BCAR1 (NM_014567) Human Tagged ORF Clone

CAT#: RC209133

BCAR1 (Myc-DDK-tagged)-Human breast cancer anti-estrogen resistance 1 (BCAR1), transcript variant 6

Product Images

Western blot validation of overexpression lysate (Cat# LY415204) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209133 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BCAR1 protein (Cat# TP309133). The protein was produced from HEK293T cells transfected with BCAR1 cDNA clone (Cat# RC209133) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BCAR1
• Synonyms: CAS; CAS1; CASS1; CRKAS; P130Cas
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209133 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCACCTGAACGTGCTGGCCAAAGCGCTCTATGACAATGTGGCCGAGTCCCCGGATGAGCTCTCCT
  TCCGCAAGGGTGACATCATGACGGTGCTGGAGCAGGACACGCAGGGCCTGGACGGCTGGTGGCTCTGCTC
  GCTGCATGGGCGCCAGGGCATCGTGCCTGGGAACCGCCTCAAGATCTTGGTGGGCATGTATGATAAGAAG
  CCAGCAGGGCCTGGCTCCGGCCCTCCCGCCACCCCGGCCCAGCCTCAGCCTGGCCTCCATGCCCCAGCGC
  CTCCGGCCTCCCAGTACACGCCCATGCTCCCCAACACCTACCAGCCCCAGCCAGACAGCGTCTACCTGGT
  GCCCACTCCCAGCAAGGCTCAGCAAGGCCTCTACCAAGTCCCGGGTCCCAGCCCTCAGTTCCAGTCTCCC
  CCAGCCAAGCAGACATCCACCTTCTCGAAGCAGACACCCCATCACCCGTTTCCCAACCCGGCCACAGACC
  TGTACCAGGTGCCCCCAGGGCCTGGAGGCCCTGCCCAGGATATTTACCAGGTGCCACCTTCTGCCGGGAT
  GGGGCATGACATCTACCAGGTCCCCCCGTCCATGGACACACGCAGCTGGGAGGGCACGAAGCCCCCGGCA
  AAGGTGGTGGTGCCCACCCGCGTGGGGCAGGGCTATGTATACGAGGCCGCCCAGCCGGAGCAGGACGAGT
  ACGACATCCCGCGACACCTGCTGGCCCCGGGGCCACAGGACATCTATGATGTGCCCCCGGTTCGGGGGCT
  GCTTCCCAGCCAGTATGGCCAGGAGGTGTATGACACACCCCCCATGGCTGTCAAGGGTCCCAATGGCCGA
  GACCCGTTGCTGGAGGTGTATGACGTGCCCCCCAGTGTGGAGAAGGGCCTGCCACCGTCCAACCACCACG
  CAGTCTACGACGTTCCTCCATCGGTGAGCAAGGATGTGCCCGATGGCCCACTGCTGCGTGAGGAGACCTA
  CGATGTGCCCCCCGCCTTCGCCAAGGCCAAGCCCTTTGACCCGGCCCGCACCCCACTGGTACTGGCTGCG
  CCCCCTCCAGACTCCCCGCCGGCCGAGGACGTGTATGACGTGCCGCCCCCGGCTCCTGACCTCTACGACG
  TGCCCCCTGGCTTGCGGCGGCCTGGCCCGGGCACCCTGTACGATGTGCCCCGTGAACGGGTGCTTCCTCC
  TGAGGTGGCTGATGGTGGCGTGGTCGACAGTGGTGTGTATGCGGTGCCTCCCCCAGCTGAACGTGAAGCC
  CCGGCAGAGGGCAAGCGCCTGTCGGCCTCCAGCACCGGCAGCACACGCAGCAGCCAGTCTGCGTCCTCCT
  TGGAGGTGGCAGGGCCGGGCCGGGAACCCCTGGAGCTGGAAGTTGCTGTGGAGGCCCTGGCACGGCTGCA
  GCAGGGTGTGAGCGCCACCGTTGCCCACCTTCTGGACCTGGCAGGCAGCGCCGGTGCGACTGGGAGCTGG
  CGTAGCCCCTCTGAGCCACAGGAGCCGCTGGTGCAGGACCTGCAGGCTGCTGTGGCCGCCGTCCAGAGTG
  CCGTCCACGAGCTGTTGGAGTTTGCCCGCAGCGCGGTGGGCAATGCTGCCCACACATCTGACCGTGCCCT
  GCATGCCAAGCTTAGCCGGCAGCTGCAGAAGATGGAGGACGTGCACCAGACGCTGGTGGCACATGGTCAG
  GCCCTCGACGCTGGCCGGGGAGGCTCTGGAGCCACCCTTGAGGACCTGGACCGGCTGGTGGCCTGCTCGC
  GGGCTGTGCCCGAGGACGCCAAGCAGCTGGCCTCCTTCCTGCACGGCAATGCCTCACTGCTCTTCAGACG
  GACCAAGGCCACTGCCCCGGGGCCTGAGGGGGGTGGCACCCTGCACCCCAACCCCACTGACAAGACCAGC
  AGCATCCAGTCACGACCCCTGCCCTCACCCCCTAAGTTCACCTCCCAGGACTCGCCAGATGGGCAGTACG
  AGAACAGCGAGGGGGGCTGGATGGAGGACTATGACTACGTCCACCTACAGGGGAAGGAGGAGTTTGAGAA
  GACCCAGAAGGAGCTGCTGGAAAAGGGCAGCATCACGCGGCAGGGCAAGAGCCAGCTGGAGTTGCAGCAG
  CTGAAGCAGTTTGAACGACTGGAACAGGAGGTGTCACGGCCCATAGACCACGACCTGGCCAACTGGACGC
  CAGCCCAACCCCTGGCCCCGGGGCGAACAGGCGGCCTGGGGCCCTCGGACCGGCAGCTGCTGCTCTTCTA
  CCTGGAGCAGTGTGAGGCCAACCTGACCACACTGACCAACGCCGTGGACGCCTTCTTTACCGCCGTGGCC
  ACCAACCAGCCGCCCAAGATCTTTGTGGCGCACAGCAAGTTCGTCATCCTCAGCGCCCACAAGCTGGTGT
  TCATCGGGGACACACTGTCACGGCAGGCCAAGGCTGCTGACGTGCGCAGCCAGGTGACCCACTACAGCAA
  CCTGCTGTGCGACCTCCTGCGCGGCATCGTGGCCACCACCAAGGCCGCTGCCTTGCAGTACCCATCGCCT
  TCCGCGGCCCAGGACATGGTGGAGAGGGTCAAGGAGCTGGGCCACAGCACCCAGCAGTTCCGCCGCGTCC
  TAGGCCAGCTGGCAGCCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209133 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKK
  PAGPGSGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSP
  PAKQTSTFSKQTPHHPFPNPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPA
  KVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGR
  DPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAA
  PPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREA
  PAEGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSW
  RSPSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQ
  ALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTS
  SIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQ
  LKQFERLEQEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVA
  TNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSP
  SAAQDMVERVKELGHSTQQFRRVLGQLAAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014567
• ORF Size: 2610 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014567.1, NM_014567.2, NM_014567.3, NP_055382.2
• RefSeq Size: 3257 bp
• RefSeq ORF: 2613 bp
• Locus ID: 9564
• Domains: SH3, Extensin_2
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• MW: 93.4 kDa
• Gene Summary: The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017]