AMFR (NM_001144) Human Tagged ORF Clone
CAT#: RC209639
AMFR (Myc-DDK-tagged)-Human autocrine motility factor receptor (AMFR)
"NM_001144" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | AMFR |
Synonyms | GP78; RNF45 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209639 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCTGCTCTTCCTCGAGCGCTTCCCCTGGCCCAGCCTCCGCACCTACACGGGCCTCAGCGGCCTGG CCCTGCTGGGCACCATCATCAGCGCCTACCGCGCGCTCAGCCAGCCCGAGGCCGGCCCCGGCGAGCCGGA CCAGCTAACGGCCTCGCTGCAGCCTGAGCCGCCGGCGCCCGCCCGGCCGAGCGCCGGGGGACCCCGGGCC CGCGATGTGGCCCAGTACCTGCTCTCAGACAGCCTCTTCGTGTGGGTTCTAGTAAATACCGCTTGCTGTG TTTTGATGTTGGTGGCTAAGCTCATCCAGTGTATTGTGTTTGGCCCTCTTCGAGTGAGTGAGAGACAGCA TCTCAAAGACAAATTTTGGAATTTTATTTTCTACAAGTTCATTTTCATCTTTGGTGTGCTGAATGTCCAG ACAGTGGAAGAGGTGGTCATGTGGTGCCTCTGGTTTGCCGGACTTGTCTTTCTGCACCTGATGGTTCAGC TCTGCAAGGATCGATTTGAATATCTTTCCTTCTCGCCCACCACGCCGATGAGCAGCCACGGTCGAGTCCT GTCCCTGTTGGTTGCCATGCTGCTTTCCTGCTGTGGACTGGCGGCCGTCTGCTCCATCACCGGCTACACC CACGGAATGCACACCTTGGCTTTCATGGCTGCAGAGTCTCTTCTTGTGACAGTGAGGACTGCTCATGTGA TTTTACGATACGTAATTCACCTCTGGGACCTCAACCACGAAGGGACGTGGGAAGGAAAGGGGACGTATGT CTATTACACAGACTTTGTCATGGAGCTCACTCTCCTGTCCCTGGACCTCATGCACCATATTCACATGTTG TTATTTGGCAACATCTGGTTATCCATGGCCAGCCTGGTCATCTTTATGCAGCTGCGTTACCTGTTTCATG AGGTGCAACGTCGAATTCGTCGGCACAAGAACTATCTACGTGTGGTTGGAAACATGGAGGCCAGGTTTGC AGTTGCAACTCCAGAGGAGCTGGCTGTCAACAATGACGACTGTGCCATCTGTTGGGACTCCATGCAGGCT GCGCGGAAACTGCCCTGTGGACATCTTTTCCACAACTCCTGTCTTCGTTCCTGGCTAGAACAAGACACCT CCTGTCCAACATGCAGAATGTCTCTTAATATTGCCGACAATAATCGTGTCAGGGAAGAACATCAAGGAGA GAACTTGGATGAGAATTTGGTTCCTGTAGCAGCAGCCGAAGGGAGACCTCGCTTAAACCAACACAATCAC TTCTTCCATTTCGATGGGTCTCGGATTGCGAGCTGGCTGCCGAGTTTTTCGGTTGAAGTGATGCACACCA CCAACATTCTTGGCATTACGCAGGCCAGCAACTCCCAGCTCAATGCAATGGCTCATCAGATTCAAGAGAT GTTTCCCCAGGTTCCATACCATCTGGTACTGCAGGACCTCCAGCTGACACGCTCAGTTGAAATAACAACA GACAATATTTTAGAAGGACGGATTCAAGTACCTTTTCCTACACAGCGGTCAGATAGCATCAGACCTGCAT TGAACAGTCCTGTGGAAAGGCCAAGCAGTGACCAGGAAGAGGGAGAAACTTCTGCTCAGACCGAGCGTGT GCCACTGGACCTCAGTCCTCGCCTGGAGGAGACGCTGGACTTCGGCGAGGTGGAAGTGGAGCCCAGTGAG GTGGAAGACTTCGAGGCTCGTGGGAGCCGCTTCTCCAAGTCTGCTGATGAGAGACAGCGCATGCTGGTGC AGCGTAAGGACGAACTCCTCCAGCAAGCTCGCAAACGTTTCTTGAACAAAAGTTCTGAAGATGATGCGGC CTCAGAGAGCTTCCTCCCCTCGGAAGGTGCGTCCTCTGACCCCGTGACCCTGCGTCGAAGGATGCTGGCT GCCGCCGCGGAACGGAGGCTTCAGAAGCAGCAGACCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209639 protein sequence
Red=Cloning site Green=Tags(s) MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRA RDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQ TVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYT HGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHML LFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQA ARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNH FFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITT DNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSE VEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLA AAAERRLQKQQTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001144 |
ORF Size | 1929 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001144.2, NM_001144.3, NM_001144.4, NM_001144.5, NP_001135.3 |
RefSeq Size | 3631 bp |
RefSeq ORF | 1932 bp |
Locus ID | 267 |
Cytogenetics | 16q13 |
Domains | RING, CUE |
Protein Families | Druggable Genome, Transmembrane |
MW | 73 kDa |
Gene Summary | 'This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119417 | AMFR (untagged)-Human autocrine motility factor receptor (AMFR) |
USD 310.00 |
|
RG209639 | AMFR (GFP-tagged) - Human autocrine motility factor receptor (AMFR) |
USD 460.00 |
|
RC209639L1 | Lenti ORF clone of Human autocrine motility factor receptor (AMFR), Myc-DDK-tagged |
USD 768.00 |
|
RC209639L2 | Lenti ORF clone of Human autocrine motility factor receptor (AMFR), mGFP tagged |
USD 620.00 |
|
RC209639L3 | Lenti ORF clone of Human autocrine motility factor receptor (AMFR), Myc-DDK-tagged |
USD 620.00 |
|
RC209639L4 | Lenti ORF clone of Human autocrine motility factor receptor (AMFR), mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review