USP38 (NM_032557) Human Tagged ORF Clone

CAT#: RC208974

USP38 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 38 (USP38)

Product Images

Western blot validation of overexpression lysate (Cat# LY410045) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208974 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified USP38 protein (Cat# TP308974). The protein was produced from HEK293T cells transfected with USP38 cDNA clone (Cat# RC208974) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP38
• Synonyms: HP43.8KD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208974 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGATCCTGGAGGGCCTTGTGAGTTCCTCGCATCCCCTGCCCCTCAAGCGGGTGATTGTGCGGA
  AGGTGGTGGAATCGGCGGAGCACTGGCTAGACGAGGCGCAGTGCGAGGCCATGTTTGACCTGACGACCCG
  GCTCATCCTGGAGGGCCAGGACCCTTTCCAGCGGCAGGTGGGGCACCAGGTGCTGGAGGCCTACGCACGA
  TACCACCGGCCAGAGTTCGAGTCCTTCTTCAACAAGACCTTCGTGTTGGGCCTCCTTCATCAGGGCTACC
  ACTCTCTGGACAGGAAGGATGTAGCCATCCTGGACTACATTCACAACGGCCTGAAGCTGATTATGAGCTG
  TCCGTCGGTGCTGGATCTCTTTAGCCTCCTGCAGGTAGAGGTGTTACGGATGGTGTGTGAGAGGCCGGAG
  CCGCAGCTCTGTGCCCGACTGAGCGACCTTCTGACCGACTTTGTGCAATGCATCCCCAAGGGGAAATTGT
  CCATCACGTTCTGTCAACAGCTGGTTCGAACGATAGGCCATTTCCAGTGCGTGTCCACCCAGGAAAGAGA
  GCTGCGGGAATATGTCTCCCAGGTGACAAAAGTGAGTAACTTGCTGCAGAACATCTGGAAGGCCGAGCCT
  GCCACACTACTGCCTTCCCTGCAAGAAGTTTTTGCAAGCATCTCTTCCACAGATGCATCATTTGAACCTT
  CTGTAGCATTGGCAAGCCTTGTGCAGCATATTCCTCTTCAGATGATTACAGTTCTCATCAGGAGCCTTAC
  TACGGATCCAAATGTAAAAGATGCAAGTATGACCCAAGCCCTTTGCAGAATGATTGACTGGCTATCCTGG
  CCATTGGCTCAGCATGTGGATACATGGGTAATTGCACTCCTGAAAGGACTGGCAGCTGTCCAGAAGTTTA
  CTATTTTGATAGATGTTACTTTGCTGAAAATAGAACTGGTTTTTAATCGACTTTGGTTTCCTCTTGTGAG
  ACCTGGTGCTCTTGCAGTTCTTTCTCACATGCTGCTTAGCTTTCAGCATTCTCCAGAGGCGTTCCATTTG
  ATTGTTCCTCATGTGGTTAATTTGGTTCATTCTTTCAAAAATGATGGTCTGCCTTCAAGTACAGCCTTCT
  TAGTACAATTAACAGAATTGATACACTGTATGATGTATCATTATTCTGGATTTCCAGATCTCTATGAACC
  TATTCTGGAGGCAATAAAGGATTTTCCTAAGCCCAGTGAAGAGAAGATTAAGTTAATTCTCAATCAAAGT
  GCCTGGACTTCTCAATCCAATTCTTTGGCGTCTTGCTTGTCTAGACTTTCTGGAAAATCTGAAACTGGGA
  AAACTGGTCTTATTAACCTAGGAAATACATGTTATATGAACAGTGTTATACAAGCCTTGTTTATGGCCAC
  AGATTTCAGGAGACAAGTATTATCTTTAAATCTAAACGGGTGCAATTCATTAATGAAAAAATTACAGCAT
  CTTTTTGCCTTTCTGGCCCATACACAGAGGGAAGCATACGCACCTCGGATATTCTTTGAGGCTTCCAGAC
  CTCCATGGTTTACTCCCAGATCACAGCAAGACTGTTCTGAATACCTCAGATTTCTCCTTGACAGGCTCCA
  TGAAGAAGAAAAGATCTTGAAAGTTCAGGCCTCACACAAGCCTTCTGAAATTCTGGAATGCAGTGAAACT
  TCTTTACAGGAAGTAGCTAGTAAAGCAGCAGTACTAACAGAGACCCCTCGTACAAGTGACGGTGAGAAGA
  CTTTAATAGAAAAAATGTTTGGAGGAAAACTACGAACTCACATACGTTGTTTGAACTGCAGGAGTACCTC
  ACAAAAAGTGGAAGCCTTTACAGATCTTTCGCTTGCCTTTTGTCCTTCCTCTTCTTTGGAAAACATGTCT
  GTCCAAGATCCAGCATCATCACCCAGTATACAAGATGGTGGTCTAATGCAAGCCTCTGTACCCGGTCCTT
  CAGAAGAACCAGTAGTTTATAATCCAACAACAGCTGCCTTCATCTGTGACTCACTTGTGAATGAAAAAAC
