LEPROT (NM_017526) Human Tagged ORF Clone
CAT#: RC208878
LEPROT (Myc-DDK-tagged)-Human leptin receptor overlapping transcript (LEPROT), transcript variant 1
"NM_017526" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | LEPROT |
Synonyms | LEPR; OB-RGRP; OBRGRP; VPS55 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC208878 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGTTAAAGCTCTCGTGGCATTATCCTTCAGTGGGGCTATTGGACTGACTTTTCTTATGCTGG GATGTGCCTTAGAGGATTATGGCGTTTACTGGCCCTTATTCGTCCTGATTTTCCACGCCATCTCCCCCAT CCCCCATTTCATTGCCAAAAGAGTCACCTATGACTCAGATGCAACCAGTAGTGCCTGTCGGGAACTGGCA TATTTCTTCACTACTGGAATTGTTGTTTCTGCCTTTGGATTTCCTGTTATTCTTGCTCGTGTGGCTGTGA TCAAATGGGGAGCCTGCGGCCTTGTGTTGGCAGGCAATGCAGTCATTTTCCTTACAATTCAAGGGTTTTT CCTTATATTTGGAAGAGGAGATGATTTTAGCTGGGAGCAGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208878 protein sequence
Red=Cloning site Green=Tags(s) MAGVKALVALSFSGAIGLTFLMLGCALEDYGVYWPLFVLIFHAISPIPHFIAKRVTYDSDATSSACRELA YFFTTGIVVSAFGFPVILARVAVIKWGACGLVLAGNAVIFLTIQGFFLIFGRGDDFSWEQW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017526 |
ORF Size | 393 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017526.1, NM_017526.2, NM_017526.3, NM_017526.4, NP_059996.1 |
RefSeq Size | 4777 bp |
RefSeq ORF | 396 bp |
Locus ID | 54741 |
Domains | Vps55 |
Protein Families | Druggable Genome, Transmembrane |
MW | 14.3 kDa |
Gene Summary | LEPROT is associated with the Golgi complex and endosomes and has a role in cell surface expression of growth hormone receptor (GHR; MIM 600946) and leptin receptor (OBR, or LEPR; MIM 601007), thereby altering receptor-mediated cell signaling (Couturier et al., 2007 [PubMed 18042720]; Touvier et al., 2009 [PubMed 19907080]). [supplied by OMIM, Jul 2010] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC127307 | LEPROT (untagged)-Human leptin receptor overlapping transcript (LEPROT), transcript variant 1 |
USD 310.00 |
|
RG208878 | LEPROT (GFP-tagged) - Human leptin receptor overlapping transcript (LEPROT), transcript variant 1 |
USD 460.00 |
|
RC208878L1 | Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC208878L2 | Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC208878L3 | Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC208878L4 | Lenti ORF clone of Human leptin receptor overlapping transcript (LEPROT), transcript variant 1, mGFP tagged |
USD 620.00 |
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