WEE1 (NM_003390) Human Untagged Clone

CAT#: SC117999

WEE1 (untagged)-Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WEE1
• Synonyms: WEE1A; WEE1hu
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003390 edited
  ATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCACCCCGCCGCGCCGGGGCGGCCTGCACC
  TTGCGGCAGAAGCTGATCTTCTCGCCCTGCAGCGACTGTGAGGAGGAGGAAGAAGAGGAG
  GAGGAGGAGGGCAGCGGCCACAGCACCGGGGAGGACTCGGCCTTTCAAGAGCCCGACTCG
  CCGCTGCCGCCCGCGCGGAGCCCCACGGAGCCCGGGCCCGAGCGCCGCCGCTCGCCCGGG
  CCGGCCCCCGGGAGCCCCGGCGAGCTGGAGGAGGACCTGTTGCTGCCCGGCGCCTGCCCG
  GGCGCGGACGAGGCGGGCGGTGGGGCGGAGGGCGACTCGTGGGAGGAGGAGGGCTTCGGC
  TCCTCGTCGCCGGTCAAGTCGCCGGCGGCCCCCTACTTCCTGGGTAGCTCTTTCTCGCCG
  GTGCGCTGCGGCGGCCCAGGAGATGCGTCGCCGCGGGGTTGCGGGGCGCGCCGGGCGGGC
  GAAGGCCGCCGCTCGCCGCGGCCGGACCACCCGGGCACCCCGCCACACAAGACCTTCCGC
  AAGCTGCGACTCTTCGACACCCCGCACACGCCCAAGAGTTTGCTCTCCAAAGCTCGGGGA
  ATTGATTCCAGCTCTGTTAAACTCCGGGGTAGTTCTCTCTTCATGGATACAGAAAAATCA
  GGAAAAAGGGAATTTGATGTGCGACAGACTCCTCAAGTGAATATTAATCCTTTTACTCCG
  GATTCTTTGTTGCTTCATTCCTCAGGACAGTGTCGTCGTAGAAAGAGAACGTATTGGAAT
  GATTCCTGTGGTGAAGACATGGAAGCCAGTGATTATGAGCTTGAAGATGAAACAAGACCT
  GCTAAGAGAATTACAATTACTGAAAGCAATATGAAGTCCCGGTATACAACAGAATTTCAT
  GAGCTAGAGAAAATCGGCTCTGGAGAATTTGGTTCTGTATTTAAGTGTGTGAAGAGGCTG
  GATGGATGCATTTATGCCATTAAGCGATCAAAAAAGCCATTGGCGGGCTCTGTTGATGAG
  CAGAACGCTTTGAGAGAAGTATATGCTCATGCAGTGCTTGGACAGCATTCTCATGTAGTT
  CGATATTTCTCTGCGTGGGCAGAAGATGATCATATGCTTATACAGAATGAATATTGTAAT
  GGTGGAAGTTTAGCTGATGCTATAAGTGAAAACTACAGAATCATGAGTTACTTTAAAGAA
  GCAGAGTTGAAGGATCTCCTTTTGCAAGTTGGCCGAGGCTTGAGGTATATTCATTCAATG
  TCTTTGGTTCACATGGATATAAAACCTAGTAATATTTTCATATCTCGAACCTCAATCCCA
  AATGCTGCCTCTGAAGAAGGAGACGAAGATGATTGGGCATCCAACAAAGTTATGTTTAAA
  ATAGGTGATCTTGGGCATGTAACAAGGATCTCCAGTCCACAAGTTGAAGAGGGCGATAGT
  CGTTTTCTTGCAAATGAAGTTTTACAGGAGAATTATACCCATCTACCAAAAGCAGATATT
  TTTGCGCTTGCCCTCACAGTGGTATGTGCTGCTGGTGCTGAACCTCTTCCGAGAAATGGA
  GATCAATGGCATGAAATCAGACAGGGTAGATTACCTCGGATACCACAAGTGCTTTCCCAA
  GAATTTACAGAGTTGCTAAAAGTTATGATTCATCCAGATCCAGAGAGAAGACCTTCAGCA
  ATGGCACTGGTAAAGCATTCAGTATTGCTGTCCGCTTCTAGAAAGAGTGCAGAACAATTA
  CGAATAGAATTGAATGCCGAAAAGTTCAAAAATTCACTTTTACAAAAAGAACTCAAGAAA
  GCACAGATGGCAAAAGCTGCAGCTGAGGAAAGAGCACTCTTCACTGACCGGATGGCCACT
  AGGTCCACCACCCAGAGTAATAGAACATCTCGACTTATTGGAAAGAAAATGAACCGCTCT
  GTCAGCCTTACTATATACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003390 unedited
  GATAGCGCTGCGGTNGAAAACGAATCCGGGGGGCGGAAAAACCTTCGCACGGGGCCGCCC
