MTMR2 (NM_016156) Human Tagged ORF Clone

CAT#: RC208703

MTMR2 (Myc-DDK-tagged)-Human myotubularin related protein 2 (MTMR2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414151) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208703 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MTMR2 protein (Cat# TP308703). The protein was produced from HEK293T cells transfected with MTMR2 cDNA clone (Cat# RC208703) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTMR2
• Synonyms: CMT4B; CMT4B1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208703 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACGAGCTCGAGCTGCGAGAGTCTTGGCTCCCAGCCGGCGGCGGCTCGGCCGCCCAGCGTGGACT
  CCTTGTCCAGTGCCTCCACTTCTCATTCAGAGAATTCAGTGCATACAAAATCAGCTTCTGTTGTATCATC
  AGATTCCATTTCAACTTCTGCCGACAACTTTTCTCCTGATTTGAGGGTCCTGAGGGAGTCTAACAAGTTA
  GCAGAAATGGAAGAACCACCCTTGCTTCCAGGAGAAAATATTAAAGACATGGCCAAAGATGTAACTTATA
  TATGTCCATTCACTGGCGCTGTACGAGGAACTCTGACTGTCACGAATTATAGGTTATATTTCAAAAGCAT
  GGAACGGGATCCCCCATTTGTTTTAGATGCTTCCCTTGGTGTGATAAATAGAGTAGAAAAAATTGGTGGT
  GCTTCTAGTCGAGGTGAAAATTCTTATGGACTAGAAACTGTGTGTAAGGATATTAGGAATTTACGATTTG
  CTCATAAACCTGAGGGGCGGACAAGAAGATCCATATTTGAGAATCTAATGAAATATGCATTTCCTGTCTC
  TAATAACCTGCCTCTTTTTGCTTTTGAATACAAAGAAGTATTCCCTGAAAATGGGTGGAAGCTATATGAC
  CCTCTTTTAGAGTATAGAAGGCAGGGAATTCCAAATGAAAGCTGGAGAATAACAAAGATAAATGAACGAT
  ATGAACTTTGTGATACATACCCTGCCCTCCTGGTTGTGCCAGCAAATATTCCTGATGAAGAATTAAAGAG
  AGTGGCATCCTTCAGATCAAGAGGCCGTATCCCAGTTTTATCATGGATTCATCCTGAAAGTCAAGCCACA
  ATCACTCGGTGTAGCCAGCCCATGGTTGGAGTGAGTGGAAAGCGAAGCAAAGAAGATGAAAAATACCTTC
  AAGCTATCATGGATTCCAATGCCCAGTCTCACAAAATCTTTATATTTGATGCCCGGCCAAGTGTTAATGC
  TGTTGCCAACAAGGCAAAGGGTGGAGGTTATGAAAGTGAAGATGCCTATCAAAATGCTGAACTAGTTTTC
  CTGGATATCCACAATATTCATGTTATGAGAGAATCATTACGAAAACTTAAGGAGATTGTGTACCCCAACA
  TTGAGGAAACTCACTGGTTGTCTAACTTGGAATCTACTCATTGGCTAGAACATATTAAGCTTATTCTTGC
  AGGGGCTCTTAGGATTGCTGACAAGGTAGAGTCAGGGAAGACGTCTGTGGTAGTGCATTGCAGTGATGGT
  TGGGATCGCACAGCTCAGCTCACTTCCCTTGCCATGCTCATGTTGGATGGATACTATCGAACCATCCGAG
  GATTTGAAGTCCTTGTGGAGAAAGAATGGCTAAGTTTTGGACATCGATTTCAACTAAGAGTTGGCCATGG
  AGATAAGAACCATGCAGATGCAGACAGATCGCCTGTTTTTCTTCAATTTATTGACTGTGTCTGGCAGATG
  ACAAGACAGTTTCCTACCGCATTTGAATTCAATGAGTATTTTCTCATTACCATTTTGGACCACCTATACA
  GCTGCTTATTCGGAACATTCCTCTGTAATAGTGAACAACAGAGAGGAAAAGAGAATCTTCCTAAAAGGAC
  TGTGTCACTGTGGTCTTACATAAACAGCCAGCTGGAAGACTTCACTAATCCTCTCTATGGGAGCTATTCC
  AATCATGTCCTTTATCCAGTAGCCAGCATGCGCCACCTAGAGCTCTGGGTGGGATATTACATAAGGTGGA
  ATCCACGGATGAAACCACAGGAACCTATTCACAACAGATACAAAGAACTTCTTGCTAAACGAGCAGAGCT
  TCAGAAAAAAGTAGAGGAACTACAGAGAGAGATTTCTAACCGATCAACCTCATCCTCAGAGAGAGCCAGC
  TCTCCTGCACAGTGTGTCACTCCTGTCCAAACTGTTGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208703 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METSSSCESLGSQPAAARPPSVDSLSSASTSHSENSVHTKSASVVSSDSISTSADNFSPDLRVLRESNKL
  AEMEEPPLLPGENIKDMAKDVTYICPFTGAVRGTLTVTNYRLYFKSMERDPPFVLDASLGVINRVEKIGG
  ASSRGENSYGLETVCKDIRNLRFAHKPEGRTRRSIFENLMKYAFPVSNNLPLFAFEYKEVFPENGWKLYD
  PLLEYRRQGIPNESWRITKINERYELCDTYPALLVVPANIPDEELKRVASFRSRGRIPVLSWIHPESQAT
  ITRCSQPMVGVSGKRSKEDEKYLQAIMDSNAQSHKIFIFDARPSVNAVANKAKGGGYESEDAYQNAELVF
  LDIHNIHVMRESLRKLKEIVYPNIEETHWLSNLESTHWLEHIKLILAGALRIADKVESGKTSVVVHCSDG
  WDRTAQLTSLAMLMLDGYYRTIRGFEVLVEKEWLSFGHRFQLRVGHGDKNHADADRSPVFLQFIDCVWQM
  TRQFPTAFEFNEYFLITILDHLYSCLFGTFLCNSEQQRGKENLPKRTVSLWSYINSQLEDFTNPLYGSYS
  NHVLYPVASMRHLELWVGYYIRWNPRMKPQEPIHNRYKELLAKRAELQKKVEELQREISNRSTSSSERAS
  SPAQCVTPVQTVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016156
• ORF Size: 1929 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016156.1, NM_016156.2, NM_016156.3, NM_016156.4, NM_016156.5, NP_057240.3
• RefSeq Size: 4603 bp
• RefSeq ORF: 1932 bp
• Locus ID: 8898
• Domains: PTPc_motif, GRAM
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways, Riboflavin metabolism, Thiamine metabolism
• MW: 73.4 kDa
• Gene Summary: This gene is a member of the myotubularin family of phosphoinositide lipid phosphatases. The encoded protein possesses phosphatase activity towards phosphatidylinositol-3-phosphate and phosphatidylinositol-3,5-bisphosphate. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4B, an autosomal recessive demyelinating neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Aug 2011]