Eph receptor B3 (EPHB3) (NM_004443) Human Tagged ORF Clone

CAT#: RC208364

EPHB3 (Myc-DDK-tagged)-Human EPH receptor B3 (EPHB3)

Product Images

Western blot validation of overexpression lysate (Cat# LY401410) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208364 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EPHB3 protein (Cat# TP308364). The protein was produced from HEK293T cells transfected with EPHB3 cDNA clone (Cat# RC208364) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EPHB3
• Synonyms: EK2; ETK2; HEK2; TYRO6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208364 representing NM_004443
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGAGCCCGCCCGCCGCCGCCGCCGTCGCCGCCGCCGGGGCTTCTGCCGCTGCTCCCTCCGCTGC
  TGCTGCTGCCGCTGCTGCTGCTGCCCGCCGGCTGCCGGGCGCTGGAAGAGACCCTCATGGACACAAAATG
  GGTAACATCTGAGTTGGCGTGGACATCTCATCCAGAAAGTGGGTGGGAAGAGGTGAGTGGCTACGATGAG
  GCCATGAATCCCATCCGCACATACCAGGTGTGTAATGTGCGCGAGTCAAGCCAGAACAACTGGCTTCGCA
  CGGGGTTCATCTGGCGGCGGGATGTGCAGCGGGTCTACGTGGAGCTCAAGTTCACTGTGCGTGACTGCAA
  CAGCATCCCCAACATCCCCGGCTCCTGCAAGGAGACCTTCAACCTCTTCTACTACGAGGCTGACAGCGAT
  GTGGCCTCAGCCTCCTCCCCCTTCTGGATGGAGAACCCCTACGTGAAAGTGGACACCATTGCACCCGATG
  AGAGCTTCTCGCGGCTGGATGCCGGCCGTGTCAACACCAAGGTGCGCAGCTTTGGGCCACTTTCCAAGGC
  TGGCTTCTACCTGGCCTTCCAGGACCAGGGCGCCTGCATGTCGCTCATCTCCGTGCGCGCCTTCTACAAG
  AAGTGTGCATCCACCACCGCAGGCTTCGCACTCTTCCCCGAGACCCTCACTGGGGCGGAGCCCACCTCGC
  TGGTCATTGCTCCTGGCACCTGCATCCCTAACGCCGTGGAGGTGTCGGTGCCACTCAAGCTCTACTGCAA
  CGGCGATGGGGAGTGGATGGTGCCTGTGGGTGCCTGCACCTGTGCCACCGGCCATGAGCCAGCTGCCAAG
  GAGTCCCAGTGCCGCCCCTGTCCCCCTGGGAGCTACAAGGCGAAGCAGGGAGAGGGGCCCTGCCTCCCAT
  GTCCCCCCAACAGCCGTACCACCTCCCCAGCCGCCAGCATCTGCACCTGCCACAATAACTTCTACCGTGC
  AGACTCGGACTCTGCGGACAGTGCCTGTACCACCGTGCCATCTCCACCCCGAGGTGTGATCTCCAATGTG
  AATGAAACCTCACTGATCCTCGAGTGGAGTGAGCCCCGGGACCTGGGTGGCCGGGATGACCTCCTGTACA
  ATGTCATCTGCAAGAAGTGCCATGGGGCTGGAGGGGCCTCAGCCTGCTCACGCTGTGATGACAACGTGGA
  GTTTGTGCCTCGGCAGCTGGGCCTGACGGAGCGCCGGGTCCACATCAGCCATCTGCTGGCCCACACGCGC
  TACACCTTTGAGGTGCAGGCGGTCAACGGTGTCTCGGGCAAGAGCCCTCTGCCGCCTCGTTATGCGGCCG
  TGAATATCACCACAAACCAGGCTGCCCCGTCTGAAGTGCCCACACTACGCCTGCACAGCAGCTCAGGCAG
  CAGCCTCACCCTATCCTGGGCACCCCCAGAGCGGCCCAACGGAGTCATCCTGGACTACGAGATGAAGTAC
  TTTGAGAAGAGCGAGGGCATCGCCTCCACAGTGACCAGCCAGATGAACTCCGTGCAGCTGGACGGGCTTC
  GGCCTGACGCCCGCTATGTGGTCCAGGTCCGTGCCCGCACAGTAGCTGGCTATGGGCAGTACAGCCGCCC
  TGCCGAGTTTGAGACCACAAGTGAGAGAGGCTCTGGGGCCCAGCAGCTCCAGGAGCAGCTTCCCCTCATC
  GTGGGCTCCGCTACAGCTGGGCTTGTCTTCGTGGTGGCTGTCGTGGTCATCGCTATCGTCTGCCTCAGGA
  AGCAGCGACACGGCTCTGATTCGGAGTACACGGAGAAGCTGCAGCAGTACATTGCTCCTGGAATGAAGGT
  TTATATTGACCCTTTTACCTACGAGGACCCTAATGAGGCTGTTCGGGAGTTTGCCAAGGAGATCGACGTG
  TCCTGCGTCAAGATCGAGGAGGTGATCGGAGCTGGGGAATTTGGGGAAGTGTGCCGTGGTCGACTGAAAC
  AGCCTGGCCGCCGAGAGGTGTTTGTGGCCATCAAGACGCTGAAGGTGGGCTACACCGAGAGGCAGCGGCG
  GGACTTCCTAAGCGAGGCCTCCATCATGGGTCAGTTTGATCACCCCAATATAATCCGGCTCGAGGGCGTG
  GTCACCAAAAGTCGGCCAGTTATGATCCTCACTGAGTTCATGGAAAACTGCGCCCTGGACTCCTTCCTCC
  GGCTCAACGATGGGCAGTTCACGGTCATCCAGCTGGTGGGCATGTTGCGGGGCATTGCTGCCGGCATGAA
  GTACCTGTCCGAGATGAACTATGTGCACCGCGACCTGGCTGCTCGCAACATCCTTGTCAACAGCAACCTG
  GTCTGCAAAGTCTCAGACTTTGGCCTCTCCCGCTTCCTGGAGGATGACCCCTCCGATCCTACCTACACCA
  GTTCCCTGGGCGGGAAGATCCCCATCCGCTGGACTGCCCCAGAGGCCATAGCCTATCGGAAGTTCACTTC
  TGCTAGTGATGTCTGGAGCTACGGAATTGTCATGTGGGAGGTCATGAGCTATGGAGAGCGACCCTACTGG
  GACATGAGCAACCAGGATGTCATCAATGCCGTGGAGCAGGATTACCGGCTGCCACCACCCATGGACTGTC
  CCACAGCACTGCACCAGCTCATGCTGGACTGCTGGGTGCGGGACCGGAACCTCAGGCCCAAATTCTCCCA
  GATTGTCAATACCCTGGACAAGCTCATCCGCAATGCTGCCAGCCTCAAGGTCATTGCCAGCGCTCAGTCT
  GGCATGTCACAGCCCCTCCTGGACCGCACGGTCCCAGATTACACAACCTTCACGACAGTTGGTGATTGGC
  TGGATGCCATCAAGATGGGGCGGTACAAGGAGAGCTTCGTCAGTGCGGGGTTTGCATCTTTTGACCTGGT
  GGCCCAGATGACGGCAGAAGACCTGCTCCGTATTGGGGTCACCCTGGCCGGCCACCAGAAGAAGATCCTG
  AGCAGTATCCAGGACATGCGGCTGCAGATGAACCAGACGCTGCCTGTGCAGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208364 representing NM_004443
  Red=Cloning site Green=Tags(s)
  
  MARARPPPPPSPPPGLLPLLPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDE
  AMNPIRTYQVCNVRESSQNNWLRTGFIWRRDVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSD
  VASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYK
  KCASTTAGFALFPETLTGAEPTSLVIAPGTCIPNAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAK
  ESQCRPCPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNV
  NETSLILEWSEPRDLGGRDDLLYNVICKKCHGAGGASACSRCDDNVEFVPRQLGLTERRVHISHLLAHTR
  YTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLRLHSSSGSSLTLSWAPPERPNGVILDYEMKY
  FEKSEGIASTVTSQMNSVQLDGLRPDARYVVQVRARTVAGYGQYSRPAEFETTSERGSGAQQLQEQLPLI
  VGSATAGLVFVVAVVVIAIVCLRKQRHGSDSEYTEKLQQYIAPGMKVYIDPFTYEDPNEAVREFAKEIDV
  SCVKIEEVIGAGEFGEVCRGRLKQPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGV
  VTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNL
  VCKVSDFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYW
  DMSNQDVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVIASAQS
  GMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVSAGFASFDLVAQMTAEDLLRIGVTLAGHQKKIL
  SSIQDMRLQMNQTLPVQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004443
• ORF Size: 2994 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004443.1, NM_004443.2, NM_004443.3, NP_004434.2
• RefSeq Size: 4234 bp
• RefSeq ORF: 2997 bp
• Locus ID: 2049
• Cytogenetics: 3q27.1
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• MW: 110.33 kDa
• Gene Summary: 'Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members. [provided by RefSeq, Mar 2010]'