GABA B Receptor 1 (GABBR1) (NM_021903) Human Tagged ORF Clone

CAT#: RC208503L3

Lenti-ORF clone of GABBR1 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) B receptor, 1 (GABBR1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GABBR1
• Synonyms: GABABR1; GABBR1-3; GB1; GPRC3A
• Vector: pLenti-C-Myc-DDK-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC208503).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_021903
• ORF Size: 2532 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021903.1, NP_068703.1
• RefSeq Size: 4039 bp
• RefSeq ORF: 2535 bp
• Locus ID: 2550
• Cytogenetics: 6p22.1
• Domains: 7tm_3, ANF_receptor
• Protein Families: Druggable Genome, GPCR, Secreted Protein, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 92.2 kDa
• Gene Summary: 'This gene encodes a receptor for gamma-aminobutyric acid (GABA), which is the main inhibitory neurotransmitter in the mammalian central nervous system. This receptor functions as a heterodimer with GABA(B) receptor 2. Defects in this gene may underlie brain disorders such as schizophrenia and epilepsy. Alternative splicing generates multiple transcript variants, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jan 2016]'