CITED4 (NM_133467) Human Tagged ORF Clone

CAT#: RC208620

CITED4 (Myc-DDK-tagged)-Human Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 4 (CITED4)

Product Images

Western blot validation of overexpression lysate (Cat# LY408804) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208620 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CITED4
• Synonyms: Asp-rich carboxy-terminal domain, 4; Cbp; OTTHUMP00000009206; p300-interacting transactivator, with Glu; transcriptional co-activator 4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208620 representing NM_133467
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACCACCTGATGCTCGCCGAGGGCTACCGCCTGGTGCAGAGGCCGCCGTCCGCCGCGGCCGCCC
  ATGGCCCTCATGCGCTCCGGACTCTGCCGCCGTACGCGGGCCCGGGCCTGGACAGTGGGCTGAGGCCGCG
  GGGGGCTCCGCTGGGGCCGCCGCCGCCCCGCCAACCCGGGGCCCTGGCGTACGGGGCCTTCGGGCCGCCG
  TCCTCCTTCCAGCCCTTTCCGGCCGTGCCTCCGCCGGCCGCGGGCATCGCGCACCTGCAGCCTGTGGCGA
  CGCCGTACCCCGGCCGCGCGGCCGCGCCCCCCAACGCTCCGGGAGGCCCCCCGGGCCCGCAGCCGGCGCC
  AAGCGCCGCAGCCCCGCCGCCGCCCGCGCACGCCCTGGGCGGCATGGACGCCGAACTCATCGACGAGGAG
  GCGCTGACGTCGCTGGAGCTGGAGCTCGGGCTGCACCGCGTGCGCGAGCTGCCCGAGCTCTTCCTGGGCC
  AGAGCGAGTTCGACTGCTTCTCGGACTTGGGGTCCGCGCCGCCCGCCGGCTCCGTGAGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208620 representing NM_133467
  Red=Cloning site Green=Tags(s)
  
  MADHLMLAEGYRLVQRPPSAAAAHGPHALRTLPPYAGPGLDSGLRPRGAPLGPPPPRQPGALAYGAFGPP
  SSFQPFPAVPPPAAGIAHLQPVATPYPGRAAAPPNAPGGPPGPQPAPSAAAPPPPAHALGGMDAELIDEE
  ALTSLELELGLHRVRELPELFLGQSEFDCFSDLGSAPPAGSVSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133467
• ORF Size: 552 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133467.1, NM_133467.2, NP_597724.1
• RefSeq Size: 1312 bp
• RefSeq ORF: 555 bp
• Locus ID: 163732
• Cytogenetics: 1p35-p34
• Protein Families: Transcription Factors
• MW: 18.4 kDa
• Gene Summary: The protein encoded by this intronless gene belongs to the CITED family of transcriptional coactivators that bind to several proteins, including CREB-binding protein (CBP) and p300, via a conserved 32 aa C-terminal motif, and regulate gene transcription. This protein also interacts with transcription factor AP2 (TFAP2), and thus may function as a co-activator for TFAP2. Hypermethylation and transcriptional downregulation of this gene has been observed in oligodendroglial tumors with deletions of chromosomal arms 1p and 19q, and associated with longer recurrence-free and overall survival of patients with oligodendroglial tumors. [provided by RefSeq, Aug 2011]