HS3ST3B1 (NM_006041) Human Tagged ORF Clone

CAT#: RC209249L2

Lenti ORF clone of Human heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 (HS3ST3B1), mGFP tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: HS3ST3B1
• Synonyms: 3-OST-3B; 3OST3B1; h3-OST-3B
• Vector: pLenti-C-mGFP
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC209249).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_006041
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006041.1, NP_006032.1
• RefSeq Size: 2032
• RefSeq ORF: 1173
• Locus ID: 9953
• Domains: Sulfotransfer
• Protein Families: Transmembrane
• Protein Pathways: Glycosaminoglycan degradation, Heparan sulfate biosynthesis
• MW: 43.1 kDa
• Gene Summary: The protein encoded by this gene is a type II integral membrane protein that belongs to the 3-O-sulfotransferases family. These proteins catalyze the addition of sulfate groups at the 3-OH position of glucosamine in heparan sulfate. The substrate specificity of individual members of the family is based on prior modification of the heparan sulfate chain, thus allowing different members of the family to generate binding sites for different proteins on the same heparan sulfate chain. Following treatment with a histone deacetylase inhibitor, expression of this gene is activated in a pancreatic cell line. The increased expression results in promotion of the epithelial-mesenchymal transition. In addition, the modification catalyzed by this protein allows herpes simplex virus membrane fusion and penetration. A very closely related homolog with an almost identical sulfotransferase domain maps less than 1 Mb away. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]