NUP62 (NM_153718) Human Tagged ORF Clone
CAT#: RC208727
NUP62 (Myc-DDK-tagged)-Human nucleoporin 62kDa (NUP62), transcript variant 3
"NM_153718" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NUP62 |
Synonyms | IBSN; p62; SNDI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC208727 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGGTTTAATTTTGGAGGCACTGGGGCCCCTACAGGCGGGTTCACGTTTGGCACTGCAAAGACGG CAACAACCACACCTGCTACAGGGTTTTCTTTCTCCACCTCTGGCACTGGAGGGTTTAATTTTGGGGCTCC CTTCCAACCAGCCACAAGTACCCCTTCCACCGGCCTGTTCTCACTTGCCACCCAGACTCCGGCCACACAG ACGACAGGCTTCACTTTTGGAACAGCGACTCTTGCTTCGGGGGGAACTGGATTTTCTTTGGGGATCGGTG CTTCAAAGCTCAACTTGAGCAACACAGCTGCCACCCCAGCCATGGCAAACCCCAGCGGCTTTGGGCTGGG CAGCAGCAACCTCACTAATGCCATATCGAGCACCGTCACCTCCAGCCAGGGCACAGCACCCACCGGCTTT GTGTTTGGCCCCTCCACCACCTCTGTGGCTCCAGCTACCACATCTGGAGGCTTCTCATTCACTGGTGGAA GCACGGCCCAACCCTCCGGTTTCAACATTGGCTCAGCAGGGAATTCAGCCCAGCCCACGGCACCTGCCAC GTTGCCCTTCACTCCGGCCACGCCAGCAGCCACCACAGCAGGTGCCACACAGCCAGCTGCTCCCACACCC ACAGCCACCATCACCAGCACTGGGCCCAGCCTCTTTGCGTCAATAGCAACTGCTCCAACCTCATCTGCCA CCACTGGACTCTCCCTCTGTACCCCTGTGACCACAGCGGGCGCCCCCACTGCTGGGACACAGGGCTTCAG CTTAAAGGCACCTGGAGCAGCTTCCGGCACCTCCACAACAACATCCACCGCTGCCACCGCCACCGCCACC ACCACCAGCAGCAGCAGCACCACCGGCTTTGCCTTGAATTTAAAACCACTGGCGCCAGCCGGGATCCCCA GCAATACAGCAGCTGCCGTGACCGCTCCACCTGGCCCTGGCGCAGCTGCAGGGGCGGCTGCCAGCTCCGC CATGACCTACGCGCAGCTGGAGAGCCTGATCAACAAATGGAGCCTGGAGCTAGAGGACCAGGAGCGGCAC TTCCTCCAGCAGGCCACCCAGGTCAACGCCTGGGACCGCACGCTGATCGAGAATGGAGAAAAGATCACCA GCCTGCACCGCGAGGTGGAGAAGGTGAAGCTGGACCAGAAGAGGCTGGACCAGGAGCTCGACTTCATCCT GTCCCAGCAGAAGGAGCTGGAAGACCTGCTGAGCCCACTGGAGGAGTTGGTCAAGGAGCAGAGCGGGACC ATCTACCTGCAGCACGCGGATGAGGAGCGTGAGAAAACCTACAAGCTGGCTGAGAACATCGACGCACAGC TCAAGCGCATGGCCCAGGATCTCAAGGACATCATCGAGCACCTGAACACGTCCGGGGCCCCCGCCGACAC CAGTGACCCACTGCAGCAGATCTGCAAGATCCTCAATGCGCACATGGACTCACTGCAGTGGATCGACCAG AACTCGGCCCTGCTGCAGAGGAAGGTGGAGGAGGTGACCAAGGTGTGCGAGGGCCGGCGCAAGGAGCAGG AGCGCAGCTTCCGGATCACCTTTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208727 protein sequence
Red=Cloning site Green=Tags(s) MSGFNFGGTGAPTGGFTFGTAKTATTTPATGFSFSTSGTGGFNFGAPFQPATSTPSTGLFSLATQTPATQ TTGFTFGTATLASGGTGFSLGIGASKLNLSNTAATPAMANPSGFGLGSSNLTNAISSTVTSSQGTAPTGF VFGPSTTSVAPATTSGGFSFTGGSTAQPSGFNIGSAGNSAQPTAPATLPFTPATPAATTAGATQPAAPTP TATITSTGPSLFASIATAPTSSATTGLSLCTPVTTAGAPTAGTQGFSLKAPGAASGTSTTTSTAATATAT TTSSSSTTGFALNLKPLAPAGIPSNTAAAVTAPPGPGAAAGAAASSAMTYAQLESLINKWSLELEDQERH FLQQATQVNAWDRTLIENGEKITSLHREVEKVKLDQKRLDQELDFILSQQKELEDLLSPLEELVKEQSGT IYLQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQ NSALLQRKVEEVTKVCEGRRKEQERSFRITFD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153718 |
ORF Size | 1566 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153718.1, NM_153718.2, NM_153718.3, NP_714940.1 |
RefSeq Size | 3280 bp |
RefSeq ORF | 1569 bp |
Locus ID | 23636 |
Domains | Nsp1_C |
Protein Families | Druggable Genome, Transcription Factors |
MW | 53.3 kDa |
Gene Summary | The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC321889 | NUP62 (untagged)-Human nucleoporin 62kDa (NUP62), transcript variant 3 |
USD 760.00 |
|
RG208727 | NUP62 (GFP-tagged) - Human nucleoporin 62kDa (NUP62), transcript variant 3 |
USD 530.00 |
|
RC208727L3 | Lenti ORF clone of Human nucleoporin 62kDa (NUP62), transcript variant 3, Myc-DDK-tagged |
USD 680.00 |
|
RC208727L4 | Lenti ORF clone of Human nucleoporin 62kDa (NUP62), transcript variant 3, mGFP tagged |
USD 680.00 |
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