PNPLA3 (NM_025225) Human Tagged ORF Clone

CAT#: RC209577

PNPLA3 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 3 (PNPLA3)

Product Images

Western blot validation of overexpression lysate (Cat# LY410772) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209577 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PNPLA3 protein (Cat# TP309577). The protein was produced from HEK293T cells transfected with PNPLA3 cDNA clone (Cat# RC209577) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PNPLA3
• Synonyms: ADPN; C22orf20; iPLA(2)epsilon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209577 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACGACGCAGAGCGCGGCTGGAGCTTGTCCTTCGCGGGCTGCGGCTTCCTGGGCTTCTACCACGTCG
  GGGCGACCCGCTGCCTGAGCGAGCACGCCCCGCACCTCCTCCGCGACGCGCGCATGTTGTTCGGCGCTTC
  GGCCGGGGCGTTGCACTGCGTCGGCGTCCTCTCCGGTATCCCGCTGGAGCAGACTCTGCAGGTCCTCTCA
  GATCTTGTGCGGAAGGCCAGGAGTCGGAACATTGGCATCTTCCATCCATCCTTCAACTTAAGCAAGTTCC
  TCCGACAGGGTCTCGGCAAATGCCTCCCGGCCAATGTCCACCAGCTCATCTCCGGCAAAATAGGCATCTC
  TCTTACCAGAGTGTCTGATGGGGAAAACGTTCTGGTGTCTGACTTTCGGTCCAAAGACGAAGTCGTGGAT
  GCCTTGGTATGTTCCTGCTTCATGCCTTTCTACAGTGGCCTTATCCCTCCTTCCTTCAGAGGCGTGCGAT
  ATGTGGATGGAGGAGTGAGTGACAACGTACCCTTCATTGATGCCAAAACAACCATCACCGTGTCCCCCTT
  CTATGGGGAGTACGACATCTGCCCTAAAGTCAAGTCCACGAACTTTCTTCATGTGGACATCACCAAGCTC
  AGTCTACGCCTCTGCACAGGGAACCTCTACCTTCTCTCGAGAGCTTTTGTCCCCCCGGATCTCAAGGTGC
  TGGGAGAGATATGCCTTCGAGGATATTTGGATGCATTCAGGTTCTTGGAAGAGAAGGGCATCTGCAACAG
  GCCCCAGCCAGGCCTGAAGTCATCCTCAGAAGGGATGGATCCTGAGGTCGCCATGCCCAGCTGGGCAAAC
  ATGAGTCTGGATTCTTCCCCGGAGTCGGCTGCCTTGGCTGTGAGGCTGGAGGGAGATGAGCTGCTAGACC
  ACCTGCGTCTCAGCATCCTGCCCTGGGATGAGAGCATCCTGGACACCCTCTCGCCCAGGCTCGCTACAGC
  ACTGAGTGAAGAAATGAAAGACAAAGGTGGATACATGAGCAAGATTTGCAACTTGCTACCCATTAGGATA
  ATGTCTTATGTAATGCTGCCCTGTACCCTGCCTGTGGAATCTGCCATTGCGATTGTCCAGAGACTGGTGA
  CATGGCTTCCAGATATGCCCGACGATGTCCTGTGGTTGCAGTGGGTGACCTCACAGGTGTTCACTCGAGT
  GCTGATGTGTCTGCTCCCCGCCTCCAGGTCCCAAATGCCAGTGAGCAGCCAACAGGCCTCCCCATGCACA
  CCTGAGCAGGACTGGCCCTGCTGGACTCCCTGCTCCCCCGAGGGCTGTCCAGCAGAGACCAAAGCAGAGG
  CCACCCCGCGGTCCATCCTCAGGTCCAGCCTGAACTTCTTCTTGGGCAATAAAGTACCTGCTGGTGCTGA
  GGGGCTCTCCACCTTTCCCAGTTTTTCACTAGAGAAGAGTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209577 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYDAERGWSLSFAGCGFLGFYHVGATRCLSEHAPHLLRDARMLFGASAGALHCVGVLSGIPLEQTLQVLS
  DLVRKARSRNIGIFHPSFNLSKFLRQGLGKCLPANVHQLISGKIGISLTRVSDGENVLVSDFRSKDEVVD
  ALVCSCFMPFYSGLIPPSFRGVRYVDGGVSDNVPFIDAKTTITVSPFYGEYDICPKVKSTNFLHVDITKL
  SLRLCTGNLYLLSRAFVPPDLKVLGEICLRGYLDAFRFLEEKGICNRPQPGLKSSSEGMDPEVAMPSWAN
  MSLDSSPESAALAVRLEGDELLDHLRLSILPWDESILDTLSPRLATALSEEMKDKGGYMSKICNLLPIRI
  MSYVMLPCTLPVESAIAIVQRLVTWLPDMPDDVLWLQWVTSQVFTRVLMCLLPASRSQMPVSSQQASPCT
  PEQDWPCWTPCSPEGCPAETKAEATPRSILRSSLNFFLGNKVPAGAEGLSTFPSFSLEKSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025225
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025225.2, NP_079501.2
• RefSeq Size: 2805 bp
• RefSeq ORF: 1446 bp
• Locus ID: 80339
• Domains: Patatin
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Limonene and pinene degradation, Metabolic pathways, Phenylalanine metabolism, Tyrosine metabolism
• MW: 52.8 kDa
• Gene Summary: The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008]