LSG1 (NM_018385) Human Tagged ORF Clone

CAT#: RG209779

LSG1 (GFP-tagged) - Human large subunit GTPase 1 homolog (S. cerevisiae) (LSG1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LSG1
• Synonyms: FLJ11301; FLJ27294
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209779 representing NM_018385
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGGAGGAGAGCCCCGGCCGGTGGGTCGCTGGGACGGGCCCTTATGCGCCATCAGACTCAGCGGA
  GCCGAAGCCATCGTCACACTGACTCCTGGTTGCACACAAGTGAACTCAATGATGGCTATGATTGGGGTCG
  TCTTAATCTTCAGTCAGTGACTGAACAGAGCTCCCTTGATGACTTCCTTGCTACTGCAGAACTTGCAGGA
  ACAGAGTTTGTAGCTGAAAAACTTAATATTAAGTTTGTGCCTGCTGAGGCTAGAACTGGACTACTGTCTT
  TCGAGGAGAGCCAGAGAATTAAGAAGCTCCATGAAGAAAACAAACAGTTCTTGTGTATACCGAGGAGACC
  AAACTGGAACCAAAATACTACCCCAGAAGAACTCAAACAAGCAGAGAAAGATAACTTTCTAGAATGGAGA
  CGTCAGCTTGTCCGGCTAGAAGAGGAACAGAAGCTGATATTGACTCCATTTGAACGAAATTTGGACTTTT
  GGCGCCAGCTCTGGAGAGTCATTGAGAGAAGTGATATTGTGGTCCAGATAGTAGATGCTCGAAACCCACT
  CCTGTTTAGATGTGAGGATTTGGAATGTTATGTGAAAGAAATGGATGCCAATAAGGAGAACGTCATTCTG
  ATCAACAAGGCAGACTTGCTGACTGCTGAGCAGCGGAGTGCCTGGGCCATGTACTTCGAAAAAGAAGATG
  TGAAGGTTATTTTCTGGTCAGCTTTGGCCGGAGCCATTCCCCTGAATGGTGACTCTGAGGAAGAGGCAAA
  CAGAGATGATAGACAAAGCAACACAACTGAGTTTGGACATTCCAGTTTCGACCAGGCTGAAATTTCCCAC
  AGTGAATCCGAACATCTCCCAGCTAGGGATTCTCCTTCACTTAGTGAAAATCCCACAACGGATGAAGATG
  ACAGTGAGTATGAGGACTGTCCAGAGGAGGAGGAAGACGACTGGCAGACGTGCTCAGAAGAAGACGGTCC
  CAAGGAAGAGGACTGCAGCCAGGACTGGAAGGAAAGCTCTACTGCAGATTCTGAGGCTCGGAGCAGGAAA
  ACCCCACAGAAGAGGCAGATACACAATTTTAGCCATCTGGTATCCAAGCAGGAGTTACTGGAGCTCTTTA
  AGGAGCTACACACTGGGAGAAAGGTGAAAGATGGGCAACTTACGGTCGGACTGGTGGGCTACCCTAATGT
  TGGTAAGAGTTCAACAATCAACACCATCATGGGCAACAAGAAAGTATCTGTGTCTGCCACACCTGGTCAC
  ACAAAGCACTTTCAGACTCTCTATGTGGAGCCTGGCCTCTGCCTGTGTGACTGTCCTGGCTTGGTGATGC
  CATCTTTTGTGTCTACCAAGGCAGAAATGACTTGCAGCGGAATCCTCCCAATTGATCAGATGAGAGATCA
  TGTTCCTCCTGTATCACTAGTTTGCCAGAATATTCCAAGACATGTTTTAGAAGCTACCTATGGCATTAAC
  ATCATAACGCCTAGAGAGGATGAAGATCCCCACCGACCTCCAACATCGGAAGAACTGTTGACAGCTTATG
  GATACATGCGAGGATTCATGACAGCGCATGGACAGCCAGACCAGCCTCGATCTGCGCGCTACATCCTGAA
  GGACTATGTCAGTGGTAAGCTGCTGTACTGCCATCCTCCTCCTGGAAGAGATCCTGTAACTTTTCAGCAT
  CAACACCAGCGACTCCTAGAGAACAAAATGAACAGTGATGAAATAAAAATGCAGCTAGGCAGAAATAAAA
  AAGCAAAGCAGATTGAAAATATCGTTGACAAAACTTTTTTCCATCAAGAGAATGTGAGGGCTTTGACCAA
  AGGAGTCCAGGCTGTGATGGGTTACAAGCCCGGGAGTGGTGTAGTGACTGCATCCACTGCGAGCTCTGAG
  AACGGGGCGGGGAAGCCCTGGAAAAAACATGGCAACAGAAATAAAAAAGAAAAAAGTCGTAGACTCTACA
  AGCACCTGGATATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209779 representing NM_018385
  Red=Cloning site Green=Tags(s)
  
  MGRRRAPAGGSLGRALMRHQTQRSRSHRHTDSWLHTSELNDGYDWGRLNLQSVTEQSSLDDFLATAELAG
  TEFVAEKLNIKFVPAEARTGLLSFEESQRIKKLHEENKQFLCIPRRPNWNQNTTPEELKQAEKDNFLEWR
  RQLVRLEEEQKLILTPFERNLDFWRQLWRVIERSDIVVQIVDARNPLLFRCEDLECYVKEMDANKENVIL
  INKADLLTAEQRSAWAMYFEKEDVKVIFWSALAGAIPLNGDSEEEANRDDRQSNTTEFGHSSFDQAEISH
  SESEHLPARDSPSLSENPTTDEDDSEYEDCPEEEEDDWQTCSEEDGPKEEDCSQDWKESSTADSEARSRK
  TPQKRQIHNFSHLVSKQELLELFKELHTGRKVKDGQLTVGLVGYPNVGKSSTINTIMGNKKVSVSATPGH
  TKHFQTLYVEPGLCLCDCPGLVMPSFVSTKAEMTCSGILPIDQMRDHVPPVSLVCQNIPRHVLEATYGIN
  IITPREDEDPHRPPTSEELLTAYGYMRGFMTAHGQPDQPRSARYILKDYVSGKLLYCHPPPGRDPVTFQH
  QHQRLLENKMNSDEIKMQLGRNKKAKQIENIVDKTFFHQENVRALTKGVQAVMGYKPGSGVVTASTASSE
  NGAGKPWKKHGNRNKKEKSRRLYKHLDM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018385
• ORF Size: 1974 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018385.1, NP_060855.1
• RefSeq Size: 3290
• RefSeq ORF: 1977
• Locus ID: 55341
• Domains: MMR_HSR1
• Gene Summary: This gene encodes a protein related to the yeast large subunit GTPase 1. The encoded protein is necessary for cell viability and may localize in the endoplasmic reticulum, nucleus and cytoplasm. [provided by RefSeq, Feb 2009]