FANCL (NM_018062) Human Untagged Clone

CAT#: SC113748

FANCL (untagged)-Human Fanconi anemia, complementation group L (FANCL), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FANCL
• Synonyms: FAAP43; PHF9; POG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113748 sequence for NM_018062 edited (data generated by NextGen Sequencing)
  ATGGCGGTGACGGAAGCGAGCCTGTTGCGCCAGTGCCCCCTGCTTCTGCCCCAGAACCGG
  TCGAAAACCGTGTATGAGGGATTCATCTCGGCTCAGGGAAGAGACTTCCACCTTAGGATA
  GTGTTGCCTGAAGATTTACAACTGAAGAATGCAAGATTATTATGTAGTTGGCAGCTGAGA
  ACAATACTTAGTGGATACCATCGAATAGTACAACAGAGAATGCAGCACTCTCCTGATCTA
  ATGAGCTTTATGATGGAGTTGAAGATGCTTTTGGAAGTTGCCTTAAAGAATAGACAAGAG
  CTGTATGCACTACCTCCTCCTCCCCAGTTCTACTCAAGCCTTATTGAAGAGATAGGAACT
  CTTGGTTGGGATAAACTTGTGTATGCGGATACCTGCTTCAGTACCATCAAGTTAAAAGCA
  GAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCA
  GAATCACCAGATTATTTTGTGGATTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAG
  AGCTCCTTAATAAGCATTTATAGTCAGTTTTTGGCAGCAATAGAATCACTAAAGGCATTC
  TGGGATGTTATGGATGAAATCGATGAGAAGACCTGGGTACTTGAGCCAGAAAAACCTCCA
  CGGAGTGCAACAGCACGCAGAATTGCATTAGGTAATAATGTTTCCATAAATATAGAGGTA
  GACCCCAGGCATCCTACTATGCTTCCTGAGTGCTTCTTTTTTGGAGCTGACCATGTGGTA
  AAACCCCTGGGAATTAAGCTGAGCAGGAACATACATTTGTGGGATCCAGAAAATAGTGTG
  TTACAAAATTTGAAAGATGTTTTAGAAATTGATTTTCCAGCTCGTGCTATCCTGGAAAAA
  TCTGATTTTACTATGGATTGTGGAATTTGTTATGCTTATCAACTTGACGGTACCATTCCT
  GATCAAGTGTGTGATAATTCCCAGTGTGGACAACCTTTCCATCAAATATGCTTATATGAG
  TGGCTGAGAGGACTACTAACTAGTAGACAGAGTTTTAACATCATATTTGGTGAATGTCCA
  TATTGTAGTAAGCCAATTACCTTAAAAATGTCTGGAAGGAAACACTGA
  
  Clone variation with respect to NM_018062.3
  760 c=>t;981 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018062 unedited
  NNGGGTGCACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCAGGTC
  TAGAGCTTTTCTGTGTTTCTCCGGACTTCGAGCCATGGCGGTGACGGAAGCGAGCCTGTT
  GCGCCAGTGCCCCCTGCTTCTGCCCCAGAACCGGTCGAAAACCGTGTATGAGGGATTCAT
  CTCGGCTCAGGGAAGAGACTTCCACCTTAGGATAGTGTTGCCTGAAGATTTACAACTGAA
  GAATGCAAGATTATTATGTAGTTGGCAGCTGAGAACAATACTTAGTGGATACCATCGAAT
  AGTACAACAGAGAATGCAGCACTCTCCTGATCTAATGAGCTTTATGATGGAGTTGAAGAT
  GCTTTTGGAAGTTGCCTTAAAGAATAGACAAGAGCTGTATGCACTACCTCCTCCTCCCCA
  GTTCTACTCAAGCCTTATTGAAGAGATAGGAACTCTTGGTTGGGATAAACTTGTGTATGC
  GGATACCTGCTTCAGTACCATCAAGTTAAAAGCAGAAGATGCTTCTGGTAGAGAGCATTT
  AATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGAATCACCAGATTATTTTGTGGATTT
  TCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGAGCTCCTTAATAAGCATTTATAGTCA
  GTTTTTGGCAGCAATAGAATCACTAAAGGCATTCTGGGATGTTATGGATGAAATCGATGA
  GAAGACCTGNGTACTTGAGCCAGANAAACCTCCACGGAGTGCAACAGCACGCAGAATTGC
  ATTAGGTAATAATGTTTCCATAAATATAGAGGTAGACCCCAGGCATNCTACTATGCTTNC
  TGAGTGCTTCTTTTTTGGAGCTGACCATGTGGTAAAACCCCTGNGAATTAAGCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018062 unedited
  ACGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGATCTTGTATAACATTT
  TATTTAGCATTCTTACACACTACACAAAATAAATACTTGGATAACTCACGTCTAACAAAC
  TAAACTATACTATGTATTCTTTCTCCATAGGGAAAGCACAAGGAGAAGACAGAAATATCA
  AGAGTCTCAAGAACCTTTGAATGAAGTAAACAGTTTCCCACAAAAAATCAGCTATACACA
  ATTCCCAAACTCATTTTATGAGCCTCATCAAGATTTTACCAGTCCAGATATATTCAAGAA
  GTCAAGATCTCCATCTTGGTATAAATACACTTCCACAGTCAGCACGGGGATCACAGACTT
  AGAAAGTTCAACTGGACTTTGGCCTACAATTTCCCAGTTTACTCTTAGTGAAGAGACAAA
  CGCAGATGTTTATTATTATCGCATCATCATACCTGTCCTTTTGATGTTAGTATTTCTTGC
  TTTATTTTTTCTCTGAAGATGATACCAAAATTCCTTTTGATAATTTTTTAAGTTTCCAGC
  TCTTCACCGAAATGTTGTATTCTTATTTCAGTGTTTCCTTCCAGACATTTTTAAGGTAAT
  TGGCTTACTACAATATGGACATTCACCAAATATGATGTTAAAACTCTGTCTACTAGTTAG
  TAGTCCTCTCAGCCACTCATATAAGCATATCTGATGGAAAGGCTGTCCACACTGGGAATT
  ATCACACACTTGATCAGGAATGGTACCGTCAAGTTGATAAGCCTAACAAATTCCACACTC
  CTATAAAATCAGATTTTCCAGATAGACGAGCTGGAAAATAATTTCTAAACATCTTCGAAT
  TTGCACACACTTTTTTTGAATCCACAAATGATGTGCTGCTCACTAATCCACAGGTTTACA
  CATGGCACCTCCAAAGAACCCCAGGACCTGTAGAGGCCGGGTCTACTT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_018062
• ORF Size: 1128 bp
• Insert Size: 1730
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_018062.2, NP_060532.2
• RefSeq Size: 1750
• RefSeq ORF: 1128
• Locus ID: 55120
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a ubiquitin ligase that is a member of the Fanconi anemia complementation group (FANC). Members of this group are related by their assembly into a common nuclear protein complex rather than by sequence similarity. This gene encodes the protein for complementation group L that mediates monoubiquitination of FANCD2 as well as FANCI. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2018]
  Transcript Variant: This variant (2) has an alternate splice site in the CDS, as compared to variant 1. The resulting isoform (2) lacks an internal segment and has identical N- and C-termini, as compared to isoform 1.