epithelial Sodium Channel gamma (SCNN1G) (NM_001039) Human Tagged ORF Clone

CAT#: RC209264

SCNN1G (Myc-DDK-tagged)-Human sodium channel, nonvoltage-gated 1, gamma (SCNN1G)

Product Images

Western blot validation of overexpression lysate (Cat# LY422011) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209264 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCNN1G
• Synonyms: BESC3; ENaCg; ENaCgamma; LDLS2; PHA1; SCNEG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209264 representing NM_001039
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCCGGAGAGAAGATCAAAGCCAAAATCAAGAAGAATCTGCCCGTGACGGGCCCTCAGGCGCCGA
  CCATTAAAGAGCTGATGCGGTGGTACTGCCTCAACACCAACACCCATGGCTGTCGCCGCATCGTGGTGTC
  CCGCGGCCGTCTGCGCCGCCTCCTCTGGATCGGGTTCACACTGACTGCCGTGGCCCTCATCCTCTGGCAG
  TGCGCCCTCCTCGTCTTCTCCTTCTATACTGTCTCAGTTTCCATCAAAGTCCACTTCCGGAAGCTGGATT
  TTCCTGCAGTCACCATCTGCAACATCAACCCCTACAAGTACAGCACCGTTCGCCACCTTCTAGCTGACTT
  GGAACAGGAGACCAGAGAGGCCCTGAAGTCCCTGTATGGCTTTCCAGAGTCCCGGAAGCGCCGAGAGGCG
  GAGTCCTGGAACTCCGTCTCAGAGGGAAAGCAGCCTAGATTCTCCCACCGGATTCCGCTGCTGATCTTTG
  ATCAGGATGAGAAGGGCAAGGCCAGGGACTTCTTCACAGGGAGGAAGCGGAAAGTCGGCGGTAGCATCAT
  TCACAAGGCTTCAAATGTCATGCACATCGAGTCCAAGCAAGTGGTGGGATTCCAACTGTGCTCAAATGAC
  ACCTCCGACTGTGCCACCTACACCTTCAGCTCGGGAATCAATGCCATTCAGGAGTGGTATAAGCTACACT
  ACATGAACATCATGGCACAGGTGCCTCTGGAGAAGAAAATCAACATGAGCTATTCTGCTGAGGAGCTGCT
  GGTGACCTGCTTCTTTGATGGAGTGTCCTGTGATGCCAGGAATTTCACGCTTTTCCACCACCCGATGCAT
  GGGAATTGCTATACTTTCAACAACAGAGAAAATGAGACCATTCTCAGCACCTCCATGGGGGGCAGCGAAT
  ATGGGCTGCAAGTCATTTTGTACATAAACGAAGAGGAATACAACCCATTCCTCGTGTCCTCCACTGGAGC
  TAAGGTGATCATCCATCGGCAGGATGAGTATCCCTTCGTCGAAGATGTGGGAACAGAGATTGAGACAGCA
  ATGGTCACCTCTATAGGAATGCACCTGACAGAGTCCTTCAAGCTGAGTGAGCCCTACAGTCAGTGCACGG
  AGGACGGGAGTGACGTGCCAATCAGGAACATCTACAACGCTGCCTACTCGCTCCAGATCTGCCTTCATTC
  ATGCTTCCAGACAAAGATGGTGGAGAAATGTGGGTGTGCCCAGTACAGCCAGCCTCTACCTCCTGCAGCC
  AACTACTGCAACTACCAGCAGCACCCCAACTGGATGTATTGTTACTACCAACTGCATCGAGCCTTTGTCC
  AGGAAGAGCTGGGCTGCCAGTCTGTGTGCAAGGAAGCCTGCAGCTTTAAAGAGTGGACACTAACCACAAG
  CCTGGCACAATGGCCATCTGTGGTTTCGGAGAAGTGGTTGCTGCCTGTTCTCACTTGGGACCAAGGCCGG
  CAAGTAAACAAAAAGCTCAACAAGACAGACTTGGCCAAACTCTTGATATTCTACAAAGACCTGAACCAGA
  GATCCATCATGGAGAGCCCAGCCAACAGTATTGAGATGCTTCTGTCCAACTTCGGTGGCCAGCTGGGCCT
  GTGGATGAGCTGCTCTGTTGTCTGCGTCATCGAGATCATCGAGGTCTTCTTCATTGACTTCTTCTCTATC
  ATTGCCCGCCGCCAGTGGCAGAAAGCCAAGGAGTGGTGGGCCTGGAAACAGGCTCCCCCATGTCCAGAAG
  CTCCCCGTAGCCCACAGGGCCAGGACAATCCAGCCCTGGATATAGACGATGACCTACCCACTTTCAACTC
  TGCTTTGCACCTGCCTCCAGCCCTAGGAACCCAAGTGCCCGGCACACCGCCCCCCAAATACAATACCTTG
  CGCTTGGAGAGGGCCTTTTCCAACCAGCTCACAGATACCCAGATGCTGGATGAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209264 representing NM_001039
  Red=Cloning site Green=Tags(s)
  
  MAPGEKIKAKIKKNLPVTGPQAPTIKELMRWYCLNTNTHGCRRIVVSRGRLRRLLWIGFTLTAVALILWQ
  CALLVFSFYTVSVSIKVHFRKLDFPAVTICNINPYKYSTVRHLLADLEQETREALKSLYGFPESRKRREA
  ESWNSVSEGKQPRFSHRIPLLIFDQDEKGKARDFFTGRKRKVGGSIIHKASNVMHIESKQVVGFQLCSND
  TSDCATYTFSSGINAIQEWYKLHYMNIMAQVPLEKKINMSYSAEELLVTCFFDGVSCDARNFTLFHHPMH
  GNCYTFNNRENETILSTSMGGSEYGLQVILYINEEEYNPFLVSSTGAKVIIHRQDEYPFVEDVGTEIETA
  MVTSIGMHLTESFKLSEPYSQCTEDGSDVPIRNIYNAAYSLQICLHSCFQTKMVEKCGCAQYSQPLPPAA
  NYCNYQQHPNWMYCYYQLHRAFVQEELGCQSVCKEACSFKEWTLTTSLAQWPSVVSEKWLLPVLTWDQGR
  QVNKKLNKTDLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFFIDFFSI
  IARRQWQKAKEWWAWKQAPPCPEAPRSPQGQDNPALDIDDDLPTFNSALHLPPALGTQVPGTPPPKYNTL
  RLERAFSNQLTDTQMLDEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039
• ORF Size: 1947 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039.1, NM_001039.2, NM_001039.3, NP_001030.2
• RefSeq Size: 3516 bp
• RefSeq ORF: 1950 bp
• Locus ID: 6340
• Cytogenetics: 16p12.2
• Domains: ASC
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Protein Pathways: Taste transduction
• MW: 74.1 kDa
• Gene Summary: 'Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome. [provided by RefSeq, Apr 2009]'