Adducin 2 (ADD2) (NM_001617) Human Tagged ORF Clone

CAT#: RC209517

ADD2 (Myc-DDK-tagged)-Human adducin 2 (beta) (ADD2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY419845) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209517 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ADD2 protein (Cat# TP309517). The protein was produced from HEK293T cells transfected with ADD2 cDNA clone (Cat# RC209517) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADD2
• Synonyms: ADDB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209517 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAAGAGACGGTCCCCGAGGCTGCCTCGCCGCCGCCCCCGCAGGGGCAGCCTTACTTTGACCGCT
  TCTCAGAGGACGACCCCGAGTACATGCGCCTTCGCAACCGGGCGGCGGACCTGCGGCAGGACTTCAACCT
  GATGGAGCAGAAGAAGCGCGTCACCATGATCCTGCAGAGTCCCTCTTTCAGGGAGGAGCTGGAAGGCCTC
  ATCCAGGAGCAGATGAAGAAGGGGAACAACTCCTCCAACATCTGGGCCCTGCGACAGATCGCGGACTTCA
  TGGCCAGCACCTCCCACGCAGTCTTCCCGACATCTTCCATGAATGTCTCCATGATGACGCCTATCAATGA
  CCTCCACACAGCTGACTCCCTGAACCTGGCCAAAGGGGAGCGGCTCATGCGGTGCAAGATCAGCAGTGTC
  TACCGACTCCTGGACCTCTATGGCTGGGCCCAGCTGAGTGACACCTATGTCACGTTGAGAGTCAGCAAGG
  AGCAGGACCACTTCCTGATCAGCCCTAAGGGAGTTTCTTGCAGTGAAGTCACAGCGTCCAGCCTGATCAA
  GGTGAACATTCTGGGAGAGGTGGTGGAGAAGGGCAGCAGCTGCTTCCCAGTGGACACCACAGGCTTCTGT
  CTGCACTCGGCCATCTATGCAGCGAGGCCCGACGTGCGCTGCATCATCCACCTGCACACACCGGCCACAG
  CAGCGGTGTCGGCCATGAAGTGGGGCCTCCTGCCTGTCTCCCACAATGCCCTGCTGGTGGGGGACATGGC
  CTATTATGACTTCAATGGGGAAATGGAGCAGGAAGCCGATCGGATCAACCTGCAGAAGTGCCTTGGACCC
  ACCTGCAAGATCCTGGTGCTAAGAAACCATGGAGTGGTTGCTCTGGGTGACACGGTAGAGGAGGCATTTT
  ACAAGATCTTCCACCTGCAGGCTGCATGTGAGATACAGGTGTCGGCTCTGTCCAGTGCCGGGGGAGTGGA
  GAACCTCATCCTCCTGGAGCAGGAGAAGCACCGGCCCCATGAGGTGGGCTCCGTGCAGTGGGCCGGGAGC
  ACCTTTGGGCCTATGCAGAAGAGTCGGCTGGGGGAGCATGAGTTTGAGGCCCTCATGAGGATGCTGGACA
  ACCTGGGCTACAGAACAGGTTACACGTATCGCCACCCCTTTGTTCAAGAGAAAACCAAACACAAAAGTGA
  GGTGGAGATTCCAGCCACGGTCACAGCCTTCGTGTTTGAGGAGGACGGTGCCCCGGTGCCCGCCCTGCGA
  CAGCATGCCCAGAAGCAGCAGAAGGAGAAGACCCGCTGGCTCAATACGCCCAACGCCTACCTGCGGGTCA
  ATGTGGCCGATGAGGTCCAGAGGAGCATGGGCAGCCCCCGACCCAAGACCACGTGGATGAAGGCTGACGA
  GGTGGAGAAATCCAGCAGTGGCATGCCGATTCGCATCGAAAACCCAAACCAATTTGTGCCTCTCTATACT
  GACCCCCAGGAAGTACTGGAGATGAGGAACAAGATTCGAGAACAAAACCGACAAGATGTGAAGTCAGCGG
  GGCCTCAGTCCCAGCTCCTGGCGAGCGTCATTGCCGAGAAGAGCCGAAGCCCGTCTACAGAGAGCCAGCT
  GATGTCCAAGGGAGACGAGGATACCAAAGACGATTCAGAGGAGACGGTGCCCAACCCCTTCAGCCAACTC
  ACTGACCAGGAGTTGGAGGAGTACAAGAAAGAGGTGGAGAGGAAGAAACTAGAACTTGATGGAGAGAAAG
  AAACTGCCCCAGAAGAGCCTGGCTCACCTGCAAAGTCTGCACCTGCTTCTCCAGTGCAGAGCCCAGCGAA
  GGAGGCAGAGACAAAGAGCCCTTTAGTCTCTCCTTCCAAGTCTTTAGAGGAAGGTACTAAGAAGACAGAA
  ACAAGCAAAGCCGCCACCACAGAGCCCGAAACAACCCAGCCGGAAGGGGTGGTGGTCAACGGGAGGGAGG
  AGGAGCAGACGGCAGAGGAAATCCTCAGCAAAGGCCTGAGCCAGATGACCACCAGTGCTGACACGGATGT
  TGATACCTCTAAGGACAAAACCGAGTCGGTCACCAGCGGCCCCATGTCCCCAGAGGGCTCACCTTCCAAG
  TCTCCCTCAAAGAAGAAAAAGAAATTCCGAACCCCCTCCTTCCTGAAAAAGAGCAAAAAGAAGGAGAAAG
  TGGAGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209517 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGL
  IQEQMKKGNNSSNIWALRQIADFMASTSHAVFPTSSMNVSMMTPINDLHTADSLNLAKGERLMRCKISSV
  YRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFC
  LHSAIYAARPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGP
  TCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSALSSAGGVENLILLEQEKHRPHEVGSVQWAGS
  TFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGAPVPALR
  QHAQKQQKEKTRWLNTPNAYLRVNVADEVQRSMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYT
  DPQEVLEMRNKIREQNRQDVKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDEDTKDDSEETVPNPFSQL
  TDQELEEYKKEVERKKLELDGEKETAPEEPGSPAKSAPASPVQSPAKEAETKSPLVSPSKSLEEGTKKTE
  TSKAATTEPETTQPEGVVVNGREEEQTAEEILSKGLSQMTTSADTDVDTSKDKTESVTSGPMSPEGSPSK
  SPSKKKKKFRTPSFLKKSKKKEKVES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001617
• ORF Size: 2178 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001617.1, NM_001617.2, NM_001617.3, NP_001608.1
• RefSeq Size: 4033 bp
• RefSeq ORF: 2181 bp
• Locus ID: 119
• Cytogenetics: 2p13.3
• Domains: Aldolase_II
• MW: 80.8 kDa
• Gene Summary: 'Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010]'