FANCL (NM_018062) Human Tagged ORF Clone

CAT#: RC208718

FANCL (Myc-DDK-tagged)-Human Fanconi anemia, complementation group L (FANCL), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FANCL
• Synonyms: FAAP43; PHF9; POG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208718 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGACGGAAGCGAGCCTGTTGCGCCAGTGCCCCCTGCTTCTGCCCCAGAACCGGTCGAAAACCG
  TGTATGAGGGATTCATCTCGGCTCAGGGAAGAGACTTCCACCTTAGGATAGTGTTGCCTGAAGATTTACA
  ACTGAAGAATGCAAGATTATTATGTAGTTGGCAGCTGAGAACAATACTTAGTGGATACCATCGAATAGTA
  CAACAGAGAATGCAGCACTCTCCTGATCTAATGAGCTTTATGATGGAGTTGAAGATGCTTTTGGAAGTTG
  CCTTAAAGAATAGACAAGAGCTGTATGCACTACCTCCTCCTCCCCAGTTCTACTCAAGCCTTATTGAAGA
  GATAGGAACTCTTGGTTGGGATAAACTTGTGTATGCGGATACCTGCTTCAGTACCATCAAGTTAAAAGCA
  GAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGAATCACCAG
  ATTATTTTGTGGATTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGAGCTCCTTAATAAGCATTTA
  TAGTCAGTTTTTGGCAGCAATAGAATCACTAAAGGCATTCTGGGATGTTATGGATGAAATCGATGAGAAG
  ACCTGGGTACTTGAGCCAGAAAAACCTCCACGGAGTGCAACAGCACGCAGAATTGCATTAGGTAATAATG
  TTTCCATAAATATAGAGGTAGACCCCAGGCATCCTACTATGCTTCCTGAGTGCTTCTTTCTTGGAGCTGA
  CCATGTGGTAAAACCCCTGGGAATTAAGCTGAGCAGGAACATACATTTGTGGGATCCAGAAAATAGTGTG
  TTACAAAATTTGAAAGATGTTTTAGAAATTGATTTTCCAGCTCGTGCTATCCTGGAAAAATCTGATTTTA
  CTATGGATTGTGGAATTTGTTATGCTTATCAACTTGACGGTACCATTCCTGATCAAGTGTGTGATAATTC
  CCAGTGTGGACAACCTTTCCATCAAATATGCTTATATGAGTGGCTGAGAGGACTACTAACTAGTAGACAG
  AGTTTTAACATCATATTTGGTGAATGTCCATATTGTAGTAAGCCAATTACCTTAAAAATGTCTGGAAGGA
  AACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208718 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVTEASLLRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVLPEDLQLKNARLLCSWQLRTILSGYHRIV
  QQRMQHSPDLMSFMMELKMLLEVALKNRQELYALPPPPQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKA
  EDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQFLAAIESLKAFWDVMDEIDEK
  TWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENSV
  LQNLKDVLEIDFPARAILEKSDFTMDCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQ
  SFNIIFGECPYCSKPITLKMSGRKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018062
• ORF Size: 1125 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018062.1, NM_018062.2, NM_018062.3, NP_060532.2
• RefSeq Size: 1738 bp
• RefSeq ORF: 1128 bp
• Locus ID: 55120
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 42.9 kDa
• Gene Summary: This gene encodes a ubiquitin ligase that is a member of the Fanconi anemia complementation group (FANC). Members of this group are related by their assembly into a common nuclear protein complex rather than by sequence similarity. This gene encodes the protein for complementation group L that mediates monoubiquitination of FANCD2 as well as FANCI. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2018]