LSG1 (NM_018385) Human Tagged ORF Clone
CAT#: RC209779
LSG1 (Myc-DDK-tagged)-Human large subunit GTPase 1 homolog (S. cerevisiae) (LSG1)
"NM_018385" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | LSG1 |
Synonyms | FLJ11301; FLJ27294 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209779 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCGGAGGAGAGCCCCGGCCGGTGGGTCGCTGGGACGGGCCCTTATGCGCCATCAGACTCAGCGGA GCCGAAGCCATCGTCACACTGACTCCTGGTTGCACACAAGTGAACTCAATGATGGCTATGATTGGGGTCG TCTTAATCTTCAGTCAGTGACTGAACAGAGCTCCCTTGATGACTTCCTTGCTACTGCAGAACTTGCAGGA ACAGAGTTTGTAGCTGAAAAACTTAATATTAAGTTTGTGCCTGCTGAGGCTAGAACTGGACTACTGTCTT TCGAGGAGAGCCAGAGAATTAAGAAGCTCCATGAAGAAAACAAACAGTTCTTGTGTATACCGAGGAGACC AAACTGGAACCAAAATACTACCCCAGAAGAACTCAAACAAGCAGAGAAAGATAACTTTCTAGAATGGAGA CGTCAGCTTGTCCGGCTAGAAGAGGAACAGAAGCTGATATTGACTCCATTTGAACGAAATTTGGACTTTT GGCGCCAGCTCTGGAGAGTCATTGAGAGAAGTGATATTGTGGTCCAGATAGTAGATGCTCGAAACCCACT CCTGTTTAGATGTGAGGATTTGGAATGTTATGTGAAAGAAATGGATGCCAATAAGGAGAACGTCATTCTG ATCAACAAGGCAGACTTGCTGACTGCTGAGCAGCGGAGTGCCTGGGCCATGTACTTCGAAAAAGAAGATG TGAAGGTTATTTTCTGGTCAGCTTTGGCCGGAGCCATTCCCCTGAATGGTGACTCTGAGGAAGAGGCAAA CAGAGATGATAGACAAAGCAACACAACTGAGTTTGGACATTCCAGTTTCGACCAGGCTGAAATTTCCCAC AGTGAATCCGAACATCTCCCAGCTAGGGATTCTCCTTCACTTAGTGAAAATCCCACAACGGATGAAGATG ACAGTGAGTATGAGGACTGTCCAGAGGAGGAGGAAGACGACTGGCAGACGTGCTCAGAAGAAGACGGTCC CAAGGAAGAGGACTGCAGCCAGGACTGGAAGGAAAGCTCTACTGCAGATTCTGAGGCTCGGAGCAGGAAA ACCCCACAGAAGAGGCAGATACACAATTTTAGCCATCTGGTATCCAAGCAGGAGTTACTGGAGCTCTTTA AGGAGCTACACACTGGGAGAAAGGTGAAAGATGGGCAACTTACGGTCGGACTGGTGGGCTACCCTAATGT TGGTAAGAGTTCAACAATCAACACCATCATGGGCAACAAGAAAGTATCTGTGTCTGCCACACCTGGTCAC ACAAAGCACTTTCAGACTCTCTATGTGGAGCCTGGCCTCTGCCTGTGTGACTGTCCTGGCTTGGTGATGC CATCTTTTGTGTCTACCAAGGCAGAAATGACTTGCAGCGGAATCCTCCCAATTGATCAGATGAGAGATCA TGTTCCTCCTGTATCACTAGTTTGCCAGAATATTCCAAGACATGTTTTAGAAGCTACCTATGGCATTAAC ATCATAACGCCTAGAGAGGATGAAGATCCCCACCGACCTCCAACATCGGAAGAACTGTTGACAGCTTATG GATACATGCGAGGATTCATGACAGCGCATGGACAGCCAGACCAGCCTCGATCTGCGCGCTACATCCTGAA GGACTATGTCAGTGGTAAGCTGCTGTACTGCCATCCTCCTCCTGGAAGAGATCCTGTAACTTTTCAGCAT CAACACCAGCGACTCCTAGAGAACAAAATGAACAGTGATGAAATAAAAATGCAGCTAGGCAGAAATAAAA AAGCAAAGCAGATTGAAAATATCGTTGACAAAACTTTTTTCCATCAAGAGAATGTGAGGGCTTTGACCAA AGGAGTCCAGGCTGTGATGGGTTACAAGCCCGGGAGTGGTGTAGTGACTGCATCCACTGCGAGCTCTGAG AACGGGGCGGGGAAGCCCTGGAAAAAACATGGCAACAGAAATAAAAAAGAAAAAAGTCGTAGACTCTACA AGCACCTGGATATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209779 protein sequence
Red=Cloning site Green=Tags(s) MGRRRAPAGGSLGRALMRHQTQRSRSHRHTDSWLHTSELNDGYDWGRLNLQSVTEQSSLDDFLATAELAG TEFVAEKLNIKFVPAEARTGLLSFEESQRIKKLHEENKQFLCIPRRPNWNQNTTPEELKQAEKDNFLEWR RQLVRLEEEQKLILTPFERNLDFWRQLWRVIERSDIVVQIVDARNPLLFRCEDLECYVKEMDANKENVIL INKADLLTAEQRSAWAMYFEKEDVKVIFWSALAGAIPLNGDSEEEANRDDRQSNTTEFGHSSFDQAEISH SESEHLPARDSPSLSENPTTDEDDSEYEDCPEEEEDDWQTCSEEDGPKEEDCSQDWKESSTADSEARSRK TPQKRQIHNFSHLVSKQELLELFKELHTGRKVKDGQLTVGLVGYPNVGKSSTINTIMGNKKVSVSATPGH TKHFQTLYVEPGLCLCDCPGLVMPSFVSTKAEMTCSGILPIDQMRDHVPPVSLVCQNIPRHVLEATYGIN IITPREDEDPHRPPTSEELLTAYGYMRGFMTAHGQPDQPRSARYILKDYVSGKLLYCHPPPGRDPVTFQH QHQRLLENKMNSDEIKMQLGRNKKAKQIENIVDKTFFHQENVRALTKGVQAVMGYKPGSGVVTASTASSE NGAGKPWKKHGNRNKKEKSRRLYKHLDM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018385 |
ORF Size | 1974 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018385.1, NM_018385.2, NP_060855.1 |
RefSeq Size | 3589 bp |
RefSeq ORF | 1977 bp |
Locus ID | 55341 |
Domains | MMR_HSR1 |
MW | 75.2 kDa |
Gene Summary | This gene encodes a protein related to the yeast large subunit GTPase 1. The encoded protein is necessary for cell viability and may localize in the endoplasmic reticulum, nucleus and cytoplasm. [provided by RefSeq, Feb 2009] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108402 | LSG1 (untagged)-Human large subunit GTPase 1 homolog (S. cerevisiae) (LSG1) |
USD 310.00 |
|
RG209779 | LSG1 (GFP-tagged) - Human large subunit GTPase 1 homolog (S. cerevisiae) (LSG1) |
USD 460.00 |
|
RC209779L3 | Lenti ORF clone of Human large subunit GTPase 1 homolog (S. cerevisiae) (LSG1), Myc-DDK-tagged |
USD 620.00 |
|
RC209779L4 | Lenti ORF clone of Human large subunit GTPase 1 homolog (S. cerevisiae) (LSG1), mGFP tagged |
USD 620.00 |
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