SCARA3 (NM_182826) Human Tagged ORF Clone

CAT#: RC209235

SCARA3 (Myc-DDK-tagged)-Human scavenger receptor class A, member 3 (SCARA3), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405369) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209235 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCARA3
• Synonyms: APC7; CSR; CSR1; MSLR1; MSRL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209235 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTGAGGTCGGCCGGCGGCGATGGAGATGCCTTGTGCGTTACAGAAGAGGACCTGGCGGGTGACG
  ACGAGGACATGCCGACCTTCCCATGCACCCAGAAGGGCCGGCCAGGGCCCCGCTGCAGCCGCTGCCAGAA
  GAACCTATCTTTGCACACATCGGTGCGGATTCTTTACCTCTTCCTGGCCCTGCTCCTGGTGGCCGTGGCT
  GTGTTGGCCTCTCTGGTTTTCAGAAAAGTGGACTCTCTCTCCGAAGACATCTCCTTGACCCAGTCTATTT
  ATGACAAGAAGCTTGTGTTAATGCAGAAAAATCTCCAGGGCCTGGATCCGAAAGCCCTGAACAACTGCTC
  TTTCTGCCATGAAGCTGGGCAGCTGGGGCCAGAGATCCGAAAACTGCAGGAGGAGCTGGAGGGAATTCAG
  AAGCTGCTTCTGGCCCAGGAGGTGCAGCTGGACCAGACCTTACAGGCCCAGGAGGTGCTCTCCACCACCA
  GCAGACAAATCTCCCAGGAGATGGGCAGTTGCTCCTTCTCCATCCACCAGGTTAACCAGTCTCTGGGGCT
  CTTCCTGGCCCAGGTGAGAGGCTGGCAGGCCACCACAGCTGGCCTGGACCTCTCTCTGAAGGACCTCACC
  CAGGAGTGCTACGATGTCAAGGCTGCAGTGCACCAGATCAACTTCACCGTGGGGCAGACTTCCGAGTGGA
  TCCACGGGATCCAGCGGAAGACAGACGAGGAGACCCTGACCCTCCAGAAGATTGTCACCGACTGGCAGAA
  CTACACACGGCTCTTCAGCGGCCTGCGCACCACCTCCACCAAGACTGGAGAGGCGGTCAAGAACATCCAG
  GCCACCCTGGGGGCCTCCTCACAGCGCATCAGCCAGAACTCAGAGAGCATGCACGACCTGGTACTCCAGG
  TCATGGGCTTGCAGCTGCAGCTGGATAACATCTCGTCCTTCCTGGATGACCACGAAGAGAACATGCATGA
  TCTTCAGTACCATACCCACTACGCCCAGAACCGCACTGTGGAGAGGTTTGAGTCTCTGGAAGGACGCATG
  GCTTCTCACGAGATTGAAATTGGCACCATCTTCACCAACATCAATGCCACCGACAACCACGTGCACAGCA
  TGCTCAAGTACCTGGATGACGTGCGGCTCTCCTGCACGCTGGGCTTCCACACCCATGCCGAGGAGCTCTA
  CTACCTGAACAAGTCTGTCTCCATCATGCTGGGCACCACAGACCTGCTCCGGGAGCGCTTCAGCCTGCTC
  AGTGCCCGGCTGGACCTCAACGTCCGGAACCTCTCCATGATCGTGGAGGAGATGAAGGCAGTGGACACAC
  AGCATGGAGAAATCCTTCGCAATGTCACCATCCTACGAGGTCACACTTTGTTTTCACATAATCGGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209235 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKVRSAGGDGDALCVTEEDLAGDDEDMPTFPCTQKGRPGPRCSRCQKNLSLHTSVRILYLFLALLLVAVA
  VLASLVFRKVDSLSEDISLTQSIYDKKLVLMQKNLQGLDPKALNNCSFCHEAGQLGPEIRKLQEELEGIQ
  KLLLAQEVQLDQTLQAQEVLSTTSRQISQEMGSCSFSIHQVNQSLGLFLAQVRGWQATTAGLDLSLKDLT
  QECYDVKAAVHQINFTVGQTSEWIHGIQRKTDEETLTLQKIVTDWQNYTRLFSGLRTTSTKTGEAVKNIQ
  ATLGASSQRISQNSESMHDLVLQVMGLQLQLDNISSFLDDHEENMHDLQYHTHYAQNRTVERFESLEGRM
  ASHEIEIGTIFTNINATDNHVHSMLKYLDDVRLSCTLGFHTHAEELYYLNKSVSIMLGTTDLLRERFSLL
  SARLDLNVRNLSMIVEEMKAVDTQHGEILRNVTILRGHTLFSHNRI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182826
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182826.1, NP_878185.1
• RefSeq Size: 1711 bp
• RefSeq ORF: 1401 bp
• Locus ID: 51435
• Protein Families: Druggable Genome, Transmembrane
• MW: 52.4 kDa
• Gene Summary: This gene encodes a macrophage scavenger receptor-like protein. This protein has been shown to deplete reactive oxygen species, and thus play an important role in protecting cells from oxidative stress. The expression of this gene is induced by oxidative stress. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]