SMARCC1 (NM_003074) Human Tagged ORF Clone

CAT#: RC208534

SMARCC1 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 (SMARCC1)

Product Images

Coomassie blue staining of purified SMARCC1 protein (Cat# TP308534). The protein was produced from HEK293T cells transfected with SMARCC1 cDNA clone (Cat# RC208534) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMARCC1
• Synonyms: BAF155; CRACC1; Rsc8; SRG3; SWI3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208534 representing NM_003074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCAGCGGCGGGCGGCGGCGGGCCGGGGACAGCGGTAGGCGCCACGGGCTCGGGGATTGCGGCGG
  CAGCCGCAGGCCTAGCTGTTTATCGACGGAAGGATGGGGGCCCGGCCACCAAGTTTTGGGAGAGCCCGGA
  GACGGTGTCCCAGCTGGATTCGGTGCGGGTCTGGCTGGGCAAGCACTACAAGAAGTATGTTCATGCGGAT
  GCTCCTACCAATAAAACACTGGCTGGGCTGGTGGTGCAGCTTCTTCAGTTCCAGGAAGATGCCTTTGGGA
  AGCATGTCACCAACCCGGCCTTCACCAAACTCCCTGCAAAGTGTTTCATGGATTTCAAAGCTGGAGGCGC
  CTTATGTCACATTCTTGGGGCTGCTTACAAGTATAAAAATGAACAGGGATGGCGGAGGTTTGACCTACAG
  AACCCATCTCGAATGGATCGTAATGTGGAAATGTTTATGAACATTGAAAAAACATTGGTGCAGAACAATT
  GTTTGACCAGACCCAACATCTACCTCATTCCAGACATTGATCTGAAGTTGGCTAACAAATTGAAAGATAT
  CATCAAACGACATCAGGGAACATTTACGGATGAGAAGTCAAAAGCTTCCCACCACATTTACCCATATTCT
  TCCTCACAAGACGATGAAGAATGGTTGAGACCGGTGATGAGAAAAGAGAAGCAAGTGTTAGTGCATTGGG
  GCTTTTACCCAGACAGCTATGATACTTGGGTCCATAGTAATGATGTTGATGCTGAAATTGAAGATCCACC
  AATTCCAGAAAAACCATGGAAGGTTCATGTGAAATGGATTTTGGACACTGATATTTTCAATGAATGGATG
  AATGAGGAGGATTATGAGGTGGATGAAAATAGGAAGCCTGTGAGTTTTCGTCAGCGGATTTCAACCAAGA
  ATGAAGAGCCAGTCAGAAGTCCAGAAAGAAGAGATAGAAAAGCATCAGCTAATGCTCGAAAGAGGAAACA
  TTCGCCTTCGCCTCCCCCTCCGACACCAACAGAATCACGGAAGAAGAGTGGGAAGAAAGGCCAAGCTAGC
  CTTTATGGGAAGCGCAGAAGTCAGAAAGAGGAAGATGAGCAAGAAGATCTAACCAAGGATATGGAAGACC
  CAACACCTGTACCCAATATAGAAGAAGTAGTACTTCCCAAAAATGTGAACCTAAAGAAAGATAGTGAAAA
  TACACCTGTTAAAGGAGGAACTGTAGCGGATCTAGATGAGCAGGATGAAGAAACAGTCACAGCAGGAGGA
  AAGGAAGATGAAGATCCTGCCAAAGGTGATCAGAGTCGATCAGTTGACCTTGGGGAAGATAATGTGACAG
  AGCAGACCAATCACATTATTATTCCTAGTTATGCATCATGGTTTGATTATAACTGTATTCATGTGATTGA
  ACGGCGTGCTCTTCCTGAGTTCTTCAATGGAAAAAACAAATCCAAGACTCCAGAAATATACTTGGCATAT
  CGAAATTTTATGATTGACACGTATCGTCTAAACCCCCAAGAGTATTTAACTAGCACTGCTTGTCGGAGGA
  ACTTGACTGGAGATGTGTGTGCTGTGATGAGGGTCCATGCCTTTTTAGAGCAGTGGGGACTCGTTAATTA
  CCAAGTTGACCCGGAAAGTAGACCCATGGCAATGGGACCTCCTCCTACTCCTCATTTTAATGTATTAGCT
  GATACCCCCTCTGGGCTTGTGCCTCTGCATCTTCGATCACCTCAGGTTCCTGCTGCTCAACAGATGCTAA
  ATTTTCCTGAGAAAAACAAGGAAAAACCAGTTGATTTGCAGAACTTTGGTCTCCGTACTGACATTTACTC
  CAAGAAAACATTAGCAAAGAGTAAAGGTGCTAGTGCTGGAAGAGAATGGACTGAACAGGAGACCCTTCTA
  CTCCTGGAGGCCCTGGAGATGTACAAGGATGATTGGAACAAAGTGTCGGAACATGTTGGAAGTCGTACTC
  AGGATGAATGCATCCTCCACTTTTTGAGACTTCCCATTGAGGACCCATACCTTGAGAATTCAGATGCTTC
  CCTTGGGCCTTTGGCCTACCAGCCTGTCCCCTTCAGTCAGTCAGGAAATCCAGTTATGAGTACTGTTGCT
  TTTTTGGCATCTGTGGTGGACCCTCGCGTGGCATCTGCTGCAGCAAAAGCGGCTTTGGAGGAGTTTTCTC
  GGGTCCGGGAGGAGGTACCACTGGAATTGGTTGAAGCTCATGTCAAGAAAGTACAAGAAGCAGCACGAGC
  CTCTGGGAAAGTGGATCCCACCTACGGTCTGGAGAGCAGCTGCATTGCAGGCACAGGGCCCGATGAGCCA
  GAGAAGCTTGAAGGAGCTGAAGAGGAAAAAATGGAAGCCGACCCTGATGGTCAGCAGCCTGAAAAGGCAG
  AAAATAAAGTGGAAAATGAAACGGATGAAGGTGATAAAGCACAAGATGGAGAAAATGAAAAAAATAGTGA
  AAAGGAACAGGATAGTGAAGTGAGTGAGGATACCAAATCAGAAGAAAAGGAGACTGAAGAGAACAAAGAA
  CTCACTGATACATGTAAAGAAAGAGAAAGTGATACTGGGAAGAAGAAAGTAGAACATGAAATTTCCGAAG
  GAAATGTTGCCACAGCCGCAGCAGCTGCTCTTGCCTCAGCGGCTACCAAAGCCAAGCACCTGGCTGCAGT
  GGAAGAAAGAAAGATCAAGTCCCTGGTAGCTCTCTTGGTTGAGACACAAATGAAGAAACTAGAGATCAAA
  CTTCGACATTTTGAAGAGCTGGAAACTATCATGGACAGAGAGAAAGAAGCTCTAGAACAACAGAGGCAGC
  AGTTGCTTACTGAACGCCAAAACTTCCACATGGAACAGCTGAAGTATGCTGAATTACGAGCACGACAGCA
  AATGGAACAGCAGCAGCATGGCCAGAACCCTCAACAGGCACACCAGCACTCAGGAGGACCTGGCCTGGCC
  CCACTTGGAGCAGCAGGGCACCCTGGCATGATGCCTCATCAACAGCCCCCTCCCTACCCTCTGATGCACC
  ACCAGATGCCACCACCTCATCCACCCCAGCCAGGTCAGATACCAGGCCCAGGTTCCATGATGCCCGGGCA
  GCACATGCCAGGCCGCATGATTCCCACTGTTGCAGCCAACATCCACCCCTCTGGGAGTGGCCCTACCCCT
  CCTGGCATGCCACCAATGCCAGGAAACATCTTAGGACCCCGGGTACCCCTGACAGCACCTAACGGCATGT
  ATCCCCCTCCACCACAGCAGCAGCCACCGCCACCACCACCTGCAGATGGGGTCCCTCCGCCTCCTGCTCC
  TGGCCCGCCAGCCTCAGCTGCTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208534 representing NM_003074
  Red=Cloning site Green=Tags(s)
  
