IPMK (NM_152230) Human Tagged ORF Clone

CAT#: RC209343

IPMK (Myc-DDK-tagged)-Human inositol polyphosphate multikinase (IPMK)

Product Images

Western blot validation of overexpression lysate (Cat# LY407707) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209343 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IPMK protein (Cat# TP309343). The protein was produced from HEK293T cells transfected with IPMK cDNA clone (Cat# RC209343) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IPMK
• Synonyms: Impk; inositol polyphosphate kinase 2; inositol polyphosphate multikinase; Ipk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209343 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACAGAGCCACCATCCCCCCTCCGGGTCGAGGCGCCGGGCCCCCCAGAAATGCGGACCTCACCGG
  CGATCGAGTCCACCCCTGAGGGCACCCCGCAGCCGGCGGGCGGCAGACTCCGCTTCCTCAACGGCTGCGT
  GCCCCTCTCGCATCAGGTGGCCGGGCACATGTACGGGAAGGACAAAGTGGGTATACTGCAACATCCAGAT
  GGCACAGTTTTGAAACAGTTACAACCACCTCCAAGGGGCCCAAGAGAGCTGGAATTCTATAATATGGTTT
  ATGCTGCTGACTGTTTTGATGGTGTTCTTCTAGAGCTACGAAAATATTTGCCAAAATATTATGGCATCTG
  GTCACCTCCCACTGCACCAAACGATTTATACCTAAAACTGGAAGATGTGACCCATAAATTTAATAAGCCC
  TGTATAATGGATGTAAAGATAGGGCAAAAAAGCTATGATCCTTTTGCCTCATCTGAGAAGATTCAGCAAC
  AGGTCAGCAAGTACCCATTAATGGAAGAGATTGGGTTCTTGGTGCTTGGCATGAGGGTTTATCATGTTCA
  TTCCGATAGCTATGAGACAGAAAACCAGCATTACGGAAGAAGCTTAACAAAAGAAACTATAAAGGATGGA
  GTCTCCAGATTTTTTCATAATGGGTACTGCTTAAGAAAAGATGCTGTTGCTGCCAGTATTCAGAAGATTG
  AGAAAATTCTGCAGTGGTTTGAAAACCAGAAGCAGCTTAATTTTTACGCAAGTTCATTACTCTTTGTTTA
  TGAAGGTTCATCTCAGCCAACCACTACAAAATTGAATGACAGAACTTTGGCAGAAAAGTTTTTGTCCAAA
  GGACAACTGTCAGACACAGAAGTACTAGAGTACAATAATAACTTTCATGTGTTAAGTTCCACAGCTAATG
  GAAAAATAGAGTCTTCAGTGGGCAAAAGCTTGTCCAAGATGTATGCGCGTCACAGGAAAATATATACAAA
  AAAGCATCACAGTCAGACTTCATTGAAAGTTGAAAATCTGGAGCAAGACAATGGGTGGAAAAGCATGTCA
  CAGGAACATTTAAATGGAAATGTACTTTCCCAACTGGAAAAAGTTTTCTACCATCTTCCCACTGGTTGCC
  AAGAGATTGCTGAAGTAGAAGTGCGAATGATAGATTTTGCTCATGTGTTCCCTAGCAACACAATAGATGA
  GGGATATGTTTATGGGCTAAAGCATTTAATTTCTGTACTTCGAAGTATTTTAGACAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209343 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATEPPSPLRVEAPGPPEMRTSPAIESTPEGTPQPAGGRLRFLNGCVPLSHQVAGHMYGKDKVGILQHPD
  GTVLKQLQPPPRGPRELEFYNMVYAADCFDGVLLELRKYLPKYYGIWSPPTAPNDLYLKLEDVTHKFNKP
  CIMDVKIGQKSYDPFASSEKIQQQVSKYPLMEEIGFLVLGMRVYHVHSDSYETENQHYGRSLTKETIKDG
  VSRFFHNGYCLRKDAVAASIQKIEKILQWFENQKQLNFYASSLLFVYEGSSQPTTTKLNDRTLAEKFLSK
  GQLSDTEVLEYNNNFHVLSSTANGKIESSVGKSLSKMYARHRKIYTKKHHSQTSLKVENLEQDNGWKSMS
  QEHLNGNVLSQLEKVFYHLPTGCQEIAEVEVRMIDFAHVFPSNTIDEGYVYGLKHLISVLRSILDN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152230
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152230.1, NM_152230.2, NM_152230.3, NM_152230.4, NP_689416.1
• RefSeq Size: 6133 bp
• RefSeq ORF: 1251 bp
• Locus ID: 253430
• Protein Pathways: Inositol phosphate metabolism
• MW: 47.2 kDa
• Gene Summary: This gene encodes a member of the inositol phosphokinase family. The encoded protein has 3-kinase, 5-kinase and 6-kinase activities on phosphorylated inositol substrates. The encoded protein plays an important role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate, and has a preferred 5-kinase activity. This gene may play a role in nuclear mRNA export. Pseudogenes of this gene are located on the long arm of chromosome 13 and the short arm of chromosome 19. [provided by RefSeq, Dec 2010]