DUSP9 (NM_001395) Human Tagged ORF Clone
CAT#: RC209271
DUSP9 (Myc-DDK-tagged)-Human dual specificity phosphatase 9 (DUSP9)
"NM_001395" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | DUSP9 |
Synonyms | MKP-4; MKP4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209271 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGTCTGGGCCGCTCGTGCCTGTGGCTGCGTCGGGAGCTGTCGCCCCCGCGGCCGCGGCTCCTGC TCCTGGACTGCCGCAGCCGCGAGCTGTACGAGTCGGCGCGCATCGGTGGGGCGCTGAGCGTGGCCCTGCC GGCGCTCCTGCTGCGCCGCCTGCGGAGGGGCAGCCTGTCGGTGCGCGCGCTCCTGCCTGGGCCGCCGCTG CAGCCGCCCCCGCCTGCCCCCGTGCTCCTGTACGACCAGGGCGGGGGCCGGCGCCGGCGCGGGGAGGCCG AGGCCGAGGCCGAGGAGTGGGAGGCCGAGTCGGTGCTGGGCACCCTGCTGCAGAAGCTGCGAGAGGAAGG CTACCTGGCCTACTACCTCCAGGGAGGCTTCAGCAGATTCCAGGCCGAGTGCCCTCACCTGTGTGAGACC AGCCTTGCTGGCCGTGCCGGCTCCAGCATGGCGCCGTTGCCCGGTCCAGTGCCCGTGGTGGGGTTGGGCA GCCTGTGCCTGGGCTCCGACTGCTCTGATGCGGAATCCGAGGCTGACCGCGACTCCATGAGCTGTGGCCT GGATTCGGAGGGTGCCACACCCCCACCAGTGGGGCTGCGGGCATCCTTCCCTGTCCAGATCCTGCCCAAC CTCTATCTGGGCAGTGCCCGGGATTCCGCCAATTTGGAGAGCCTGGCCAAACTGGGCATCCGCTACATCC TCAATGTCACCCCCAACCTCCCAAACTTCTTCGAGAAGAATGGTGACTTTCACTACAAGCAGATCCCCAT CTCCGACCACTGGAGCCAGAACCTGTCGCGGTTCTTTCCGGAGGCCATTGAGTTCATTGATGAGGCCTTG TCCCAGAACCGCGGGGTGCTCGTCCACTGCTTGGCGGGGGTCAGCCGTTCTGTCACCGTCACTGTGGCCT ACCTCATGCAGAAGCTCCACCTCTCTCTCAACGATGCCTATGACCTGGTCAAGAGGAAGAAGTCTAACAT CTCCCCCAACTTCAACTTCATGGGGCAGTTGCTGGACTTTGAGCGCAGCTTGCGGCTGGAGGAGCGCCAC TCGCAGGAGCAGGGCAGTGGGGGGCAGGCATCTGCGGCCTCCAACCCGCCCTCCTTCTTCACCACCCCCA CCAGTGATGGCGCCTTCGAGCTGGCCCCCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209271 protein sequence
Red=Cloning site Green=Tags(s) MEGLGRSCLWLRRELSPPRPRLLLLDCRSRELYESARIGGALSVALPALLLRRLRRGSLSVRALLPGPPL QPPPPAPVLLYDQGGGRRRRGEAEAEAEEWEAESVLGTLLQKLREEGYLAYYLQGGFSRFQAECPHLCET SLAGRAGSSMAPLPGPVPVVGLGSLCLGSDCSDAESEADRDSMSCGLDSEGATPPPVGLRASFPVQILPN LYLGSARDSANLESLAKLGIRYILNVTPNLPNFFEKNGDFHYKQIPISDHWSQNLSRFFPEAIEFIDEAL SQNRGVLVHCLAGVSRSVTVTVAYLMQKLHLSLNDAYDLVKRKKSNISPNFNFMGQLLDFERSLRLEERH SQEQGSGGQASAASNPPSFFTTPTSDGAFELAPT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001395 |
ORF Size | 1152 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001395.1, NM_001395.2, NM_001395.3, NP_001386.1 |
RefSeq Size | 2394 bp |
RefSeq ORF | 1155 bp |
Locus ID | 1852 |
Cytogenetics | Xq28 |
Domains | DSPc, RHOD |
Protein Families | Phosphatase |
Protein Pathways | MAPK signaling pathway |
MW | 41.9 kDa |
Gene Summary | 'The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product shows selectivity for members of the ERK family of MAP kinases and is localized to the cytoplasm and nucleus. Aberrant expression of this gene is associated with type 2 diabetes and cancer progression in several cell types. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119259 | DUSP9 (untagged)-Human dual specificity phosphatase 9 (DUSP9) |
USD 540.00 |
|
RG209271 | DUSP9 (GFP-tagged) - Human dual specificity phosphatase 9 (DUSP9) |
USD 460.00 |
|
RC209271L3 | Lenti ORF clone of Human dual specificity phosphatase 9 (DUSP9), Myc-DDK-tagged |
USD 620.00 |
|
RC209271L4 | Lenti ORF clone of Human dual specificity phosphatase 9 (DUSP9), mGFP tagged |
USD 620.00 |
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