Axin 1 (AXIN1) (NM_181050) Human Untagged Clone

CAT#: SC319963

AXIN1 (untagged)-Human axin 1 (AXIN1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AXIN1
• Synonyms: AXIN; PPP1R49
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_181050.1 GCTGCCGCTGAGCGCATGGGCCGGGCCGCGCCGCGCCGCTCCGGGAGCCGGGCCCGGGGT
  CCCGCCACCACCGCGCGCGGGACAGATTGATTCACTTTGGAGCTGTAAGTACTGATGTAT
  TAGGGTGCAGCGCTCATTGTTCCTTGACGCAGAGTCCCAAAATGAATATCCAAGAGCAGG
  GTTTCCCCTTGGACCTCGGAGCAAGTTTCACCGAAGATGCTCCCCGACCCCCAGTGCCTG
  GTGAGGAGGGAGAACTGGTGTCCACAGACCCGAGGCCCGCCAGCTACAGTTTCTGCTCCG
  GGAAAGGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGATCTGG
  ACCTGGGGTATGAGCCTGAGGGCAGTGCCTCCCCCACCCCACCATACTTGAAGTGGGCTG
  AGTCACTGCATTCCCTGCTGGATGACCAAGATGGGATAAGCCTGTTCAGGACTTTCCTGA
  AGCAGGAGGGCTGTGCCGACTTGCTGGACTTCTGGTTTGCCTGCACTGGCTTCAGGAAGC
  TGGAGCCCTGTGACTCGAACGAGGAGAAGAGGCTGAAGCTGGCGAGAGCCATCTACCGAA
  AGTACATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCACCAAGAGCT
  TCATAAAGGGCTGCATCATGAAGCAGCTGATCGATCCTGCCATGTTTGACCAGGCCCAGA
  CCGAAATCCAGGCCACTATGGAGGAAAACACCTATCCCTCCTTCCTTAAGTCTGATATTT
  ATTTGGAATATACGAGGACAGGCTCGGAGAGCCCCAAAGTCTGTAGTGACCAGAGCTCTG
  GGTCAGGGACAGGGAAGGGCATATCTGGATACCTGCCGACCTTAAATGAAGATGAGGAAT
  GGAAGTGTGACCAGGACATGGATGAGGACGATGGCAGAGACGCTGCTCCCCCCGGAAGAC
  TCCCTCAGAAGCTGCTCCTGGAGACAGCTGCCCCGAGGGTCTCCTCCAGTAGACGGTACA
  GCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGGGAGCCAGTCAACCCCTATTATGTCA
  ATGCCGGCTATGCCCTGGCCCCAGCCACCAGTGCCAACGACAGCGAGCAGCAGAGCCTGT
  CCAGCGATGCAGACACCCTGTCCCTCACGGACAGCAGCGTGGATGGGATCCCCCCATACA
  GGATCCGTAAGCAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCGGGTGC
  CCCTACCTCACATTCCCCGCACGTACCGGGTGCCGAAGGAGGTCCGCGTGGAGCCTCAGA
  AGTTCGCGGAGGAGCTCATCCACCGCCTGGAGGCTGTGCAGCGCACGCGGGAGGCCGAGG
  AGAAGCTGGAGGAGCGGCTGAAGCGCGTGCGCATGGAGGAGGAAGGTGAGGACGGCGATC
  CATCGTCAGGGCCCCCAGGGCCGTGTCACAAGCTGCCTCCCGCCCCCGCTTGGCACCACT
  TCCCGCCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGAGAACC
  CTGAGAGCATCCTGGACGAGCACGTACAGCGTGTGCTGAGGACACCTGGCCGCCAGTCGC
  CTGGGCCTGGCCATCGCTCCCCGGACAGTGGGCACGTGGCCAAGATGCCAGTGGCACTGG
  GGGGTGCCGCCTCGGGGCACGGGAAGCACGTACCCAAGTCAGGGGCGAAGCTGGACGCGG
  CCGGCCTGCACCACCACCGACACGTCCACCACCACGTCCACCACAGCACAGCCCGGCCCA
  AGGAGCAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGCAGCTTCGCCTGGGGCCTGG
  AACCACACAGCCATGGGGCAAGGTCCCGAGGCTACTCAGAGAGTGTTGGCGCTGCCCCCA
  ACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTGGCGTGCAAAAGAAATGCCA
  AGAAGGCTGAGTCGGGGAAGAGCGCCAGCACCGAGGTGCCAGGTGCCTCGGAGGATGCGG
  AGAAGAACCAGAAAATCATGCAGTGGATCATTGAGGGGGAAAAGGAGATCAGCAGGCACC
  GCAGGACCGGCCACGGGTCTTCGGGGACGAGGAAGCCACAGCCCCATGAGAACTCCAGAC
  CCTTGTCCCTTGAGCACCCCTGGGCCGGCCCTCAGCTCCGGACCTCCGTGCAGCCCTCCC
  ACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCTCCCAACCCCCTAACCCAGC
  TGGAGGAGGCGCGCCGACGTCTGGAGGAGGAAGAAAAGAGAGCCAGCCGAGCACCCTCCA
