LSG1 (NM_018385) Human Tagged ORF Clone

CAT#: RC209779

LSG1 (Myc-DDK-tagged)-Human large subunit GTPase 1 homolog (S. cerevisiae) (LSG1)

Product Images

Western blot validation of overexpression lysate (Cat# LY413084) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209779 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LSG1 protein (Cat# TP309779). The protein was produced from HEK293T cells transfected with LSG1 cDNA clone (Cat# RC209779) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LSG1
• Synonyms: FLJ11301; FLJ27294
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209779 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGGAGGAGAGCCCCGGCCGGTGGGTCGCTGGGACGGGCCCTTATGCGCCATCAGACTCAGCGGA
  GCCGAAGCCATCGTCACACTGACTCCTGGTTGCACACAAGTGAACTCAATGATGGCTATGATTGGGGTCG
  TCTTAATCTTCAGTCAGTGACTGAACAGAGCTCCCTTGATGACTTCCTTGCTACTGCAGAACTTGCAGGA
  ACAGAGTTTGTAGCTGAAAAACTTAATATTAAGTTTGTGCCTGCTGAGGCTAGAACTGGACTACTGTCTT
  TCGAGGAGAGCCAGAGAATTAAGAAGCTCCATGAAGAAAACAAACAGTTCTTGTGTATACCGAGGAGACC
  AAACTGGAACCAAAATACTACCCCAGAAGAACTCAAACAAGCAGAGAAAGATAACTTTCTAGAATGGAGA
  CGTCAGCTTGTCCGGCTAGAAGAGGAACAGAAGCTGATATTGACTCCATTTGAACGAAATTTGGACTTTT
  GGCGCCAGCTCTGGAGAGTCATTGAGAGAAGTGATATTGTGGTCCAGATAGTAGATGCTCGAAACCCACT
  CCTGTTTAGATGTGAGGATTTGGAATGTTATGTGAAAGAAATGGATGCCAATAAGGAGAACGTCATTCTG
  ATCAACAAGGCAGACTTGCTGACTGCTGAGCAGCGGAGTGCCTGGGCCATGTACTTCGAAAAAGAAGATG
  TGAAGGTTATTTTCTGGTCAGCTTTGGCCGGAGCCATTCCCCTGAATGGTGACTCTGAGGAAGAGGCAAA
  CAGAGATGATAGACAAAGCAACACAACTGAGTTTGGACATTCCAGTTTCGACCAGGCTGAAATTTCCCAC
  AGTGAATCCGAACATCTCCCAGCTAGGGATTCTCCTTCACTTAGTGAAAATCCCACAACGGATGAAGATG
  ACAGTGAGTATGAGGACTGTCCAGAGGAGGAGGAAGACGACTGGCAGACGTGCTCAGAAGAAGACGGTCC
  CAAGGAAGAGGACTGCAGCCAGGACTGGAAGGAAAGCTCTACTGCAGATTCTGAGGCTCGGAGCAGGAAA
  ACCCCACAGAAGAGGCAGATACACAATTTTAGCCATCTGGTATCCAAGCAGGAGTTACTGGAGCTCTTTA
  AGGAGCTACACACTGGGAGAAAGGTGAAAGATGGGCAACTTACGGTCGGACTGGTGGGCTACCCTAATGT
  TGGTAAGAGTTCAACAATCAACACCATCATGGGCAACAAGAAAGTATCTGTGTCTGCCACACCTGGTCAC
  ACAAAGCACTTTCAGACTCTCTATGTGGAGCCTGGCCTCTGCCTGTGTGACTGTCCTGGCTTGGTGATGC
  CATCTTTTGTGTCTACCAAGGCAGAAATGACTTGCAGCGGAATCCTCCCAATTGATCAGATGAGAGATCA
  TGTTCCTCCTGTATCACTAGTTTGCCAGAATATTCCAAGACATGTTTTAGAAGCTACCTATGGCATTAAC
  ATCATAACGCCTAGAGAGGATGAAGATCCCCACCGACCTCCAACATCGGAAGAACTGTTGACAGCTTATG
  GATACATGCGAGGATTCATGACAGCGCATGGACAGCCAGACCAGCCTCGATCTGCGCGCTACATCCTGAA
  GGACTATGTCAGTGGTAAGCTGCTGTACTGCCATCCTCCTCCTGGAAGAGATCCTGTAACTTTTCAGCAT
  CAACACCAGCGACTCCTAGAGAACAAAATGAACAGTGATGAAATAAAAATGCAGCTAGGCAGAAATAAAA
  AAGCAAAGCAGATTGAAAATATCGTTGACAAAACTTTTTTCCATCAAGAGAATGTGAGGGCTTTGACCAA
  AGGAGTCCAGGCTGTGATGGGTTACAAGCCCGGGAGTGGTGTAGTGACTGCATCCACTGCGAGCTCTGAG
  AACGGGGCGGGGAAGCCCTGGAAAAAACATGGCAACAGAAATAAAAAAGAAAAAAGTCGTAGACTCTACA
  AGCACCTGGATATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209779 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGRRRAPAGGSLGRALMRHQTQRSRSHRHTDSWLHTSELNDGYDWGRLNLQSVTEQSSLDDFLATAELAG
  TEFVAEKLNIKFVPAEARTGLLSFEESQRIKKLHEENKQFLCIPRRPNWNQNTTPEELKQAEKDNFLEWR
  RQLVRLEEEQKLILTPFERNLDFWRQLWRVIERSDIVVQIVDARNPLLFRCEDLECYVKEMDANKENVIL
  INKADLLTAEQRSAWAMYFEKEDVKVIFWSALAGAIPLNGDSEEEANRDDRQSNTTEFGHSSFDQAEISH
  SESEHLPARDSPSLSENPTTDEDDSEYEDCPEEEEDDWQTCSEEDGPKEEDCSQDWKESSTADSEARSRK
  TPQKRQIHNFSHLVSKQELLELFKELHTGRKVKDGQLTVGLVGYPNVGKSSTINTIMGNKKVSVSATPGH
  TKHFQTLYVEPGLCLCDCPGLVMPSFVSTKAEMTCSGILPIDQMRDHVPPVSLVCQNIPRHVLEATYGIN
  IITPREDEDPHRPPTSEELLTAYGYMRGFMTAHGQPDQPRSARYILKDYVSGKLLYCHPPPGRDPVTFQH
  QHQRLLENKMNSDEIKMQLGRNKKAKQIENIVDKTFFHQENVRALTKGVQAVMGYKPGSGVVTASTASSE
  NGAGKPWKKHGNRNKKEKSRRLYKHLDM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018385
• ORF Size: 1974 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018385.1, NM_018385.2, NP_060855.1
• RefSeq Size: 3589 bp
• RefSeq ORF: 1977 bp
• Locus ID: 55341
• Domains: MMR_HSR1
• MW: 75.2 kDa
• Gene Summary: This gene encodes a protein related to the yeast large subunit GTPase 1. The encoded protein is necessary for cell viability and may localize in the endoplasmic reticulum, nucleus and cytoplasm. [provided by RefSeq, Feb 2009]