FKLF / KLF11 (KLF11) (NM_003597) Human Tagged ORF Clone

CAT#: RC209194

KLF11 (Myc-DDK-tagged)-Human Kruppel-like factor 11 (KLF11), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418566) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209194 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLF11
• Synonyms: FKLF; FKLF1; MODY7; TIEG2; Tieg3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209194 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACACGCCGGACTTCGCAGGCCCAGACGACGCGCGCGCAGTTGACATCATGGACATATGTGAGTCCA
  TCCTGGAGAGGAAGCGGCATGACAGCGAAAGGTCTACTTGCAGCATCTTGGAGCAGACAGACATGGAAGC
  TGTCGAGGCTCTTGTTTGTATGAGCTCCTGGGGTCAAAGATCCCAGAAAGGTGACCTGTTGCGGATAAGA
  CCCCTCACGCCTGTCTCTGACTCTGGGGATGTCACCACCACTGTGCATATGGATGCAGCCACACCTGAAC
  TACCAAAAGACTTCCATTCTTTATCGACTCTGTGCATAACTCCTCCTCAGAGCCCTGATCTCGTGGAGCC
  ATCGACAAGGACACCTGTTTCTCCCCAAGTAACAGATTCCAAAGCATGTACAGCCACGGATGTTCTCCAG
  TCCTCTGCCGTAGTGGCCAGAGCTCTGAGCGGGGGCGCGGAGAGGGGCTTGCTGGGTTTGGAGCCAGTGC
  CCAGCTCTCCCTGCAGGGCCAAGGGGACTAGCGTGATCCGACACACTGGGGAGAGCCCTGCTGCCTGCTT
  TCCCACCATCCAGACTCCAGATTGCCGGCTTTCTGACAGCAGAGAAGGAGAAGAGCAGCTTCTGGGACAC
  TTTGAAACTTTGCAGGACACACACCTCACGGACAGTTTACTCAGCACTAACTTGGTGTCCTGTCAGCCCT
  GCTTGCACAAGTCTGGTGGCCTGCTGCTCACTGACAAAGGCCAGCAGGCAGGGTGGCCTGGTGCAGTTCA
  GACTTGCTCACCAAAGAATTATGAAAATGACCTGCCCAGGAAAACCACCCCTCTGATTTCTGTCTCTGTC
  CCTGCTCCCCCTGTCCTTTGCCAGATGATCCCTGTGACTGGACAAAGTAGCATGTTACCAGCTTTTTTGA
  AGCCCCCTCCCCAGTTGTCTGTGGGGACTGTGAGACCCATCCTAGCTCAGGCTGCTCCAGCGCCTCAACC
  TGTGTTCGTGGGACCTGCTGTGCCTCAGGGAGCTGTGATGTTGGTCCTGCCCCAGGGAGCCCTCCCTCCG
  CCTGCCCCCTGTGCAGCCAATGTCATGGCTGCCGGGAATACCAAGTTGTTGCCCCTTGCCCCTGCTCCAG
  TGTTCATCACCTCTAGCCAAAACTGTGTCCCTCAGGTAGACTTTTCCCGAAGGAGGAACTATGTTTGCAG
  CTTCCCAGGTTGCCGGAAGACCTACTTCAAAAGTTCCCACCTTAAGGCCCATCTTCGCACTCACACAGGG
  GAGAAGCCTTTCAACTGCAGCTGGGATGGCTGTGATAAAAAGTTTGCTCGTTCGGATGAGCTGTCACGCC
  ACCGCAGAACTCACACAGGGGAGAAGAAGTTTGTGTGCCCGGTGTGTGACCGACGTTTCATGCGCAGTGA
  CCACCTGACGAAGCATGCCCGGCGCCACATGACGACCAAGAAGATCCCAGGCTGGCAGGCAGAGGTTGGC
  AAGCTGAACAGAATCGCCTCTGCAGAGAGCCCGGGGAGCCCACTGGTGAGCATGCCAGCCTCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209194 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHTPDFAGPDDARAVDIMDICESILERKRHDSERSTCSILEQTDMEAVEALVCMSSWGQRSQKGDLLRIR
  PLTPVSDSGDVTTTVHMDAATPELPKDFHSLSTLCITPPQSPDLVEPSTRTPVSPQVTDSKACTATDVLQ
  SSAVVARALSGGAERGLLGLEPVPSSPCRAKGTSVIRHTGESPAACFPTIQTPDCRLSDSREGEEQLLGH
  FETLQDTHLTDSLLSTNLVSCQPCLHKSGGLLLTDKGQQAGWPGAVQTCSPKNYENDLPRKTTPLISVSV
  PAPPVLCQMIPVTGQSSMLPAFLKPPPQLSVGTVRPILAQAAPAPQPVFVGPAVPQGAVMLVLPQGALPP
  PAPCAANVMAAGNTKLLPLAPAPVFITSSQNCVPQVDFSRRRNYVCSFPGCRKTYFKSSHLKAHLRTHTG
  EKPFNCSWDGCDKKFARSDELSRHRRTHTGEKKFVCPVCDRRFMRSDHLTKHARRHMTTKKIPGWQAEVG
  KLNRIASAESPGSPLVSMPASA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003597
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003597.1, NM_003597.2, NM_003597.3, NM_003597.4, NP_003588.1
• RefSeq Size: 4051 bp
• RefSeq ORF: 1539 bp
• Locus ID: 8462
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• MW: 55.1 kDa
• Gene Summary: The protein encoded by this gene is a zinc finger transcription factor that binds to SP1-like sequences in epsilon- and gamma-globin gene promoters. This binding inhibits cell growth and causes apoptosis. Defects in this gene are a cause of maturity-onset diabetes of the young type 7 (MODY7). Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]