KREMEN1 (NM_001039571) Human Tagged ORF Clone

CAT#: RC208991

KREMEN1 (Myc-DDK-tagged)-Human kringle containing transmembrane protein 1 (KREMEN1), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY422074) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208991 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KREMEN1 protein (Cat# TP308991). The protein was produced from HEK293T cells transfected with KREMEN1 cDNA clone (Cat# RC208991) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KREMEN1
• Synonyms: FLJ31863; KREMEM1; KREMEN; kringle-coding gene marking the eye and the nose; kringle-containing transmembrane protein 1; kringle containing transmembrane protein 1; KRM1; OTTHUMP00000028977
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208991 representing NM_001039571
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGCCCGCCGCCCGCCTCGCCCTGCTCTCCGCCGCGGCGCTCACGCTGGCGGCCCGGCCCGCGC
  CTAGCCCCGGCCTCGGCCCCGGACCCGAGTGTTTCACAGCCAATGGTGCGGATTATAGGGGAACACAGAA
  CTGGACAGCACTACAAGGCGGGAAGCCATGTCTGTTTTGGAACGAGACTTTCCAGCATCCATACAACACT
  CTGAAATACCCCAACGGGGAGGGGGGCCTGGGTGAGCACAACTATTGCAGAAATCCAGATGGAGACGTGA
  GCCCCTGGTGCTATGTGGCAGAGCACGAGGATGGTGTCTACTGGAAGTACTGTGAGATACCTGCTTGCCA
  GATGCCTGGAAACCTTGGCTGCTACAAGGATCATGGAAACCCACCTCCTCTAACTGGCACCAGTAAAACG
  TCCAACAAACTCACCATACAAACTTGCATCAGTTTTTGTCGGAGTCAGAGGTTCAAGTTTGCTGGGATGG
  AGTCAGGCTATGCTTGCTTCTGTGGAAACAATCCTGATTACTGGAAGTACGGGGAGGCAGCCAGTACCGA
  ATGCAACAGCGTCTGCTTCGGGGATCACACCCAACCCTGTGGTGGCGATGGCAGGATCATCCTCTTTGAT
  ACTCTCGTGGGCGCCTGCGGTGGGAACTACTCAGCCATGTCTTCTGTGGTCTATTCCCCTGACTTCCCCG
  ACACCTATGCCACGGGGAGGGTCTGCTACTGGACCATCCGGGTTCCGGGGGCCTCCCACATCCACTTCAG
  CTTCCCCCTATTTGACATCAGGGACTCGGCGGACATGGTGGAGCTTCTGGATGGCTACACCCACCGTGTC
  CTAGCCCGCTTCCACGGGAGGAGCCGCCCACCTCTGTCCTTCAACGTCTCTCTGGACTTCGTCATCTTGT
  ATTTCTTCTCTGATCGCATCAATCAGGCCCAGGGATTTGCTGTTTTATACCAAGCCGTCAAGGAAGAACT
  GCCACAGGAGAGGCCCGCTGTCAACCAGACGGTGGCCGAGGTGATCACGGAGCAGGCCAACCTCAGTGTC
  AGCGCTGCCCGGTCCTCCAAAGTCCTCTATGTCATCACCACCAGCCCCAGCCACCCACCTCAGACTGTCC
  CAGGATGGACAGTCTATGGTCTGGCAACTCTCCTCATCCTCACAGTCACAGCCATTGTAGCAAAGATACT
  TCTGCACGTCACATTCAAATCCCATCGTGTTCCTGCTTCAGGGGACCTTAGGGATTGTCATCAACCAGGG
  ACTTCGGGGGAAATCTGGAGCATTTTTTACAAGCCTTCCACTTCAATTTCCATCTTTAAGAAGAAACTCA
  AGGGTCAGAGTCAACAAGATGACCGCAATCCCCTTGTGAGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208991 representing NM_001039571
  Red=Cloning site Green=Tags(s)
  
  MAPPAARLALLSAAALTLAARPAPSPGLGPGPECFTANGADYRGTQNWTALQGGKPCLFWNETFQHPYNT
  LKYPNGEGGLGEHNYCRNPDGDVSPWCYVAEHEDGVYWKYCEIPACQMPGNLGCYKDHGNPPPLTGTSKT
  SNKLTIQTCISFCRSQRFKFAGMESGYACFCGNNPDYWKYGEAASTECNSVCFGDHTQPCGGDGRIILFD
  TLVGACGGNYSAMSSVVYSPDFPDTYATGRVCYWTIRVPGASHIHFSFPLFDIRDSADMVELLDGYTHRV
  LARFHGRSRPPLSFNVSLDFVILYFFSDRINQAQGFAVLYQAVKEELPQERPAVNQTVAEVITEQANLSV
  SAARSSKVLYVITTSPSHPPQTVPGWTVYGLATLLILTVTAIVAKILLHVTFKSHRVPASGDLRDCHQPG
  TSGEIWSIFYKPSTSISIFKKKLKGQSQQDDRNPLVSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039571
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039571.1, NP_001034660.1
• RefSeq Size: 6115
• RefSeq ORF: 1376
• Locus ID: 83999
• Protein Families: Druggable Genome, Transmembrane
• MW: 50.18 kDa
• Gene Summary: This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq, Jul 2008]