GABA B Receptor 1 (GABBR1) (NM_021903) Human Tagged ORF Clone

CAT#: RG208503

GABBR1 (GFP-tagged) - Human gamma-aminobutyric acid (GABA) B receptor, 1 (GABBR1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GABBR1
• Synonyms: GABABR1; GABBR1-3; GB1; GPRC3A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208503 representing NM_021903
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCGGGGCCCCTTTTGCCCGGGTGGGGTGGCCACTGCCGCTTCTGGTTGTGATGGCGGCAGGGG
  TGGCTCCGGTGTGGGCCTCCCACTCCCCCCATCTCCCGCGGCCTCACTCGCGGGTCCCCCCGCACCCCTC
  CTCAGAACGGCGCGCAGTGTACATCGGGGCACTGTTTCCCATGAGCGGGGGCTGGCCAGGGGGCCAGGCC
  TGCCAGCCCGCGGTGGAGATGGCGCTGGAGGACGTGAATAGCCGCAGGGACATCCTGCCGGACTATGAGC
  TCAAGCTCATCCACCACGACAGCAAGTGTGATCCAGGCCAAGCCACCAAGTACCTATATGAGCTGCTCTA
  CAACGACCCTATCAAGATCATCCTTATGCCTGGCTGCAGCTCTGTCTCCACGCTGGTGGCTGAGGCTGCT
  AGGATGTGGAACCTCATTGTGCTTTCCTATGGCTCCAGCTCACCAGCCCTGTCAAACCGGCAGCGTTTCC
  CCACTTTCTTCCGAACGCACCCATCAGCCACACTCCACAACCCTACCCGCGTGAAACTCTTTGAAAAGTG
  GGGCTGGAAGAAGATTGCTACCATCCAGCAGACCACTGAGGTCTTCACTTCGACTCTGGACGACCTGGAG
  GAACGAGTGAAGGAGGCTGGAATTGAGATTACTTTCCGCCAGAGTTTCTTCTCAGATCCAGCTGTGCCCG
  TCAAAAACCTGAAGCGCCAGGATGCCCGAATCATCGTGGGACTTTTCTATGAGACTGAAGCCCGGAAAGT
  TTTTTGTGAGGTGTACAAGGAGCGTCTCTTTGGGAAGAAGTACGTCTGGTTCCTCATTGGGTGGTATGCT
  GACAATTGGTTCAAGATCTACGACCCTTCTATCAACTGCACAGTGGATGAGATGACTGAGGCGGTGGAGG
  GCCACATCACAACTGAGATTGTCATGCTGAATCCTGCCAATACCCGCAGCATTTCCAACATGACATCCCA
  GGAATTTGTGGAGAAACTAACCAAGCGACTGAAAAGACACCCTGAGGAGACAGGAGGCTTCCAGGAGGCA
  CCGCTGGCCTATGATGCCATCTGGGCCTTGGCACTGGCCCTGAACAAGACATCTGGAGGAGGCGGCCGTT
  CCGGCGTGCGCCTGGAGGACTTCAACTACAACAACCAGACCATTACCGACCAAATCTACCGGGCAATGAA
  CTCCTCGTCCTTTGAGGGTGTCTCTGGCCATGTGGTGTTTGATGCCAGCGGCTCTCGGATGGCATGGACG
  CTTATCGAGCAGCTTCAGGGTGGCAGCTACAAGAAGATTGGCTACTATGACAGCACCAAGGATGATCTTT
  CCTGGTCCAAAACAGATAAATGGATTGGAGGGTCCCCCCCAGCTGACCAGACCCTGGTCATCAAGACATT
  CCGCTTCCTGTCACAGAAACTCTTTATCTCCGTCTCAGTTCTCTCCAGCCTGGGCATTGTCCTAGCTGTT
  GTCTGTCTGTCCTTTAACATCTACAACTCACATGTCCGTTATATCCAGAACTCACAGCCCAACCTGAACA
  ACCTGACTGCTGTGGGCTGCTCACTGGCTTTAGCTGCTGTCTTCCCCCTGGGGCTCGATGGTTACCACAT
  TGGGAGGAACCAGTTCCCTTTCGTCTGCCAGGCCCGCCTCTGGCTCCTGGGCCTGGGCTTTAGTCTGGGC
  TACGGTTCCATGTTCACCAAGATTTGGTGGGTCCACACGGTCTTCACAAAGAAGGAAGAAAAGAAGGAGT
  GGAGGAAGACTCTGGAACCCTGGAAGCTGTATGCCACAGTGGGCCTGCTGGTGGGCATGGATGTCCTCAC
  TCTCGCCATCTGGCAGATCGTGGACCCTCTGCACCGGACCATTGAGACATTTGCCAAGGAGGAACCTAAG
  GAAGATATTGACGTCTCTATTCTGCCCCAGCTGGAGCATTGCAGCTCCAGGAAGATGAATACATGGCTTG
  GCATTTTCTATGGTTACAAGGGGCTGCTGCTGCTGCTGGGAATCTTCCTTGCTTATGAGACCAAGAGTGT
  GTCCACTGAGAAGATCAATGATCACCGGGCTGTGGGCATGGCTATCTACAATGTGGCAGTCCTGTGCCTC
  ATCACTGCTCCTGTCACCATGATTCTGTCCAGCCAGCAGGATGCAGCCTTTGCCTTTGCCTCTCTTGCCA
  TAGTTTTCTCCTCCTATATCACTCTTGTTGTGCTCTTTGTGCCCAAGATGCGCAGGCTGATCACCCGAGG
  GGAATGGCAGTCGGAGGCGCAGGACACCATGAAGACAGGGTCATCGACCAACAACAACGAGGAGGAGAAG
  TCCCGGCTGTTGGAGAAGGAGAACCGTGAACTGGAAAAGATCATTGCTGAGAAAGAGGAGCGTGTCTCTG
  AACTGCGCCATCAACTCCAGTCTCGGCAGCAGCTCCGCTCCCGGCGCCACCCACCGACACCCCCAGAACC
  CTCTGGGGGCCTGCCCAGGGGACCCCCTGAGCCCCCCGACCGGCTTAGCTGTGATGGGAGTCGAGTGCAT
  TTGCTTTATAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208503 representing NM_021903
  Red=Cloning site Green=Tags(s)
  
