NUP62 (NM_153718) Human Tagged ORF Clone

CAT#: RC208727

NUP62 (Myc-DDK-tagged)-Human nucleoporin 62kDa (NUP62), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY413909) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210581 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NUP62
• Synonyms: IBSN; p62; SNDI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208727 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGGTTTAATTTTGGAGGCACTGGGGCCCCTACAGGCGGGTTCACGTTTGGCACTGCAAAGACGG
  CAACAACCACACCTGCTACAGGGTTTTCTTTCTCCACCTCTGGCACTGGAGGGTTTAATTTTGGGGCTCC
  CTTCCAACCAGCCACAAGTACCCCTTCCACCGGCCTGTTCTCACTTGCCACCCAGACTCCGGCCACACAG
  ACGACAGGCTTCACTTTTGGAACAGCGACTCTTGCTTCGGGGGGAACTGGATTTTCTTTGGGGATCGGTG
  CTTCAAAGCTCAACTTGAGCAACACAGCTGCCACCCCAGCCATGGCAAACCCCAGCGGCTTTGGGCTGGG
  CAGCAGCAACCTCACTAATGCCATATCGAGCACCGTCACCTCCAGCCAGGGCACAGCACCCACCGGCTTT
  GTGTTTGGCCCCTCCACCACCTCTGTGGCTCCAGCTACCACATCTGGAGGCTTCTCATTCACTGGTGGAA
  GCACGGCCCAACCCTCCGGTTTCAACATTGGCTCAGCAGGGAATTCAGCCCAGCCCACGGCACCTGCCAC
  GTTGCCCTTCACTCCGGCCACGCCAGCAGCCACCACAGCAGGTGCCACACAGCCAGCTGCTCCCACACCC
  ACAGCCACCATCACCAGCACTGGGCCCAGCCTCTTTGCGTCAATAGCAACTGCTCCAACCTCATCTGCCA
  CCACTGGACTCTCCCTCTGTACCCCTGTGACCACAGCGGGCGCCCCCACTGCTGGGACACAGGGCTTCAG
  CTTAAAGGCACCTGGAGCAGCTTCCGGCACCTCCACAACAACATCCACCGCTGCCACCGCCACCGCCACC
  ACCACCAGCAGCAGCAGCACCACCGGCTTTGCCTTGAATTTAAAACCACTGGCGCCAGCCGGGATCCCCA
  GCAATACAGCAGCTGCCGTGACCGCTCCACCTGGCCCTGGCGCAGCTGCAGGGGCGGCTGCCAGCTCCGC
  CATGACCTACGCGCAGCTGGAGAGCCTGATCAACAAATGGAGCCTGGAGCTAGAGGACCAGGAGCGGCAC
  TTCCTCCAGCAGGCCACCCAGGTCAACGCCTGGGACCGCACGCTGATCGAGAATGGAGAAAAGATCACCA
  GCCTGCACCGCGAGGTGGAGAAGGTGAAGCTGGACCAGAAGAGGCTGGACCAGGAGCTCGACTTCATCCT
  GTCCCAGCAGAAGGAGCTGGAAGACCTGCTGAGCCCACTGGAGGAGTTGGTCAAGGAGCAGAGCGGGACC
  ATCTACCTGCAGCACGCGGATGAGGAGCGTGAGAAAACCTACAAGCTGGCTGAGAACATCGACGCACAGC
  TCAAGCGCATGGCCCAGGATCTCAAGGACATCATCGAGCACCTGAACACGTCCGGGGCCCCCGCCGACAC
  CAGTGACCCACTGCAGCAGATCTGCAAGATCCTCAATGCGCACATGGACTCACTGCAGTGGATCGACCAG
  AACTCGGCCCTGCTGCAGAGGAAGGTGGAGGAGGTGACCAAGGTGTGCGAGGGCCGGCGCAAGGAGCAGG
  AGCGCAGCTTCCGGATCACCTTTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208727 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGFNFGGTGAPTGGFTFGTAKTATTTPATGFSFSTSGTGGFNFGAPFQPATSTPSTGLFSLATQTPATQ
  TTGFTFGTATLASGGTGFSLGIGASKLNLSNTAATPAMANPSGFGLGSSNLTNAISSTVTSSQGTAPTGF
  VFGPSTTSVAPATTSGGFSFTGGSTAQPSGFNIGSAGNSAQPTAPATLPFTPATPAATTAGATQPAAPTP
  TATITSTGPSLFASIATAPTSSATTGLSLCTPVTTAGAPTAGTQGFSLKAPGAASGTSTTTSTAATATAT
  TTSSSSTTGFALNLKPLAPAGIPSNTAAAVTAPPGPGAAAGAAASSAMTYAQLESLINKWSLELEDQERH
  FLQQATQVNAWDRTLIENGEKITSLHREVEKVKLDQKRLDQELDFILSQQKELEDLLSPLEELVKEQSGT
  IYLQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQ
  NSALLQRKVEEVTKVCEGRRKEQERSFRITFD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153718
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153718.1, NM_153718.2, NM_153718.3, NP_714940.1
• RefSeq Size: 3280 bp
• RefSeq ORF: 1569 bp
• Locus ID: 23636
• Domains: Nsp1_C
• Protein Families: Druggable Genome, Transcription Factors
• MW: 53.3 kDa
• Gene Summary: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq, Jul 2008]