  CGTTTCTGCCGGAAGTCCGCGCCGCCGCTGCCGCCACCGTCCGCAGCCCGAGCGCCCCGG
  AGCCGCAGGCCGCCGCCGCGCAGAGACGCCGCGGCTGCGACTAGGCGCGCCCAGCCGCAC
  GTGGCGGACCCGCCCCCAGGCCCGCAGTGTTTTTTTATTCCGCAGGCCTCCGCTCTCCTG
  TCCTCGGCCCCGTCCCCAGGGCCGCGATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCAC
  CCCGCCGCGCCGGGGCGGCCTGCACCTTGCGGCAGAAGCTGATCTTCTCGCCCTGCAGCG
  ACTGTGAGGAGGAGGAAGAAGAGGAGGAGGAGGAGGGCAGCGGCCACAGCACCGGGGAGG
  ACTCGGCCTTTCAAGAGCCCGACTCGCCGCTGCCGCCCGCGCGGAGCCCCACGGAGCCCG
  GGCCCGAGCGCCGCCGCTCGCCCGGGCCGGCCCCCGGGAGCCCCGGCGAGCTGGAGGAGG
  ACCTGTTGCTGCCCGGCGCCTGCCCGGGCGCGGACGAGGCGGGCGGTGGGGCGGAGGGCG
  ACTCGTGGGAGGAGGAGGGCTTCGGCTCCTCGTCGCCGGTCAAGTCGCCGGCGGCCCCCT
  ACTTCCTGGGGAGCTCTTTCTCGCCGGTGCGCTGCGGCGGCCCAGGAGATGCCTCCCCGC
  GGGGTTGCGGGAGCGCACGGAGGAGCATGATCCTCTCGCGACGCTGGGAGTGATACGGCG
  GGGGGAGGGAGGGGGAGGAGCAGGCATGAGGGAGAGGTCGGGCGGGCGAGGCCCGGGGGC
  AGGAGGGAGTGGAGGGGGGGGGGGAGGGAGGGAGGGTGGGGTGGTGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003390 unedited
  TGGACCGCGGCACGCAATCTAGTAGTCAGNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTAAAACCCAAAGAGACTTTTAATATGCCAATCAATGTTAATAAAACACAAG
  TCAAAAACAAGGGCAAACATGTTTTAAACCAAATTTAATGGAAAAAACAAACAGACATTT
  TTTTTCAACATCTGTTAGCCAGTATTATTAGTCAAATGGCTAATCACAAATAAAATATAT
  TTTGGGAAAAACTTGGAATGGCAAAAGTCATTCTGGCATTTCAAACAGCTATGTACAGTA
  TCACAAAAATCGGTTTATATACACAAATATTGAAAAAAAAAACCGGGCAAAACATTTAAA
  AACAGACTAATAATACAATCAAGTTTAAAACTTAGGGGGAAACATTTAAAAAGAGTCCGG
  GAAGGACATTACCAAAACAAGTTACAAAACCAGGAATTATTATACTACATTAATACAGCA
  TTCCAAAGGAGGGAAAGGGGGCATTTTTCCAATGCACTAGCTTCAAAGTTCAAAGGAAAA
  AAAAAAAAAGGCAAATAGCTGCTTTTCATCAAAAGCAGCTATACATTTCACATAATTGTT
  TCAAAAAGAAGACCTATTAACAACAATGATTGTTTAATGCTACAAGTTTACAGCAAAGAC
  AAAACTAAAATAGCAGCAAATTCACAAGGCATTACCTCCACAGAATCATCATCCCATGGT
  TCATGCAATGCCTACAAAGGGCTCCCAGTGGGATATACAAGTCTAATTCCCATTTAAAAA
  AAAAGGATATATAGATGCACACCCAGGTCCATTGAGGCTGAGTAGTAATGAGTCTTTAGC
  ATGTCCCTTTTAAAGAAGGGTATGTCTGAATAAAGCCCTGATACTTTTTCAGGAACTACA
  GGAAAGAACCTTTTTTACATGCACAGCAACTAAGCGCCCCCCCCCGGGGTAACCTGAAAG
  CTGGGTACTCCACAGGGCACTCTAAAAGAAATGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003390
• Insert Size: 3670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003390.2, NP_003381.1
• RefSeq Size: 4232 bp
• RefSeq ORF: 1941 bp
• Locus ID: 7465
• Cytogenetics: 11p15.4
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• Gene Summary: 'This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).