BARX1 (NM_021570) Human Tagged ORF Clone

CAT#: RC209592

BARX1 (Myc-DDK-tagged)-Human BARX homeobox 1 (BARX1)

Product Images

Western blot validation of overexpression lysate (Cat# LY411984) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209592 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BARX1
• Synonyms: BarH-like homeobox 1; BARX homeobox 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209592 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGGCCGGGGGAGCCGGGCGCCGCGCGCTTCGGCCCGCCCGAGGGCTGCGCGGACCACCGGCCGC
  ACCGCTATCGCAGCTTCATGATTGAGGAGATCCTCACGGAGCCACCCGGGCCCAAGGGCGCCGCGCCCGC
  AGCCGCCGCTGCCGCGGCGGGCGAGCTGCTGAAGTTCGGCGTGCAGGCGCTGCTGGCGGCGCGGCCCTTC
  CACAGCCACCTGGCCGTGCTGAAGGCCGAGCAGGCGGCGGTGTTCAAGTTCCCACTGGCGCCGCTGGGCT
  GTTCAGGGCTGAGCTCTGCGTTGCTGGCGGCAGGGCCCGGGCTGCCCGGCGCCGCGGGTGCGCCACACCT
  GCCGCTCGAGTTGCAGCTCCGCGGGAAGCTGGAGGCGGCAGGCCCTGGGGAGCCAGGCACCAAAGCCAAG
  AAGGGGCGTCGGAGCCGCACTGTGTTCACCGAGCTGCAGCTGATGGGCCTGGAGAAACGCTTCGAGAAGC
  AGAAGTACCTTTCCACGCCGGACAGAATAGATCTTGCTGAGTCCCTGGGCCTGAGCCAGTTGCAGGTGAA
  GACGTGGTACCAGAATCGGAGGATGAAGTGGAAGAAAATAGTGCTGCAGGGCGGCGGCCTGGAGTCTCCC
  ACCAAGCCCAAGGGGCGGCCCAAGAAGAACTCAATTCCAACGAGCGAGCAGCTTACTGAGCAGGAGCGTG
  CCAAGGATGCAGAGAAACCGGCGGAGGTGCCGGGCGAGCCCAGCGACAGGAGCCGCGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209592 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQRPGEPGAARFGPPEGCADHRPHRYRSFMIEEILTEPPGPKGAAPAAAAAAAGELLKFGVQALLAARPF
  HSHLAVLKAEQAAVFKFPLAPLGCSGLSSALLAAGPGLPGAAGAPHLPLELQLRGKLEAAGPGEPGTKAK
  KGRRSRTVFTELQLMGLEKRFEKQKYLSTPDRIDLAESLGLSQLQVKTWYQNRRMKWKKIVLQGGGLESP
  TKPKGRPKKNSIPTSEQLTEQERAKDAEKPAEVPGEPSDRSRED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021570
• ORF Size: 762 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021570.3, NP_067545.3
• RefSeq Size: 1498
• RefSeq ORF: 765
• Locus ID: 56033
• Protein Families: Transcription Factors
• MW: 27.3 kDa
• Gene Summary: This gene encodes a member of the Bar subclass of homeobox transcription factors. Studies of the mouse and chick homolog suggest the encoded protein may play a role in developing teeth and craniofacial mesenchyme of neural crest origin. The protein may also be associated with differentiation of stomach epithelia. [provided by RefSeq, Jul 2008]