  MAAAAGGGGPGTAVGATGSGIAAAAAGLAVYRRKDGGPATKFWESPETVSQLDSVRVWLGKHYKKYVHAD
  APTNKTLAGLVVQLLQFQEDAFGKHVTNPAFTKLPAKCFMDFKAGGALCHILGAAYKYKNEQGWRRFDLQ
  NPSRMDRNVEMFMNIEKTLVQNNCLTRPNIYLIPDIDLKLANKLKDIIKRHQGTFTDEKSKASHHIYPYS
  SSQDDEEWLRPVMRKEKQVLVHWGFYPDSYDTWVHSNDVDAEIEDPPIPEKPWKVHVKWILDTDIFNEWM
  NEEDYEVDENRKPVSFRQRISTKNEEPVRSPERRDRKASANARKRKHSPSPPPPTPTESRKKSGKKGQAS
  LYGKRRSQKEEDEQEDLTKDMEDPTPVPNIEEVVLPKNVNLKKDSENTPVKGGTVADLDEQDEETVTAGG
  KEDEDPAKGDQSRSVDLGEDNVTEQTNHIIIPSYASWFDYNCIHVIERRALPEFFNGKNKSKTPEIYLAY
  RNFMIDTYRLNPQEYLTSTACRRNLTGDVCAVMRVHAFLEQWGLVNYQVDPESRPMAMGPPPTPHFNVLA
  DTPSGLVPLHLRSPQVPAAQQMLNFPEKNKEKPVDLQNFGLRTDIYSKKTLAKSKGASAGREWTEQETLL
  LLEALEMYKDDWNKVSEHVGSRTQDECILHFLRLPIEDPYLENSDASLGPLAYQPVPFSQSGNPVMSTVA
  FLASVVDPRVASAAAKAALEEFSRVREEVPLELVEAHVKKVQEAARASGKVDPTYGLESSCIAGTGPDEP
  EKLEGAEEEKMEADPDGQQPEKAENKVENETDEGDKAQDGENEKNSEKEQDSEVSEDTKSEEKETEENKE
  LTDTCKERESDTGKKKVEHEISEGNVATAAAAALASAATKAKHLAAVEERKIKSLVALLVETQMKKLEIK
  LRHFEELETIMDREKEALEQQRQQLLTERQNFHMEQLKYAELRARQQMEQQQHGQNPQQAHQHSGGPGLA
  PLGAAGHPGMMPHQQPPPYPLMHHQMPPPHPPQPGQIPGPGSMMPGQHMPGRMIPTVAANIHPSGSGPTP
  PGMPPMPGNILGPRVPLTAPNGMYPPPPQQQPPPPPPADGVPPPPAPGPPASAAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003074
• ORF Size: 3315 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003074.2, NM_003074.3, NP_003065.2, NP_003065.3
• RefSeq Size: 5780
• RefSeq ORF: 3318
• Locus ID: 6599
• Domains: CHROMO, myb_DNA-binding, SWIRM
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• MW: 122.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq, Jul 2008]