PRDM14 (NM_024504) Human Tagged ORF Clone

CAT#: RC208569

PRDM14 (Myc-DDK-tagged)-Human PR domain containing 14 (PRDM14)

Product Images

Western blot validation of overexpression lysate (Cat# LY402995) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208569 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRDM14
• Synonyms: PFM11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208569 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTACCCCGGCCAAGTGAGGCCGTGCCTCAGGACAAGGTGTGCTACCCGCCGGAGAGCAGCCCGC
  AGAACCTGGCCGCGTACTACACGCCTTTCCCGTCCTATGGACACTACAGAAACAGCCTGGCCACCGTGGA
  GGAAGACTTCCAACCTTTCCGGCAGCTGGAGGCCGCAGCGTCTGCTGCCCCCGCCATGCCCCCCTTCCCC
  TTCCGGATGGCGCCTCCCTTGCTGAGCCCGGGTCTGGGCCTACAGAGGGAGCCTCTCTACGATCTGCCCT
  GGTACAGCAAGCTGCCACCGTGGTACCCAATTCCCCACGTCCCCAGGGAAGTGCCGCCCTTCCTGAGCAG
  CAGCCACGAGTACGCGGGTGCCAGCAGTGAAGATCTGGGCCACCAAATCATTGGTGGCGACAACGAGAGT
  GGCCCGTGTTGTGGACCTGACACTTTAATTCCACCGCCCCCTGCGGATGCTTCTCTGTTACCTGAGGGGC
  TGAGGACCTCCCAGTTATTACCTTGCTCACCCAGCAAGCAGTCAGAGGATGGTCCCAAACCCTCCAACCA
  AGAAGGGAAGTCCCCTGCTCGGTTCCAGTTCACGGAGGAGGACCTGCACTTCGTTCTGTACGGGGTCACT
  CCCAGCCTGGAGCACCCAGCCAGCCTGCACCATGCGATTTCAGGCCTCCTGGTCCCCCCAGACAGCTCTG
  GATCTGATTCTCTTCCTCAAACTCTGGATGAAGACTCCCTTCAACTTCCAGAAGGTCTATGCCTCATGCA
  GACGGTGTTTGGTGAAGTCCCACATTTTGGTGTGTTCTGCAGTAGTTTTATCGCCAAAGGAGTCAGGTTT
  GGGCCCTTTCAAGGTAAAGTGGTCAATGCCAGTGAAGTGAAGACCTACGGAGACAATTCTGTGATGTGGG
  AGATCTTTGAAGATGGTCATTTGAGCCACTTTATAGATGGAAAAGGAGGTACGGGGAACTGGATGTCCTA
  TGTCAACTGTGCCCGCTTCCCCAAGGAGCAGAACCTAGTTGCTGTGCAGTGTCAAGGGCATATATTTTAT
  GAGAGCTGCAAAGAGATTCATCAGAACCAAGAGCTCCTTGTGTGGTATGGAGACTGCTATGAGAAATTTC
  TGGATATTCCTGTGAGCCTTCAGGTCACAGAGCCGGGGAAGCAGCCATCTGGGCCCTCTGAAGAGTCTGC
  AGAAGGCTACAGATGTGAAAGATGTGGGAAGGTATTTACCTACAAATATTACAGAGATAAGCACCTCAAG
  TACACCCCCTGTGTGGACAAGGGCGATAGGAAATTTCCCTGTTCTCTCTGCAAACGATCCTTTGAGAAGC
  GGGACCGGCTTCGGATCCACATTCTTCATGTTCATGAGAAGCACCGGCCTCACAAGTGTTCTACATGTGG
  GAAATGTTTCTCTCAGTCTTCCAGCCTAAACAAACACATGCGAGTCCACTCTGGAGACAGACCATACCAG
  TGTGTGTATTGTACTAAGAGGTTCACAGCCTCCAGCATACTCCGCACACACATCAGGCAGCACTCCGGGG
  AGAAGCCCTTCAAATGCAAGTACTGTGGTAAATCTTTTGCATCCCATGCTGCCCATGACAGCCATGTCCG
  GCGTTCACACAAGGAGGACGATGGCTGCTCATGCAGCATCTGTGGGAAAATCTTCTCAGATCAAGAAACA
  TTCTACTCCCACATGAAGTTTCATGAAGACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208569 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALPRPSEAVPQDKVCYPPESSPQNLAAYYTPFPSYGHYRNSLATVEEDFQPFRQLEAAASAAPAMPPFP
  FRMAPPLLSPGLGLQREPLYDLPWYSKLPPWYPIPHVPREVPPFLSSSHEYAGASSEDLGHQIIGGDNES
  GPCCGPDTLIPPPPADASLLPEGLRTSQLLPCSPSKQSEDGPKPSNQEGKSPARFQFTEEDLHFVLYGVT
  PSLEHPASLHHAISGLLVPPDSSGSDSLPQTLDEDSLQLPEGLCLMQTVFGEVPHFGVFCSSFIAKGVRF
  GPFQGKVVNASEVKTYGDNSVMWEIFEDGHLSHFIDGKGGTGNWMSYVNCARFPKEQNLVAVQCQGHIFY
  ESCKEIHQNQELLVWYGDCYEKFLDIPVSLQVTEPGKQPSGPSEESAEGYRCERCGKVFTYKYYRDKHLK
  YTPCVDKGDRKFPCSLCKRSFEKRDRLRIHILHVHEKHRPHKCSTCGKCFSQSSSLNKHMRVHSGDRPYQ
  CVYCTKRFTASSILRTHIRQHSGEKPFKCKYCGKSFASHAAHDSHVRRSHKEDDGCSCSICGKIFSDQET
  FYSHMKFHEDY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024504
• ORF Size: 1713 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024504.1, NM_024504.2, NM_024504.3, NP_078780.1
• RefSeq Size: 2344 bp
• RefSeq ORF: 1716 bp
• Locus ID: 63978
• Protein Families: Transcription Factors
• MW: 64.1 kDa
• Gene Summary: This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011]