  AGCAGAGGACAAGATCGCAGAGGAAGGTGGGCGGCGGGAGTGCCCAGCCGTGTGACAGCA
  TCGTTGTGGCGTACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAGGGGCC
  GCGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTGACCAAAAAGGGCAGCTACAGATACT
  ACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGGGTGGTGTTTGAGGAGGTTCGAGAGG
  ACGAGGCCGTCCTGCCCGTCTTTGAGGAGAAGATCATCGGCAAAGTGGAGAAGGTGGACT
  GATAGGCTGGTGGGCTGGCCGCTGTGCCAGGCGAGGCCCTTGGCGGGCACGGGTGTCACG
  GCCAGGCAGATGACCTCGTACTCAGGAGCCCGATGGGGAACAGTGTTGGGTGTACCACCC
  ATCCCTGTGGTCTACCCGTGTCTAGAGGCAGGTAGGGGGTCCCTCCAAGTGGTCCACAAG
  CTTCTGTCCTGCCCCCAAGGAGGCAGCCTGGACCACTCCTCATAGCAATACTTGGAGGGC
  CCAGCCCAAGTGAGGCAGCCGAGGTCCCTGCTGCCAGCTTCAGGTGACCCCCCCCCATCC
  CCCGGCACCTCCCTTGGGCACGTGTGCTGGGATCTACTTTCCCTCTGGGATTTGCCCACG
  TACCCAGGTCTGGCTGGGGCCCAGGCCCGGATGCAGAGGCCTGCAGGGCCTCTGTCAATT
  GTACGCGCCACCGAGTGCCTTCAACACAGCTTGTCTCTTGCCTGCCACTGTGTGAATCGG
  CGACGGAGCACTGCACCTGCCTCCAGCCGCCGGCTGTGCAGTCCTGGGTCCTCCTTTCTG
  AGGGCCCGTGTAAATATGTACATTTCTCAGGCTAGGCCAGCAGGGGCTGCCCGAGTCTGT
  TTTTCATGCGATGACACTTGTACAATTAATTATCTTTTCAAAGGTACTTGGATAATAATG
  AAATAAAACTGTTTTTGAACCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181050.1 unedited
  GGCGTCCTTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGGCACGAGGGCTGCCGCT
  GAGCGCATGGGCCGGGCCGCGCCGCGCCGCTCCGGGAGCCGGGCCCGGGGTCCCGCCACC
  ACCGCGCGCGGGACAGATTGATTCACTTTGGAGCTGTAAGTACTGATGTATTAGGGTGCA
  GCGCTCATTGTTCCTTGACGCAGAGTCCCAAAATGAATATCCAAGAGCAGGGTTTCCCCT
  TGGACCTCGGAGCAAGTTTCACCGAAGATGCTCCCCGACCCCCAGTGCCTGGTGAGGAGG
  GAGAACTGGTGTCCACAGACCCGAGGCCCGCCAGCTACAGTTTCTGCTCCGGGAAAGGTG
  TTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGATCTGGACCTGGGGT
  ATGAGCCTGAGGGCAGTGCCTCCCCCACCCCACCATACTTGAAGTGGGCTGAGTCACTGC
  ATTCCCTGCTGGATGACCAAGATGGGATAAGCCTGTTCAGGACTTTCCTGAAGCAGGAGG
  GCTGTGCCGACTTGCTGGACTTCTGGTTTGCCTGCACTGGCTTCAGGAAGCTGGAGCCCT
  GTGACTCGAACGAGGAGAAGAGGCTGAAGCTGGCGAGAGCCATCTACCGAAAGTACATTT
  CTTGATACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCACCAAGAGCTTCATAAAGG
  GCTGCATCATGAGCAGCTGATCGATCCTGCCATGTTTGACAGGCCCAGAACCGAAATCCA
  GGCACTTATGGAGGAAACACCTATCCTCTCCTAAGTTCTGATATTTATTTGGAATATCAG
  ACAGCTCGGAAGAGCCAAGTTCTATGAACCAGCTTTGTCAGGACAGGAAGCTATCTGTAC
  TGGCGACTATGAAAATGAGGATGAAGTTGTACAGAACATGGTGAAGAGCAATGCAAAACC
  TTCTC
• Restriction Sites: Please inquire
• ACCN: NM_181050
• ORF Size: 2481 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_181050.1, NP_851393.1
• RefSeq Size: 3369
• RefSeq ORF: 2481
• Locus ID: 8312
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Pathways in cancer, Wnt signaling pathway
• Gene Summary: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform b) is shorter, compared to isoform a. An upstream in-frame AUG is present that could extend the N-terminus of this isoform by 49 aa; however, there is no evidence that the upstream AUG is used, so the shorter N-terminus is being annotated.