SEPTIN6 (NM_145799) Human Tagged ORF Clone

CAT#: RC209634

SEPT6 (Myc-DDK-tagged)-Human septin 6 (SEPT6), transcript variant I

Product Images

Western blot validation of overexpression lysate (Cat# LY407883) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209634 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SEPTIN6 protein (Cat# TP309634). The protein was produced from HEK293T cells transfected with SEPTIN6 cDNA clone (Cat# RC209634) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEPTIN6
• Synonyms: SEP2; SEPT2; SEPT6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209634 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGACCGATATAGCTCGCCAGGTGGGTGAAGGTTGCCGAACTGTCCCCCTGGCTGGACATGTGG
  GGTTTGACAGCTTGCCTGACCAGCTGGTGAATAAGTCCGTCAGCCAGGGCTTCTGCTTCAACATCCTGTG
  CGTGGGAGAGACAGGTTTGGGCAAGTCCACCCTCATGGACACCCTGTTCAACACCAAATTCGAAGGGGAG
  CCAGCCACCCACACACAGCCGGGTGTCCAGCTCCAGTCTAATACCTATGACCTCCAAGAGAGCAACGTGA
  GGCTAAAGCTCACGATCGTTAGCACAGTTGGCTTTGGGGACCAGATCAACAAAGAGGACAGCTACAAGCC
  TATCGTGGAATTCATCGATGCACAATTCGAGGCCTACCTGCAGGAAGAGCTAAAGATCCGAAGAGTGCTA
  CACACCTACCATGACTCCCGAATCCATGTCTGCTTGTATTTCATTGCCCCCACGGGTCATTCCCTGAAGT
  CTCTGGACCTAGTGACTATGAAGAAGCTGGACAGTAAGGTGAACATCATCCCCATCATTGCCAAAGCAGA
  TGCCATTTCGAAGAGTGAGCTAACAAAGTTCAAAATCAAAATCACCAGCGAGCTTGTCAGCAACGGAGTC
  CAGATCTATCAGTTTCCTACAGATGATGAGTCGGTGGCAGAGATCAATGGAACCATGAACGCCCACCTGC
  CGTTTGCTGTCATTGGCAGCACAGAAGAACTGAAGATAGGCAACAAGATGATGAGGGCGCGGCAGTATCC
  TTGGGGCACTGTGCAGGTTGAAAACGAGGCCCACTGCGACTTTGTGAAGCTGCGGGAGATGCTGATTCGG
  GTCAACATGGAGGATCTGCGGGAGCAGACCCACACCCGGCACTATGAGCTGTATCGCCGCTGTAAGCTGG
  AGGAGATGGGCTTCAAGGACACCGACCCTGACAGCAAACCCTTCAGTTTACAGGAGACACATGAGGCCAA
  AAGGAACGAGTTCCTAGGGGAACTCCAGAAAAAAGAAGAGGAGATGAGACAGATGTTCGTCCAGCGAGTC
  AAAGAGAAAGAAGCGGAGCTCAAAGAGGCAGAGAAAGAGCTGCACGAGAAGTTTGACCGTCTGAAGAAAC
  TGCACCAGGACGAGAAGAAGAAACTGGAGGATAAGAAGAAATCCCTGGATGATGAAGTGAATGCTTTCAA
  GCAAAGAAAGACGGCGGCTGAGCTGCTCCAGTCCCAGGGCTCCCAGGCTGGAGGCTCACAGACTCTGAAG
  AGAGACAAAGAGAAGAAAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209634 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAATDIARQVGEGCRTVPLAGHVGFDSLPDQLVNKSVSQGFCFNILCVGETGLGKSTLMDTLFNTKFEGE
  PATHTQPGVQLQSNTYDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVL
  HTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADAISKSELTKFKIKITSELVSNGV
  QIYQFPTDDESVAEINGTMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIR
  VNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETHEAKRNEFLGELQKKEEEMRQMFVQRV
  KEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEDKKKSLDDEVNAFKQRKTAAELLQSQGSQAGGSQTLK
  RDKEKKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145799
• ORF Size: 1281 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145799.1, NM_145799.2, NM_145799.3, NP_665798.1
• RefSeq Size: 4716 bp
• RefSeq ORF: 1284 bp
• Locus ID: 23157
• MW: 48.8 kDa
• Gene Summary: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]