Pinin (PNN) (NM_002687) Human Tagged ORF Clone

CAT#: RC209069

PNN (Myc-DDK-tagged)-Human pinin, desmosome associated protein (PNN)

Product Images

Western blot validation of overexpression lysate (Cat# LY419166) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209069 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PNN
• Synonyms: DRS; DRSP; memA; SDK3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209069 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTCGCCGTAAGAACTTTGCAGGAACAGCTGGAAAAGGCCAAAGAGAGTCTTAAGAACGTGGATG
  AGAACATTCGCAAGCTCACCGGGCGGGATCCGAATGACGTGAGGCCCATCCAAGCCAGATTGCTGGCCCT
  TTCTGGTCCTGGTGGAGGTAGAGGACGTGGTAGTTTATTACTGAGGCGTGGATTCTCAGATAGTGGAGGA
  GGACCCCCAGCCAAACAGAGAGACCTTGAAGGGGCAGTCAGTAGGCTGGGCGGGGAGCGTCGGACCAGAA
  GAGAATCACGCCAGGAAAGCGACCCGGAGGATGATGATGTTAAAAAGCCAGCATTGCAGTCTTCAGTTGT
  AGCTACCTCCAAAGAGCGCACACGTAGAGACCTTATCCAGGATCAAAATATGGATGAAAAGGGAAAGCAA
  AGGAACCGGCGAATATTTGGCTTGTTGATGGGTACCCTTCAAAAATTTAAACAAGAATCCACTGTTGCTA
  CTGAAAGGCAAAAGCGGCGCCAGGAAATTGAACAAAAACTTGAAGTTCAGGCAGAAGAAGAGAGAAAGCA
  GGTTGAAAATGAAAGGAGAGAACTGTTTGAAGAGAGGCGTGCTAAACAGACAGAACTGCGGCTTTTGGAA
  CAGAAAGTTGAGCTTGCGCAGCTGCAAGAAGAATGGAATGAACATAATGCCAAAATAATTAAATATATAA
  GAACTAAGACAAAGCCCCATTTGTTTTATATTCCTGGAAGAATGTGTCCAGCTACCCAAAAACTAATAGA
  AGAGTCACAGAGAAAAATGAACGCTTTATTTGAAGGTAGACGCATCGAATTTGCAGAACAAATAAATAAA
  ATGGAGGCTAGGCCTAGAAGACAATCAATGAAGGAAAAAGAGCATCAGGTGGTGCGTAATGAAGAACAGA
  AGGCGGAACAAGAAGAGGGTAAGGTGGCTCAGCGAGAGGAAGAGTTGGAGGAGACAGGTAATCAGCACAA
  TGATGTAGAAATAGAGGAAGCAGGAGAGGAAGAGGAAAAGGAAATAGCGATTGTTCATAGTGATGCAGAG
  AAAGAACAGGAGGAGGAAGAACAAAAACAGGAAATGGAGGTTAAGATGGAGGAGGAAACTGAGGTAAGGG
  AAAGTGAGAAGCAGCAGGATAGTCAGCCTGAAGAAGTTATGGATGTGCTAGAGATGGTTGAGAATGTCAA
  ACATGTAATTGCTGACCAGGAGGTAATGGAAACTAATCGAGTTGAAAGTGTAGAACCTTCAGAAAATGAA
  GCTAGCAAAGAATTGGAACCAGAAATGGAATTTGAAATTGAGCCAGATAAAGAATGTAAATCCCTTTCTC
  CTGGGAAAGAGAATGTCAGTGCTTTAGACATGGAAAAGGAGTCTGAGGAAAAAGAAGAAAAAGAATCTGA
  GCCCCAACCTGAGCCTGTGGCTCAACCTCAGCCTCAGTCTCAGCCCCAGCTTCAGCTTCAATCCCAGTCC
  CAACCAGTACTCCAGTCCCAGCCTCCCTCTCAGCCTGAGGATTTGTCATTAGCTGTTTTACAGCCAACAC
  CCCAAGTTACTCAGGAGCAAGGGCATTTACTACCTGAGAGGAAGGATTTTCCTGTAGAGTCTGTAAAACT
  CACTGAGGTACCAGTAGAGCCAGTCTTGACAGTACATCCAGAGAGCAAGAGCAAAACCAAAACTAGGAGC
  AGAAGTAGAGGTCGAGCTAGAAATAAAACAAGCAAGAGTAGAAGTCGAAGCAGTAGCAGTAGCAGTTCTA
  GTAGCAGTTCAACCAGTAGCAGCAGTGGAAGTAGTTCCAGCAGTGGAAGTAGTAGCAGTCGCAGTAGTTC
  CAGTAGCAGCTCCAGTACAAGTGGCAGCAGCAGCAGAGATAGTAGCAGTAGCACTAGTAGTAGTAGTGAG
  AGTAGAAGTCGGAGTAGGGGCCGGGGACATAATAGAGATAGAAAGCACAGAAGGAGCGTGGATCGGAAGA
  GAAGGGATACTTCAGGACTAGAAAGAAGTCACAAATCTTCAAAAGGTGGTAGTAGTAGAGATACAAAAGG
  ATCAAAGGATAAGAATTCCCGGTCCGACAGAAAGAGGTCTATATCAGAGAGTAGTCGATCAGGCAAAAGA
  TCTTCAAGAAGTGAAAGAGACCGAAAATCAGACAGGAAAGACAAAAGGCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209069 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVAVRTLQEQLEKAKESLKNVDENIRKLTGRDPNDVRPIQARLLALSGPGGGRGRGSLLLRRGFSDSGG
  GPPAKQRDLEGAVSRLGGERRTRRESRQESDPEDDDVKKPALQSSVVATSKERTRRDLIQDQNMDEKGKQ
  RNRRIFGLLMGTLQKFKQESTVATERQKRRQEIEQKLEVQAEEERKQVENERRELFEERRAKQTELRLLE
  QKVELAQLQEEWNEHNAKIIKYIRTKTKPHLFYIPGRMCPATQKLIEESQRKMNALFEGRRIEFAEQINK
  MEARPRRQSMKEKEHQVVRNEEQKAEQEEGKVAQREEELEETGNQHNDVEIEEAGEEEEKEIAIVHSDAE
  KEQEEEEQKQEMEVKMEEETEVRESEKQQDSQPEEVMDVLEMVENVKHVIADQEVMETNRVESVEPSENE
  ASKELEPEMEFEIEPDKECKSLSPGKENVSALDMEKESEEKEEKESEPQPEPVAQPQPQSQPQLQLQSQS
  QPVLQSQPPSQPEDLSLAVLQPTPQVTQEQGHLLPERKDFPVESVKLTEVPVEPVLTVHPESKSKTKTRS
  RSRGRARNKTSKSRSRSSSSSSSSSSSTSSSSGSSSSSGSSSSRSSSSSSSSTSGSSSRDSSSSTSSSSE
  SRSRSRGRGHNRDRKHRRSVDRKRRDTSGLERSHKSSKGGSSRDTKGSKDKNSRSDRKRSISESSRSGKR
  SSRSERDRKSDRKDKRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002687
• ORF Size: 2151 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002687.2, NM_002687.3, NP_002678.2
• RefSeq Size: 3606 bp
• RefSeq ORF: 2154 bp
• Locus ID: 5411
• Cytogenetics: 14q21.1
• Domains: pinin_SDK_memA, pinin_SDK_N
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• MW: 81.6 kDa
• Gene Summary: ''