  MGPGAPFARVGWPLPLLVVMAAGVAPVWASHSPHLPRPHSRVPPHPSSERRAVYIGALFPMSGGWPGGQA
  CQPAVEMALEDVNSRRDILPDYELKLIHHDSKCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAA
  RMWNLIVLSYGSSSPALSNRQRFPTFFRTHPSATLHNPTRVKLFEKWGWKKIATIQQTTEVFTSTLDDLE
  ERVKEAGIEITFRQSFFSDPAVPVKNLKRQDARIIVGLFYETEARKVFCEVYKERLFGKKYVWFLIGWYA
  DNWFKIYDPSINCTVDEMTEAVEGHITTEIVMLNPANTRSISNMTSQEFVEKLTKRLKRHPEETGGFQEA
  PLAYDAIWALALALNKTSGGGGRSGVRLEDFNYNNQTITDQIYRAMNSSSFEGVSGHVVFDASGSRMAWT
  LIEQLQGGSYKKIGYYDSTKDDLSWSKTDKWIGGSPPADQTLVIKTFRFLSQKLFISVSVLSSLGIVLAV
  VCLSFNIYNSHVRYIQNSQPNLNNLTAVGCSLALAAVFPLGLDGYHIGRNQFPFVCQARLWLLGLGFSLG
  YGSMFTKIWWVHTVFTKKEEKKEWRKTLEPWKLYATVGLLVGMDVLTLAIWQIVDPLHRTIETFAKEEPK
  EDIDVSILPQLEHCSSRKMNTWLGIFYGYKGLLLLLGIFLAYETKSVSTEKINDHRAVGMAIYNVAVLCL
  ITAPVTMILSSQQDAAFAFASLAIVFSSYITLVVLFVPKMRRLITRGEWQSEAQDTMKTGSSTNNNEEEK
  SRLLEKENRELEKIIAEKEERVSELRHQLQSRQQLRSRRHPPTPPEPSGGLPRGPPEPPDRLSCDGSRVH
  LLYK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021903
• ORF Size: 2532 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021903.1, NP_068703.1
• RefSeq Size: 4039 bp
• RefSeq ORF: 2535 bp
• Locus ID: 2550
• Cytogenetics: 6p22.1
• Domains: 7tm_3, ANF_receptor
• Protein Families: Druggable Genome, GPCR, Secreted Protein, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes a receptor for gamma-aminobutyric acid (GABA), which is the main inhibitory neurotransmitter in the mammalian central nervous system. This receptor functions as a heterodimer with GABA(B) receptor 2. Defects in this gene may underlie brain disorders such as schizophrenia and epilepsy. Alternative splicing generates multiple transcript variants, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jan 2016]'