WEE1 (NM_003390) Human Tagged ORF Clone

CAT#: RC209760

WEE1 (Myc-DDK-tagged)-Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418682) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209760 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified WEE1 protein (Cat# TP309760). The protein was produced from HEK293T cells transfected with WEE1 cDNA clone (Cat# RC209760) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WEE1
• Synonyms: WEE1A; WEE1hu
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209760 representing NM_003390
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCACCCCGCCGCGCCGGGGCGGCCTGCACCTTGCGGCAGA
  AGCTGATCTTCTCGCCCTGCAGCGACTGTGAGGAGGAGGAAGAAGAGGAGGAGGAGGAGGGCAGCGGCCA
  CAGCACCGGGGAGGACTCGGCCTTTCAAGAGCCCGACTCGCCGCTGCCGCCCGCGCGGAGCCCCACGGAG
  CCCGGGCCCGAGCGCCGCCGCTCGCCCGGGCCGGCCCCCGGGAGCCCCGGCGAGCTGGAGGAGGACCTGT
  TGCTGCCCGGCGCCTGCCCGGGCGCGGACGAGGCGGGCGGTGGGGCGGAGGGCGACTCGTGGGAGGAGGA
  GGGCTTCGGCTCCTCGTCGCCGGTCAAGTCGCCGGCGGCCCCCTACTTCCTGGGTAGCTCTTTCTCGCCG
  GTGCGCTGCGGCGGCCCAGGAGATGCGTCGCCGCGGGGTTGCGGGGCGCGCCGGGCGGGCGAAGGCCGCC
  GCTCGCCGCGGCCGGACCACCCGGGCACCCCGCCACACAAGACCTTCCGCAAGCTGCGACTCTTCGACAC
  CCCGCACACGCCCAAGAGTTTGCTCTCCAAAGCTCGGGGAATTGATTCCAGCTCTGTTAAACTCCGGGGT
  AGTTCTCTCTTCATGGATACAGAAAAATCAGGAAAAAGGGAATTTGATGTGCGACAGACTCCTCAAGTGA
  ATATTAATCCTTTTACTCCGGATTCTTTGTTGCTTCATTCCTCAGGACAGTGTCGTCGTAGAAAGAGAAC
  GTATTGGAATGATTCCTGTGGTGAAGACATGGAAGCCAGTGATTATGAGCTTGAAGATGAAACAAGACCT
  GCTAAGAGAATTACAATTACTGAAAGCAATATGAAGTCCCGGTATACAACAGAATTTCATGAGCTAGAGA
  AAATCGGCTCTGGAGAATTTGGTTCTGTATTTAAGTGTGTGAAGAGGCTGGATGGATGCATTTATGCCAT
  TAAGCGATCAAAAAAGCCATTGGCGGGCTCTGTTGATGAGCAGAACGCTTTGAGAGAAGTATATGCTCAT
  GCAGTGCTTGGACAGCATTCTCATGTAGTTCGATATTTCTCTGCGTGGGCAGAAGATGATCATATGCTTA
  TACAGAATGAATATCGTAATGGTGGAAGTTTAGCTGATGCTATAAGTGAAAACTACAGAATCATGAGTTA
  CTTTAAAGAAGCAGAGTTGAAGGATCTCCTTTTGCAAGTTGGCCGAGGCTTGAGGTATATTCATTCAATG
  TCTTTGGTTCACATGGATATAAAACCTAGTAATATTTTCATATCTCGAACCTCAATCCCAAATGCTGCCT
  CTGAAGAAGGAGACGAAGATGATTGGGCATCCAACAAAGTTATGTTTAAAATAGGTGATCTTGGGCATGT
  AACAAGGATCTCCAGTCCACAAGTTGAAGAGGGCGATAGTCGTTTTCTTGCAAATGAAGTTTTACAGGAG
  AATTATACCCATCTACCAAAAGCAGATATTTTTGCGCTTGCCCTCACAGTGGTATGTGCTGCTGGTGCTG
  AACCTCTTCCGAGAAATGGAGATCAATGGCATGAAATCAGACAGGGTAGATTACCTCGGATACCACAAGT
  GCTTTCCCAAGAATTTACAGAGTTGCTAAAAGTTATGATTCATCCAGATCCAGAGAGAAGACCTTCAGCA
  ATGGCACTGGTAAAGCATTCAGTATTGCTGTCCGCTTCTAGAAAGAGTGCAGAACAATTACGAATAGAAT
  TGAATGCCGAAAAGTTCAAAAATTCACTTTTACAAAAAGAACTCAAGAAAGCACAGATGGCAAAAGCTGC
  AGCTGAGGAAAGAGCACTCTTCACTGACCGGATGGCCACTAGGTCCACCACCCAGAGTAATAGAACATCT
  CGACTTATTGGAAAGAAAATGAACCGCTCTGTCAGCCTTACTATATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209760 representing NM_003390
  Red=Cloning site Green=Tags(s)
  
  MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTE
  PGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSP
  VRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRG
  SSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRP
  AKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAH
  AVLGQHSHVVRYFSAWAEDDHMLIQNEYRNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSM
  SLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQE
  NYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSA
  MALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTS
  RLIGKKMNRSVSLTIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003390
• ORF Size: 1938 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003390.1, NM_003390.2, NM_003390.3, NP_003381.1
• RefSeq Size: 4232 bp
• RefSeq ORF: 1941 bp
• Locus ID: 7465
• Cytogenetics: 11p15.4
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• MW: 71.4 kDa
• Gene Summary: 'This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]'