  CATAGGCAGTCCTCCTAATGAGTTTTACTGTTCTGAAAACACTTCTGTCCCTAACGAATCTAACAAGATT
  CTTGTTAATAAAGATGTACCTCAGAAACCAGGAGGTGAAACCACACCTTCAGTAACTGACTTACTAAATT
  ATTTTTTGGCTCCAGAGATTCTTACTGGTGATAACCAATATTATTGTGAAAACTGTGCCTCTCTGCAAAA
  TGCTGAGAAAACTATGCAAATCACGGAGGAACCTGAATACCTTATTCTTACTCTCCTGAGATTTTCATAT
  GATCAGAAGTATCATGTGAGAAGGAAAATTTTAGACAATGTATCACTGCCACTGGTTTTGGAGTTGCCAG
  TTAAAAGAATTACTTCTTTCTCTTCATTGTCAGAAAGTTGGTCTGTAGATGTTGACTTCACTGATCTTAG
  TGAGAACCTTGCTAAAAAATTAAAGCCTTCAGGGACTGATGAAGCTTCCTGCACAAAATTGGTGCCCTAT
  CTATTAAGTTCCGTTGTGGTTCACTCTGGTATATCCTCTGAAAGTGGGCATTACTATTCTTATGCCAGAA
  ATATCACAAGTACAGACTCTTCATATCAGATGTACCACCAGTCTGAGGCTCTGGCATTAGCATCCTCCCA
  GAGTCATTTACTAGGGAGAGATAGTCCCAGTGCAGTTTTTGAACAGGATTTGGAAAATAAGGAAATGTCA
  AAAGAATGGTTTTTATTTAATGACAGTAGAGTGACATTTACTTCATTTCAGTCAGTCCAGAAAATTACGA
  GCAGGTTTCCAAAGGACACAGCTTATGTGCTTTTGTATAAAAAACAGCATAGTACTAATGGTTTAAGTGG
  TAATAACCCAACCAGTGGACTCTGGATAAATGGAGACCCACCTCTACAGAAAGAACTTATGGATGCTATA
  ACAAAAGACAATAAACTATATTTACAGGAACAAGAGTTGAATGCTCGAGCCCGGGCCCTCCAAGCTGCAT
  CTGCTTCATGTTCATTTCGGCCCAATGGATTTGATGACAACGACCCACCAGGAAGCTGTGGACCAACTGG
  TGGAGGGGGTGGAGGAGGATTTAATACAGTTGGCAGACTCGTATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208974 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDKILEGLVSSSHPLPLKRVIVRKVVESAEHWLDEAQCEAMFDLTTRLILEGQDPFQRQVGHQVLEAYAR
  YHRPEFESFFNKTFVLGLLHQGYHSLDRKDVAILDYIHNGLKLIMSCPSVLDLFSLLQVEVLRMVCERPE
  PQLCARLSDLLTDFVQCIPKGKLSITFCQQLVRTIGHFQCVSTQERELREYVSQVTKVSNLLQNIWKAEP
  ATLLPSLQEVFASISSTDASFEPSVALASLVQHIPLQMITVLIRSLTTDPNVKDASMTQALCRMIDWLSW
  PLAQHVDTWVIALLKGLAAVQKFTILIDVTLLKIELVFNRLWFPLVRPGALAVLSHMLLSFQHSPEAFHL
  IVPHVVNLVHSFKNDGLPSSTAFLVQLTELIHCMMYHYSGFPDLYEPILEAIKDFPKPSEEKIKLILNQS
  AWTSQSNSLASCLSRLSGKSETGKTGLINLGNTCYMNSVIQALFMATDFRRQVLSLNLNGCNSLMKKLQH
  LFAFLAHTQREAYAPRIFFEASRPPWFTPRSQQDCSEYLRFLLDRLHEEEKILKVQASHKPSEILECSET
  SLQEVASKAAVLTETPRTSDGEKTLIEKMFGGKLRTHIRCLNCRSTSQKVEAFTDLSLAFCPSSSLENMS
  VQDPASSPSIQDGGLMQASVPGPSEEPVVYNPTTAAFICDSLVNEKTIGSPPNEFYCSENTSVPNESNKI
  LVNKDVPQKPGGETTPSVTDLLNYFLAPEILTGDNQYYCENCASLQNAEKTMQITEEPEYLILTLLRFSY
  DQKYHVRRKILDNVSLPLVLELPVKRITSFSSLSESWSVDVDFTDLSENLAKKLKPSGTDEASCTKLVPY
  LLSSVVVHSGISSESGHYYSYARNITSTDSSYQMYHQSEALALASSQSHLLGRDSPSAVFEQDLENKEMS
  KEWFLFNDSRVTFTSFQSVQKITSRFPKDTAYVLLYKKQHSTNGLSGNNPTSGLWINGDPPLQKELMDAI
  TKDNKLYLQEQELNARARALQAASASCSFRPNGFDDNDPPGSCGPTGGGGGGGFNTVGRLVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032557
• ORF Size: 3126 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032557.2, NM_032557.3, NM_032557.4, NM_032557.5, NM_032557.6, NP_115946.2
• RefSeq Size: 7081 bp
• RefSeq ORF: 3129 bp
• Locus ID: 84640
• Domains: UCH
• Protein Families: Druggable Genome, Protease
• MW: 116.5 kDa
• Gene Summary: Deubiquitinating enzyme exhibiting a preference towards 'Lys-63'-linked ubiquitin chains. [UniProtKB/Swiss-Prot Function]