DUSP9 (NM_001395) Human Tagged ORF Clone

CAT#: RG209271

DUSP9 (GFP-tagged) - Human dual specificity phosphatase 9 (DUSP9)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DUSP9
• Synonyms: MKP-4; MKP4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209271 representing NM_001395
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGTCTGGGCCGCTCGTGCCTGTGGCTGCGTCGGGAGCTGTCGCCCCCGCGGCCGCGGCTCCTGC
  TCCTGGACTGCCGCAGCCGCGAGCTGTACGAGTCGGCGCGCATCGGTGGGGCGCTGAGCGTGGCCCTGCC
  GGCGCTCCTGCTGCGCCGCCTGCGGAGGGGCAGCCTGTCGGTGCGCGCGCTCCTGCCTGGGCCGCCGCTG
  CAGCCGCCCCCGCCTGCCCCCGTGCTCCTGTACGACCAGGGCGGGGGCCGGCGCCGGCGCGGGGAGGCCG
  AGGCCGAGGCCGAGGAGTGGGAGGCCGAGTCGGTGCTGGGCACCCTGCTGCAGAAGCTGCGAGAGGAAGG
  CTACCTGGCCTACTACCTCCAGGGAGGCTTCAGCAGATTCCAGGCCGAGTGCCCTCACCTGTGTGAGACC
  AGCCTTGCTGGCCGTGCCGGCTCCAGCATGGCGCCGTTGCCCGGTCCAGTGCCCGTGGTGGGGTTGGGCA
  GCCTGTGCCTGGGCTCCGACTGCTCTGATGCGGAATCCGAGGCTGACCGCGACTCCATGAGCTGTGGCCT
  GGATTCGGAGGGTGCCACACCCCCACCAGTGGGGCTGCGGGCATCCTTCCCTGTCCAGATCCTGCCCAAC
  CTCTATCTGGGCAGTGCCCGGGATTCCGCCAATTTGGAGAGCCTGGCCAAACTGGGCATCCGCTACATCC
  TCAATGTCACCCCCAACCTCCCAAACTTCTTCGAGAAGAATGGTGACTTTCACTACAAGCAGATCCCCAT
  CTCCGACCACTGGAGCCAGAACCTGTCGCGGTTCTTTCCGGAGGCCATTGAGTTCATTGATGAGGCCTTG
  TCCCAGAACCGCGGGGTGCTCGTCCACTGCTTGGCGGGGGTCAGCCGTTCTGTCACCGTCACTGTGGCCT
  ACCTCATGCAGAAGCTCCACCTCTCTCTCAACGATGCCTATGACCTGGTCAAGAGGAAGAAGTCTAACAT
  CTCCCCCAACTTCAACTTCATGGGGCAGTTGCTGGACTTTGAGCGCAGCTTGCGGCTGGAGGAGCGCCAC
  TCGCAGGAGCAGGGCAGTGGGGGGCAGGCATCTGCGGCCTCCAACCCGCCCTCCTTCTTCACCACCCCCA
  CCAGTGATGGCGCCTTCGAGCTGGCCCCCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209271 representing NM_001395
  Red=Cloning site Green=Tags(s)
  
  MEGLGRSCLWLRRELSPPRPRLLLLDCRSRELYESARIGGALSVALPALLLRRLRRGSLSVRALLPGPPL
  QPPPPAPVLLYDQGGGRRRRGEAEAEAEEWEAESVLGTLLQKLREEGYLAYYLQGGFSRFQAECPHLCET
  SLAGRAGSSMAPLPGPVPVVGLGSLCLGSDCSDAESEADRDSMSCGLDSEGATPPPVGLRASFPVQILPN
  LYLGSARDSANLESLAKLGIRYILNVTPNLPNFFEKNGDFHYKQIPISDHWSQNLSRFFPEAIEFIDEAL
  SQNRGVLVHCLAGVSRSVTVTVAYLMQKLHLSLNDAYDLVKRKKSNISPNFNFMGQLLDFERSLRLEERH
  SQEQGSGGQASAASNPPSFFTTPTSDGAFELAPT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001395
• ORF Size: 1152 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001395.1, NP_001386.1
• RefSeq Size: 2303 bp
• RefSeq ORF: 1155 bp
• Locus ID: 1852
• Cytogenetics: Xq28
• Domains: DSPc, RHOD
• Protein Families: Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product shows selectivity for members of the ERK family of MAP kinases and is localized to the cytoplasm and nucleus. Aberrant expression of this gene is associated with type 2 diabetes and cancer progression in several cell types. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]'