ATF6 beta (ATF6B) (NM_004381) Human Tagged ORF Clone

CAT#: RC209917

ATF6B (Myc-DDK-tagged)-Human activating transcription factor 6 beta (ATF6B), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATF6B
• Synonyms: CREB-RP; CREBL1; G13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209917 representing NM_004381.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGAGCTGATGCTGCTCAGCGAGATTGCTGACCCGACGCGTTTCTTCACCGACAACCTGCTTAGC
  CCGGAGGACTGGGGTCTGCAGAACAGCACCTTGTATTCTGGCCTAGATGAAGTGGCCGAGGAGCAGACG
  CAGCTCTTCCGTTGCCCGGAGCAGGATGTCCCGTTTGACGGCAGCTCCCTGGACGTGGGGATGGATGTC
  AGCCCCTCTGAGCCCCCATGGGAACTCCTGCCGATCTTCCCAGATCTTCAGGTGAAGTCTGAGCCATCT
  TCCCCCTGCTCTTCCTCCTCCCTCAGCTCCGAGTCATCGCGTCTCTCCACAGAGCCATCCAGCGAGGCT
  CTTGGGGTAGGGGAGGTGCTCCATGTGAAGACAGAGTCCTTGGCACCCCCACTGTGTCTCCTGGGAGAT
  GACCCAACATCCTCATTTGAAACCGTCCAGATCAACGTTATCCCCACCTCTGATGATTCCTCAGATGTC
  CAGACCAAGATAGAACCTGTCTCTCCATGTTCTTCCGTCAACTCTGAGGCCTCCCTGCTCTCAGCCGAC
  TCCTCCAGCCAGGCTTTTATAGGAGAGGAGGTCCTGGAAGTGAAGACAGAGTCCCTGTCCCCTTCAGGA
  TGCCTCCTGTGGGATGTCCCAGCCCCCTCACTTGGAGCTGTCCAGATCAGCATGGGCCCATCCCTTGAT
  GGCTCCTCAGGCAAAGCCCTGCCCACCCGGAAGCCGCCACTGCAGCCCAAACCTGTAGTGCTAACCACT
  GTCCCAATGCCATCCAGAGCTGTGCCTCCCAGCACCACAGTCCTTCTGCAGTCCCTCGTCCAGCCACCC
  CCAGTGTCCCCAGTTGTCCTCATCCAGGGTGCTATTCGAGTCCAGCCTGAAGGGCCGGCTCCCTCTCTA
  CCACGGCCTGAGAGGAAGAGCATCGTTCCCGCTCCTATGCCTGGAAACTCCTGCCCGCCTGAAGTGGAT
  GCAAAGCTGCTGAAGCGGCAGCAGCGAATGATCAAGAACCGGGAGTCAGCCTGCCAGTCCCGGAGAAAG
  AAGAAAGAGTATCTGCAGGGACTGGAGGCTCGGCTGCAAGCAGTACTGGCTGACAACCAGCAGCTCCGC
  CGAGAGAATGCTGCCCTCCGGCGGCGGCTGGAGGCCCTGCTGGCTGAAAACAGCGAGCTCAAGTTAGGG
  TCTGGAAACAGGAAGGTGGTCTGCATCATGGTCTTCCTTCTCTTCATTGCCTTCAACTTTGGACCTGTC
  AGCATCAGTGAGCCTCCTTCAGCTCCCATCTCTCCTCGGATGAACAAGGGGGAGCCTCAACCCCGGAGA
  CACTTGCTGGGGTTCTCAGAGCAAGAGCCAGTTCAGGGAGTTGAACCTCTCCAGGGGTCCTCCCAGGGC
  CCTAAGGAGCCCCAGCCCAGCCCCACAGACCAGCCCAGTTTCAGCAACCTGACAGCCTTCCCTGGGGGC
  GCCAAGGAGCTACTACTAAGAGACCTAGACCAGCTCTTCCTCTCCTCTGATTGCCGGCACTTCAACCGC
  ACTGAGTCCCTGAGGCTTGCTGACGAGTTGAGTGGCTGGGTCCAGCGCCACCAGAGAGGCCGGAGGAAG
  ATCCCTCAGAGGGCCCAGGAGAGACAGAAGTCTCAGCCACGGAAGAAGTCACCTCCAGTTAAGGCAGTC
  CCCATCCAACCCCCTGGACCCCCAGAAAGGGATTCTGTGGGCCAGCTGCAACTATATCGCCACCCAGAC
  CGTTCGCAGCCAGCATTCTTGGATGCAATTGACCGACGGGAAGACACATTTTATGTTGTCTCTTTCCGA
  AGGGACCACCTGCTGCTCCCAGCCATCAGCCACAACAAGACCTCCCGGCCCAAGATGTCCCTGGTGATG
  CCTGCCATGGCCCCCAATGAGACCCTGTCAGGCCGTGGGGCCCCGGGGGACTATGAGGAGATGATGCAG
  ATCGAGTGTGAGGTCATGGACACCAGGGTGATTCACATCAAGACCTCCACAGTGCCCCCCTCGCTCCGA
  AAACAGCCATCCCCAACCCCAGGCAATGCCACAGGTGGCCCCTTGCCAGTCTCTGCAGCCAGCCAGGCC
  CACCAGGCCTCCCACCAGCCCCTCTACCTCAATCATCCC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC209917
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MAELMLLSEIADPTRFFTDNLLSPEDWGLQNSTLYSGLDEVAEEQTQLFRCPEQDVPFDGSSLDVGMDV
  SPSEPPWELLPIFPDLQVKSEPSSPCSSSSLSSESSRLSTEPSSEALGVGEVLHVKTESLAPPLCLLGD
  DPTSSFETVQINVIPTSDDSSDVQTKIEPVSPCSSVNSEASLLSADSSSQAFIGEEVLEVKTESLSPSG
  CLLWDVPAPSLGAVQISMGPSLDGSSGKALPTRKPPLQPKPVVLTTVPMPSRAVPPSTTVLLQSLVQPP
  PVSPVVLIQGAIRVQPEGPAPSLPRPERKSIVPAPMPGNSCPPEVDAKLLKRQQRMIKNRESACQSRRK
  KKEYLQGLEARLQAVLADNQQLRRENAALRRRLEALLAENSELKLGSGNRKVVCIMVFLLFIAFNFGPV
  SISEPPSAPISPRMNKGEPQPRRHLLGFSEQEPVQGVEPLQGSSQGPKEPQPSPTDQPSFSNLTAFPGG
  AKELLLRDLDQLFLSSDCRHFNRTESLRLADELSGWVQRHQRGRRKIPQRAQERQKSQPRKKSPPVKAV
  PIQPPGPPERDSVGQLQLYRHPDRSQPAFLDAIDRREDTFYVVSFRRDHLLLPAISHNKTSRPKMSLVM
  PAMAPNETLSGRGAPGDYEEMMQIECEVMDTRVIHIKTSTVPPSLRKQPSPTPGNATGGPLPVSAASQA
  HQASHQPLYLNHP
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004381
• ORF Size: 2109 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004381.5
• RefSeq Size: 2622 bp
• RefSeq ORF: 2112 bp
• Locus ID: 1388
• UniProt ID: Q99941
• Cytogenetics: 6p21.32
• Protein Families: Transcription Factors
• MW: 76.7 kDa
• Gene Summary: The protein encoded by this gene is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however, under ER stress, the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]