PARVB (NM_013327) Human Tagged ORF Clone

CAT#: RC208490

PARVB (Myc-DDK-tagged)-Human parvin, beta (PARVB), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY415629) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208490 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PARVB protein (Cat# TP308490). The protein was produced from HEK293T cells transfected with PARVB cDNA clone (Cat# RC208490) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PARVB
• Synonyms: CGI-56
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208490 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCCGCGCCGCGCTCGCCCACCCCGCGGCCCCGCAGGATGAAGAAGGACGAGTCGTTCCTGGGCA
  AGCTGGGCGGCACCCTGGCCAGGAAGCGGAGGGCGCGCGAGGTGAGTGACCTGCAGGAAGAAGGCAAGAA
  TGCCATCAACTCACCGATGTCCCCCGCCCTGGCGGATGTTCACCCTGAAGACACCCAGCTCGAGGAGAAC
  GAGGAGCGCACGATGATTGACCCCACTTCCAAGGAAGACCCCAAGTTCAAGGAACTGGTCAAGGTCCTCC
  TCGACTGGATTAATGACGTGCTGGTGGAGGAGAGGATCATTGTGAAGCAGCTGGAGGAAGACCTGTATGA
  CGGCCAGGTGCTGCAGAAGCTCTTGGAAAAACTGGCAGGGTGCAAGCTGAATGTGGCTGAGGTGACACAG
  TCCGAAATAGGGCAGAAACAGAAGCTGCAGACGGTGCTGGAAGCAGTACATGACCTGCTGCGGCCCCGAG
  GCTGGGCGCTCCGGTGGAGCGTGGACTCAATTCACGGGAAGAACCTGGTGGCCATCCTCCACCTGCTGGT
  CTCTCTGGCCATGCACTTCAGGGCCCCCATCCGCCTTCCTGAGCATGTAACGGTGCAGGTGGTGGTCGTG
  CGGAAACGGGAAGGCCTGCTGCATTCCAGCCACATCTCGGAGGAGCTGACCACAACTACAGAGATGATGA
  TGGGCCGGTTCGAGCGGGATGCCTTCGACACGCTGTTCGACCACGCCCCGGATAAGCTCAGCGTGGTGAA
  GAAGTCTCTCATCACTTTTGTGAACAAGCACCTGAACAAGCTGAATTTGGAGGTGACGGAACTGGAGACC
  CAGTTTGCAGATGGCGTGTACCTGGTTCTGCTCATGGGCCTTCTGGAAGACTACTTTGTTCCTCTCCACC
  ACTTCTACCTGACTCCGGAAAGCTTCGATCAGAAGGTCCACAATGTGTCCTTTGCCTTTGAGCTGATGCT
  GGACGGAGGCCTCAAGAAACCCAAGGCTCGTCCTGAAGACGTGGTTAACTTGGACCTCAAATCCACCCTG
  AGGGTTCTTTACAACCTGTTCACCAAGTACAAGAACGTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208490 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSAPRSPTPRPRRMKKDESFLGKLGGTLARKRRAREVSDLQEEGKNAINSPMSPALADVHPEDTQLEEN
  EERTMIDPTSKEDPKFKELVKVLLDWINDVLVEERIIVKQLEEDLYDGQVLQKLLEKLAGCKLNVAEVTQ
  SEIGQKQKLQTVLEAVHDLLRPRGWALRWSVDSIHGKNLVAILHLLVSLAMHFRAPIRLPEHVTVQVVVV
  RKREGLLHSSHISEELTTTTEMMMGRFERDAFDTLFDHAPDKLSVVKKSLITFVNKHLNKLNLEVTELET
  QFADGVYLVLLMGLLEDYFVPLHHFYLTPESFDQKVHNVSFAFELMLDGGLKKPKARPEDVVNLDLKSTL
  RVLYNLFTKYKNVE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013327
• ORF Size: 1092 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013327.2, NM_013327.3, NM_013327.4, NP_037459.2
• RefSeq Size: 1725 bp
• RefSeq ORF: 1095 bp
• Locus ID: 29780
• Domains: CH
• Protein Pathways: Focal adhesion
• MW: 41.7 kDa
• Gene Summary: This gene encodes a member of the parvin family of actin-binding proteins, which play a role in cytoskeleton organization and cell adhesion. These proteins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. This family member binds to alphaPIX and alpha-actinin, and it can inhibit the activity of integrin-linked kinase. This protein also functions in tumor suppression. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]