RGS19 (NM_005873) Human Tagged ORF Clone

CAT#: RC209082

RGS19 (Myc-DDK-tagged)-Human regulator of G-protein signaling 19 (RGS19), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417015) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209082 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RGS19 protein (Cat# TP309082). The protein was produced from HEK293T cells transfected with RGS19 cDNA clone (Cat# RC209082) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RGS19
• Synonyms: GAIP; RGSGAIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209082 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCACCCCGCATGAGGCTGAGAAGCAGATCACAGGGCCAGAGGAGGCGGACCGGCCCCCTTCAATGT
  CCAGTCATGATACAGCCTCTCCAGCGGCCCCCAGCCGCAACCCCTGCTGCCTGTGCTGGTGCTGCTGCTG
  TAGCTGCTCCTGGAACCAAGAGCGGCGGCGCGCGTGGCAGGCCTCCCGGGAGAGCAAGCTGCAGCCCCTC
  CCCAGCTGTGAAGTATGTGCCACGCCAAGTCCTGAGGAGGTGCAGAGCTGGGCGCAGTCTTTTGACAAGC
  TGATGCACAGCCCAGCGGGACGCAGCGTGTTCCGGGCGTTCCTGCGGACAGAGTACAGCGAGGAGAACAT
  GCTCTTCTGGTTGGCCTGCGAGGAGCTGAAGGCCGAGGCCAACCAGCATGTGGTAGACGAGAAGGCGAGG
  CTCATCTACGAGGACTACGTATCCATCCTGTCCCCCAAGGAGGTGAGCCTGGACTCCCGTGTGCGGGAGG
  GCATCAACAAGAAGATGCAGGAGCCGTCCGCACACACGTTCGACGACGCGCAGCTGCAGATCTACACGCT
  CATGCACCGGGACTCCTACCCCCGCTTCCTCAGCTCTCCCACCTACCGTGCCCTGCTGCTGCAGGGGCCA
  TCACAGTCCTCCTCCGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209082 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPTPHEAEKQITGPEEADRPPSMSSHDTASPAAPSRNPCCLCWCCCCSCSWNQERRRAWQASRESKLQPL
  PSCEVCATPSPEEVQSWAQSFDKLMHSPAGRSVFRAFLRTEYSEENMLFWLACEELKAEANQHVVDEKAR
  LIYEDYVSILSPKEVSLDSRVREGINKKMQEPSAHTFDDAQLQIYTLMHRDSYPRFLSSPTYRALLLQGP
  SQSSSEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005873
• ORF Size: 651 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005873.1, NM_005873.2, NP_005864.1
• RefSeq Size: 1593 bp
• RefSeq ORF: 654 bp
• Locus ID: 10287
• Domains: RGS
• Protein Families: Druggable Genome
• MW: 24.6 kDa
• Gene Summary: G proteins mediate a number of cellular processes. The protein encoded by this gene belongs to the RGS (regulators of G-protein signaling) family and specifically interacts with G protein, GAI3. This protein is a guanosine triphosphatase-activating protein that functions to down-regulate Galpha i/Galpha q-linked signaling. Alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]