Claudin 9 (CLDN9) (NM_020982) Human Tagged ORF Clone

CAT#: RC208701

CLDN9 (Myc-DDK-tagged)-Human claudin 9 (CLDN9)

Product Images

Western blot validation of overexpression lysate (Cat# LY412160) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208701 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CLDN9 protein (Cat# TP308701). The protein was produced from HEK293T cells transfected with CLDN9 cDNA clone (Cat# RC208701) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLDN9
• Synonyms: claudin 9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208701 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCGACCGGCTTAGAACTGCTGGGCATGACCCTGGCTGTGCTGGGCTGGCTGGGGACCCTGGTGT
  CCTGCGCCCTGCCCCTGTGGAAGGTGACCGCCTTCATCGGCAACAGCATCGTGGTGGCCCAGGTGGTGTG
  GGAGGGCCTGTGGATGTCCTGCGTGGTGCAGAGCACGGGCCAGATGCAGTGCAAGGTGTACGACTCACTG
  CTGGCTCTGCCGCAGGACCTGCAGGCCGCACGTGCCCTCTGTGTCATTGCCCTCCTGCTGGCCCTGCTTG
  GCCTCCTGGTGGCCATCACAGGTGCCCAGTGTACCACGTGTGTGGAGGACGAAGGTGCCAAGGCCCGTAT
  CGTGCTCACCGCGGGGGTCATCCTCCTCCTCGCCGGCATCCTGGTGCTCATCCCTGTGTGCTGGACGGCG
  CACGCCATCATCCAGGACTTCTACAACCCCCTGGTGGCTGAGGCCCTCAAGCGGGAGCTGGGGGCCTCCC
  TCTACCTGGGCTGGGCGGCGGCTGCACTGCTTATGCTGGGCGGGGGGCTCCTCTGCTGCACGTGCCCCCC
  GCCCCAGGTCGAGCGGCCCCGCGGACCTCGGCTGGGCTACTCCATCCCCTCCCGCTCGGGTGCATCTGGA
  CTGGACAAGAGGGACTACGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208701 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASTGLELLGMTLAVLGWLGTLVSCALPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSL
  LALPQDLQAARALCVIALLLALLGLLVAITGAQCTTCVEDEGAKARIVLTAGVILLLAGILVLIPVCWTA
  HAIIQDFYNPLVAEALKRELGASLYLGWAAAALLMLGGGLLCCTCPPPQVERPRGPRLGYSIPSRSGASG
  LDKRDYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020982
• ORF Size: 651 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020982.1, NM_020982.2, NM_020982.3, NP_066192.1
• RefSeq Size: 2139
• RefSeq ORF: 654
• Locus ID: 9080
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction
• MW: 22.8 kDa
• Gene Summary: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This protein is one of the entry cofactors for hepatitis C virus. Mouse studies revealed that this gene is required for the preservation of sensory cells in the hearing organ and the gene deficiency is associated with deafness. [provided by RefSeq, Jun 2010]