGACCAGGCCCAGAC
  CGAAATCCAGGCCACTATGGAGGAAAACACCTATCCCTCCTTCCTTAAGTCTGATATTTATTTGGAATAT
  ACGAGGACAGGCTCGGAGAGCCCCAAAGTCTGTAGTGACCAGAGCTCTGGGTCAGGGACAGGGAAGGGCA
  TATCTGGATACCTGCCGACCTTAAATGAAGATGAGGAATGGAAGTGTGACCAGGACATGGATGAGGACGA
  TGGCAGAGACGCTGCTCCCCCCGGAAGACTCCCTCAGAAGCTGCTCCTGGAGACAGCTGCCCCGAGGGTC
  TCCTCCAGTAGACGGTACAGCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGGGAGCCAGTCAACCCCT
  ATTATGTCAATGCCGGCTATGCCCTGGCCCCAGCCACCAGTGCCAACGACAGCGAGCAGCAGAGCCTGTC
  CAGCGATGCAGACACCCTGTCCCTCACGGACAGCAGCGTGGATGGGATCCCCCCATACAGGATCCGTAAG
  CAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCGGGTGCCCCTACCTCACATTCCCCGCA
  CGTACCGGGTGCCGAAGGAGGTCCGCGTGGAGCCTCAGAAGTTCGCGGAGGAGCTCATCCACCGCCTGGA
  GGCTGTGCAGCGCACGCGGGAGGCCGAGGAGAAGCTGGAGGAGCGGCTGAAGCGCGTGCGCATGGAGGAG
  GAAGGTGAGGACGGCGATCCATCGTCAGGGCCCCCAGGGCCGTGTCACAAGCTGCCTCCCGCCCCCGCTT
  GGCACCACTTCCCGCCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGAGAACCC
  TGAGAGCATCCTGGACGAGCACGTACAGCGTGTGCTGAGGACACCTGGCCGCCAGTCGCCTGGGCCTGGC
  CATCGCTCCCCGGACAGTGGGCACGTGGCCAAGATGCCAGTGGCACTGGGGGGTGCCGCCTCGGGGCACG
  GGAAGCACGTACCCAAGTCAGGGGCGAAGCTGGACGCGGCCGGCCTGCACCACCACCGACACGTCCACCA
  CCACGTCCACCACAGCACAGCCCGGCCCAAGGAGCAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGC
  AGCTTCGCCTGGGGCCTGGAACCACACAGCCATGGGGCAAGGTCCCGAGGCTACTCAGAGAGTGTTGGCG
  CTGCCCCCAACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTGGCGTGCAAAAGAAATGCCAA
  GAAGGCTGAGTCGGGGAAGAGCGCCAGCACCGAGGTGCCAGGTGCCTCGGAGGATGCGGAGAAGAACCAG
  AAAATCATGCAGTGGATCATTGAGGGGGAAAAGGAGATCAGCAGGCACCGCAGGACCGGCCACGGGTCTT
  CGGGGACGAGGAAGCCACAGCCCCATGAGAACTCCAGACCCTTGTCCCTTGAGCACCCCTGGGCCGGCCC
  TCAGCTCCGGACCTCCGTGCAGCCCTCCCACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCT
  CCCAACCCCCTAACCCAGCTGGAGGAGGCGCGCCGACGTCTGGAGGAGGAAGAAAAGAGAGCCAGCCGAG
  CACCCTCCAAGCAGAGGACAAGATCGCAGAGGAAGGTGGGCGGCGGGAGTGCCCAGCCGTGTGACAGCAT
  CGTTGTGGCGTACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAGGGGCCGCGCTGTCACC
  CTGGGCCAGTTCAAGGAGCTGCTGACCAAAAAGGGCAGCTACAGATACTACTTCAAGAAAGTGAGCGACG
  AGTTTGACTGTGGGGTGGTGTTTGAGGAGGTTCGAGAGGACGAGGCCGTCCTGCCCGTCTTTGAGGAGAA
  GATCATCGGCAAAGTGGAGAAGGTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208349 representing NM_181050
  Red=Cloning site Green=Tags(s)
  
  MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGY
  EPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKL
  ARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEY
  TRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRV
  SSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRK
  QHRREMQESVQVNGRVPLPHIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEE
  EGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPG
  HRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQS
  SFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQ
  KIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPA
  PNPLTQLEEARRRLEEEEKRASRAPSKQRTRSQRKVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVT
  LGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181050
• ORF Size: 2478 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181050.1, NM_181050.2, NP_851393.1
• RefSeq Size: 3369 bp
• RefSeq ORF: 2481 bp
• Locus ID: 8312
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Pathways in cancer, Wnt signaling pathway
• MW: 91.5 kDa
• Gene Summary: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]